##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004770
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.72347303362959	32.0	32.0	32.0	32.0	32.0
2	31.393011335927625	32.0	32.0	32.0	32.0	32.0
3	31.532359644495756	32.0	32.0	32.0	32.0	32.0
4	31.63214666042975	32.0	32.0	32.0	32.0	32.0
5	31.588012181892374	32.0	32.0	32.0	32.0	32.0
6	35.23374005991421	36.0	36.0	36.0	36.0	36.0
7	35.24879226091544	36.0	36.0	36.0	36.0	36.0
8	35.20541118863024	36.0	36.0	36.0	36.0	36.0
9	35.281839624988805	36.0	36.0	36.0	36.0	36.0
10	35.1792917782179	36.0	36.0	36.0	36.0	36.0
11	35.30830737382684	36.0	36.0	36.0	36.0	36.0
12	35.23467957841098	36.0	36.0	36.0	36.0	36.0
13	35.265389094021515	36.0	36.0	36.0	36.0	36.0
14	35.22383630084497	36.0	36.0	36.0	36.0	36.0
15	35.21686754182549	36.0	36.0	36.0	36.0	36.0
16	35.22413587189108	36.0	36.0	36.0	36.0	36.0
17	35.20225424724066	36.0	36.0	36.0	36.0	36.0
18	35.193025269464655	36.0	36.0	36.0	36.0	36.0
19	35.17597659165779	36.0	36.0	36.0	36.0	36.0
20	35.15773361067707	36.0	36.0	36.0	36.0	36.0
21	35.138016660529274	36.0	36.0	36.0	36.0	36.0
22	35.1260188898952	36.0	36.0	36.0	36.0	36.0
23	35.098607641549805	36.0	36.0	36.0	36.0	36.0
24	35.08657702757845	36.0	36.0	36.0	36.0	36.0
25	35.07231207141933	36.0	36.0	36.0	36.0	36.0
26	35.03089264209719	36.0	36.0	36.0	36.0	36.0
27	35.012633737074154	36.0	36.0	36.0	36.0	36.0
28	34.9865780228311	36.0	36.0	36.0	36.0	36.0
29	34.96178727469968	36.0	36.0	36.0	36.0	36.0
30	34.926839973327226	36.0	36.0	36.0	36.0	36.0
31	34.91114981538063	36.0	36.0	36.0	36.0	36.0
32	34.883714680971764	36.0	36.0	36.0	36.0	36.0
33	34.86066861072683	36.0	36.0	36.0	32.0	36.0
34	34.84337111975875	36.0	36.0	36.0	32.0	36.0
35	34.82448122455885	36.0	36.0	36.0	32.0	36.0
36	34.7871323785543	36.0	36.0	36.0	32.0	36.0
37	34.74346268300208	36.0	36.0	36.0	32.0	36.0
38	34.733574847975156	36.0	36.0	36.0	32.0	36.0
39	34.66950545896076	36.0	36.0	36.0	32.0	36.0
40	34.66264418722693	36.0	36.0	36.0	32.0	36.0
41	34.602261214009175	36.0	36.0	36.0	32.0	36.0
42	34.58253928759816	36.0	36.0	36.0	32.0	36.0
43	34.54735213033829	36.0	36.0	36.0	32.0	36.0
44	34.45604367168606	36.0	36.0	36.0	32.0	36.0
45	34.418765488619286	36.0	36.0	36.0	32.0	36.0
46	34.30938423718861	36.0	36.0	36.0	32.0	36.0
47	34.25287777302268	36.0	36.0	36.0	32.0	36.0
48	34.205039959393694	36.0	36.0	36.0	32.0	36.0
49	34.138991012868615	36.0	36.0	36.0	32.0	36.0
50	33.74585029409716	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	3.0
20	16.0
21	20.0
22	78.0
23	266.0
24	647.0
25	1360.0
26	2954.0
27	5458.0
28	9378.0
29	14798.0
30	21556.0
31	31715.0
32	47982.0
33	77964.0
34	166570.0
35	624002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.983975660844706	20.522523275910128	13.83184399366035	22.661657069584816
2	14.412501816430082	21.730674676874507	41.00444104713959	22.852382459555816
3	18.059808891016523	27.926773072762145	29.43707347076486	24.576344565456466
4	10.802671258098869	17.536252077589896	38.01039043761259	33.650686226698646
5	11.977467480119829	40.52250763856405	32.61492679916797	14.885098082148154
6	29.856215707291923	38.39569094986012	18.736445889552723	13.011647453295236
7	28.133105088726772	33.921992097694	19.812892502761827	18.1320103108174
8	28.171123739761338	33.576241328861336	18.755934193895122	19.496700737482207
9	28.459946057306645	14.897737790738178	20.052748390178845	36.58956776177632
10	16.09792897671007	29.17207835830922	30.718304406286418	24.011688258694285
11	34.168914278889694	24.32696039889726	22.22229963076127	19.281825691451775
12	24.660071459139903	27.431153398290153	28.938563054231313	18.970212088338627
13	28.576490142022553	22.908128228350765	26.274172198612618	22.241209431014063
14	23.065079570448958	21.976273176945966	27.96202115907123	26.996626093533845
15	24.55268369875693	29.188470993361666	24.752032803527175	21.50681250435423
16	23.005899892910175	29.036967884995878	25.796405922186704	22.16072629990724
17	20.612876578719508	28.580073051544137	26.890631686853705	23.91641868288265
18	20.718174308548225	28.029001662071916	29.52068632622391	21.732137703155946
19	23.74772335957483	26.0874628024324	29.454800601132597	20.710013236860178
20	26.01834453326539	25.46558011401637	28.18959202522373	20.326483327494508
21	26.736666102690165	24.795027717786162	26.935318530608992	21.532987648914677
22	24.713616051434656	26.960000796202117	27.287837017426874	21.038546134936354
23	23.15325895478567	27.25469510435224	27.856822954507	21.735222986355083
24	22.6331980775566	26.917093881840792	28.31264686299157	22.137061177611038
25	22.556373766256257	26.990138012096338	28.815138236028847	21.638349985618557
26	21.515194039625026	27.220485504628428	29.027567530447296	22.236752925299246
27	22.131377591643044	27.907835440619127	28.284539316538744	21.67624765119908
28	21.500166707306757	27.39523565446303	28.37617130544262	22.728426332787595
29	21.80806277282472	27.23887950030156	28.58108256051658	22.371975166357142
30	22.499348101731957	27.01207653982352	28.695267915259198	21.793307443185324
31	22.553885627215063	26.94415720261513	28.389965036670194	22.11199213349961
32	21.56900213582068	27.722076583005396	28.640598905615832	22.06832237555809
33	21.788689738636442	27.433967409424454	28.99900375111095	21.778339100828152
34	21.742475362422695	27.465698481039368	29.271193491817993	21.520632664719944
35	22.479671964728247	27.64911372751973	28.337928083043877	21.533286224708142
36	21.81406870633947	27.508511906716866	28.632650887915528	22.044768499028137
37	22.293870352603022	27.380080337912887	27.569479555235297	22.756569754248794
38	21.700787244842104	27.684843297471062	28.59798759915205	22.01638185853479
39	22.098180073768418	27.654598185050048	28.148743090431005	22.098478650750522
40	22.965500493646292	27.168162202617918	28.658356954043125	21.207980349692665
41	22.908486877004826	27.242941867883275	28.303092371036755	21.545478884075152
42	21.927359010751232	28.10946709637094	28.171275238324633	21.7918986545532
43	21.637987405609252	27.97250612849384	28.53295353093807	21.856552934958838
44	21.726143969742964	27.792281470053993	28.33641046057379	22.145164099629252
45	22.49526996294597	27.87820639781677	27.828940362219818	21.797583277017445
46	22.78964036681908	27.577417576183432	27.31944192860683	22.313500128390658
47	22.58498580749953	27.95663822858485	27.278264573687544	22.180111390228074
48	22.04642200611303	28.00110277395423	27.605875754798014	22.346599465134727
49	22.58177851473934	27.558259664824043	27.258277381096935	22.60168443933968
50	22.34208572446589	28.409043407640077	26.7958127063938	22.453058161500227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	54.0
2	57.0
3	140.5
4	224.0
5	259.5
6	295.0
7	272.0
8	249.0
9	381.0
10	513.0
11	814.0
12	1115.0
13	2210.5
14	3306.0
15	4621.5
16	5937.0
17	6369.5
18	6802.0
19	6579.5
20	6357.0
21	6850.0
22	7343.0
23	7279.5
24	7216.0
25	8812.5
26	10409.0
27	13519.0
28	16629.0
29	20532.0
30	24435.0
31	27428.0
32	30421.0
33	36215.5
34	42010.0
35	47841.5
36	53673.0
37	59261.0
38	64849.0
39	66304.0
40	67759.0
41	69743.0
42	71727.0
43	71126.5
44	70526.0
45	71478.5
46	72431.0
47	72960.0
48	73489.0
49	70993.5
50	68498.0
51	66415.0
52	64332.0
53	59843.5
54	55355.0
55	51011.0
56	46667.0
57	41534.5
58	36402.0
59	31369.5
60	26337.0
61	23142.5
62	19948.0
63	16494.0
64	13040.0
65	11898.5
66	10757.0
67	9436.0
68	8115.0
69	7099.5
70	6084.0
71	5303.0
72	4522.0
73	3784.5
74	3047.0
75	2365.0
76	1683.0
77	1300.5
78	918.0
79	799.5
80	681.0
81	456.5
82	232.0
83	188.5
84	145.0
85	124.0
86	103.0
87	70.5
88	38.0
89	26.0
90	14.0
91	12.5
92	11.0
93	6.0
94	1.0
95	4.0
96	7.0
97	6.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029658528817540334
2	0.005175313753396299
3	2.9857579346517115E-4
4	0.0
5	0.0
6	9.952526448839038E-5
7	0.0
8	0.0
9	0.0
10	9.952526448839038E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.971515869303423E-4
17	0.0
18	0.0
19	0.0
20	1.9905052897678077E-4
21	0.0
22	0.0
23	0.0
24	6.966768514187326E-4
25	2.9857579346517115E-4
26	9.952526448839038E-5
27	1.9905052897678077E-4
28	0.0014928789673258557
29	3.9810105795356153E-4
30	0.0011943031738606846
31	2.9857579346517115E-4
32	3.9810105795356153E-4
33	9.952526448839038E-5
34	3.9810105795356153E-4
35	0.0
36	9.952526448839038E-5
37	0.0013933537028374653
38	0.0
39	3.9810105795356153E-4
40	1.9905052897678077E-4
41	6.966768514187326E-4
42	0.004777212695442738
43	0.003682434786070444
44	0.004478636901977567
45	0.002090030554256198
46	0.002388606347721369
47	0.0013933537028374653
48	0.0030852831991401014
49	0.004379111637489177
50	0.0015924042318142461
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1004770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.83215211085879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.81885926361277	26.058613689933267
2	14.940814162138519	12.201311930570267
3	7.0794266538797865	8.672046779666626
4	4.077950477034517	6.660459767152904
5	2.534909892165382	5.1752913152108775
6	1.7292031204607963	4.236425090713614
7	1.2037484322559089	3.4406147362356747
8	0.8973878750021529	2.9313822571622596
9	0.6161343273984917	2.2642281519351193
>10	2.921386111052436	19.64475855178027
>50	0.11621808696930626	3.205797434170505
>100	0.06127618747172861	4.628272975128573
>500	0.001953025860496299	0.47399176756641687
>1k	7.323846976861121E-4	0.40680555277361663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1736	0.17277585915184568	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1260	0.12540183325537188	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1085	0.10798491196990355	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	9.952526448839038E-5	0.0
15	0.0	0.0	0.0	1.9905052897678077E-4	0.0
16	0.0	0.0	0.0	2.9857579346517115E-4	0.0
17	0.0	0.0	0.0	3.9810105795356153E-4	0.0
18	0.0	0.0	0.0	3.9810105795356153E-4	0.0
19	0.0	0.0	0.0	4.976263224419519E-4	0.0
20	0.0	0.0	0.0	6.966768514187326E-4	0.0
21	0.0	0.0	0.0	7.962021159071231E-4	0.0
22	0.0	0.0	0.0	0.0011943031738606846	0.0
23	0.0	0.0	0.0	0.0015924042318142461	0.0
24	0.0	0.0	0.0	0.0024881316122097596	0.0
25	0.0	0.0	0.0	0.0032843337281168823	0.0
26	0.0	0.0	0.0	0.004279586373000787	0.0
27	0.0	0.0	0.0	0.005075788488907909	0.0
28	0.0	0.0	0.0	0.0064691421917453746	0.0
29	0.0	0.0	0.0	0.00776297063009445	0.0
30	0.0	0.0	0.0	0.011544930680653284	0.0
31	0.0	0.0	0.0	0.020203628691143248	0.0
32	0.0	0.0	0.0	0.030554256197935847	0.0
33	0.0	0.0	0.0	0.040606307911263274	0.0
34	0.0	0.0	0.0	0.05016073330214875	0.0
35	9.952526448839038E-5	0.0	0.0	0.06688097773619833	0.0
36	9.952526448839038E-5	0.0	0.0	0.08947321277506295	0.0
37	9.952526448839038E-5	0.0	0.0	0.11445405416164893	0.0
38	9.952526448839038E-5	0.0	0.0	0.14719786617832936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATTCG	30	0.0057449704	29.332903	10
GTATCAA	5325	0.0	26.322222	1
GGTATCA	1965	0.0	26.315285	1
TATACCG	45	0.0013981697	24.444084	5
GTATTAG	270	0.0	22.004059	1
GTACCGT	70	3.2150227E-5	21.999676	6
TACCGTG	95	6.8649024E-7	20.841799	7
CTATTAC	130	1.6189006E-9	20.311436	1
TTTCGCG	55	0.0044834763	19.999704	25
CGATATT	55	0.0044834763	19.999704	27
GATATAC	190	0.0	19.68784	1
GGTCGCG	80	8.987661E-5	19.249718	7
GCGGTAT	265	0.0	19.095959	44
TATCAAC	7640	0.0	18.977106	2
CGTCTTA	320	0.0	18.562227	15
ATACTGC	155	7.203198E-10	18.451342	6
TACGCCG	60	0.0074118813	18.333065	16
CTATACT	180	1.4551915E-11	18.333063	4
CGCTCTA	85	1.430689E-4	18.11738	15
TTCGACT	85	1.430689E-4	18.11738	22
>>END_MODULE