FastQCFastQC Report
Thu 2 Feb 2017
SRR4061979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743046
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31030.17802169305916193No Hit
TATCAACGCAGAGTACTTTTTTTTT18760.1076276816561353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA350.002170231416.28583715
GCGTAAC1257.2759576E-1213.68246211
CGGTCCA3300.012.95650410
GGTATCA7050.012.9310681
GTATCAA19250.012.7767771
TCGCGTA1352.7284841E-1112.6682199
ATCGTGC604.0882398E-412.6682188
GTAGGAC3100.012.2560453
CGCGTAA1405.2750693E-1112.21613210
CGCCGGT3500.012.2157827
TAAGCTG951.038823E-612.0011245
GTATTAG1201.0073563E-811.8703161
ACGGTAT2200.011.6605199
GTCTTAG1354.7566573E-911.2548191
ATAAGGC1104.9794835E-711.2254233
TAAACCG600.005872041511.08437255
GCCGGTC4100.010.8915778
ACCGTCC1053.461304E-610.8584738
ATCCGCT700.001490035310.8584727
CTAACCT700.00149253710.856294
Produced by FastQC (version 0.11.5)