##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061979_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1743046
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2539594480008	32.0	32.0	32.0	32.0	32.0
2	30.787180602233104	32.0	32.0	32.0	32.0	32.0
3	30.809120929682866	32.0	32.0	32.0	32.0	32.0
4	30.818233712707524	32.0	32.0	32.0	32.0	32.0
5	30.731981829509948	32.0	32.0	32.0	32.0	32.0
6	34.39237174463554	36.0	36.0	36.0	32.0	36.0
7	34.32227491414455	36.0	36.0	36.0	32.0	36.0
8	34.28412445798906	36.0	36.0	36.0	32.0	36.0
9	34.41846457293726	36.0	36.0	36.0	32.0	36.0
10	34.10465185657751	36.0	36.0	36.0	32.0	36.0
11	34.400905082252564	36.0	36.0	36.0	32.0	36.0
12	34.21751692152703	36.0	36.0	36.0	32.0	36.0
13	34.312888472249156	36.0	36.0	36.0	32.0	36.0
14	34.21264958010288	36.0	36.0	36.0	32.0	36.0
15	34.161183353738224	36.0	36.0	36.0	32.0	36.0
16	34.1710844120006	36.0	36.0	36.0	32.0	36.0
17	34.1006863846393	36.0	36.0	36.0	32.0	36.0
18	34.1068130158355	36.0	36.0	36.0	32.0	36.0
19	34.1112041793504	36.0	36.0	36.0	32.0	36.0
20	34.092642993931314	36.0	36.0	36.0	32.0	36.0
21	34.0822542835932	36.0	36.0	36.0	32.0	36.0
22	34.04904632465236	36.0	36.0	36.0	32.0	36.0
23	34.01680793277974	36.0	36.0	36.0	32.0	36.0
24	33.998351735984016	36.0	36.0	36.0	32.0	36.0
25	33.633309161089265	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	24.0
5	118.0
6	395.0
7	84.0
8	309.0
9	265.0
10	141.0
11	30.0
12	65.0
13	92.0
14	298.0
15	458.0
16	707.0
17	861.0
18	1194.0
19	1657.0
20	2392.0
21	3507.0
22	5127.0
23	7667.0
24	10733.0
25	15072.0
26	20530.0
27	26587.0
28	34676.0
29	45267.0
30	59243.0
31	80706.0
32	114220.0
33	162145.0
34	357847.0
35	790629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42947412748053	17.06869715730164	11.817544995000077	25.68428372021776
2	16.815027979819188	19.110669737160666	37.162041993250874	26.912260289769264
3	19.385165793765946	22.625598165043925	28.040451188986594	29.948784852203538
4	13.21389862853202	15.222244599016454	34.53404916124316	37.02980761120836
5	15.22133878774731	36.05818730264767	33.012806165140645	15.707667744464379
6	35.4406835220237	34.27759844171859	16.23462399801598	14.047094038241733
7	31.161314778333608	29.7777305542952	19.768943157604543	19.292011509766645
8	28.60529911555049	32.83996225337907	18.679523032503333	19.8752155985671
9	27.13378628016837	14.54337306766171	18.27041937025532	40.052421281914604
10	16.625475820341272	26.522538918743155	30.584864058717347	26.267121202198222
11	37.376279292369546	21.209321619545502	21.652696647482667	19.76170244060228
12	24.784677890073123	23.26495649895901	28.197142673303276	23.753222937664596
13	29.530923091240613	19.366843258434066	24.839407193960263	26.262826456365058
14	23.888837511997778	19.266353312306464	24.529006339653073	32.315802836042685
15	25.332915871958082	26.88771054990004	21.794126433347888	25.985247144793988
16	26.04421799967833	25.67556234634561	23.114043149599063	25.166176504376995
17	24.38991325546756	25.93857400283423	24.609919585675808	25.0615931560224
18	25.078573365007745	25.095689492765278	25.383676957786346	24.442060184440635
19	25.875368840831904	24.861681098694856	24.761638945456927	24.501311115016318
20	26.017579585353335	24.42684877950174	24.41392768089825	25.14164395424668
21	26.616863282647508	24.087778607897985	24.343897171268093	24.95146093818642
22	26.2913839583642	24.004849545771464	24.51507413878063	25.188692357083703
23	24.804341410028055	24.299447504929507	25.072837247653535	25.823373837388907
24	25.11779241086682	24.591207934255614	24.89228562355469	25.39871403132288
25	25.26097579883373	24.301829802261913	24.838625394428192	25.59856900447617
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	782.0
1	782.0
2	680.5
3	579.0
4	579.0
5	579.0
6	1164.5
7	1750.0
8	1750.0
9	1750.0
10	2044.0
11	2338.0
12	2338.0
13	2338.0
14	3123.0
15	3908.0
16	3908.0
17	3908.0
18	6383.0
19	8858.0
20	8858.0
21	8858.0
22	14395.0
23	19932.0
24	19932.0
25	19932.0
26	29759.0
27	39586.0
28	39586.0
29	39586.0
30	51656.5
31	63727.0
32	63727.0
33	63727.0
34	80293.5
35	96860.0
36	96860.0
37	96860.0
38	116403.0
39	135946.0
40	135946.0
41	135946.0
42	158339.5
43	180733.0
44	180733.0
45	180733.0
46	202836.5
47	224940.0
48	224940.0
49	224940.0
50	235349.0
51	245758.0
52	245758.0
53	245758.0
54	232741.5
55	219725.0
56	219725.0
57	219725.0
58	202205.5
59	184686.0
60	184686.0
61	184686.0
62	161829.0
63	138972.0
64	138972.0
65	138972.0
66	112803.5
67	86635.0
68	86635.0
69	86635.0
70	65783.5
71	44932.0
72	44932.0
73	44932.0
74	33454.0
75	21976.0
76	21976.0
77	21976.0
78	17083.5
79	12191.0
80	12191.0
81	12191.0
82	8401.5
83	4612.0
84	4612.0
85	4612.0
86	3333.0
87	2054.0
88	2054.0
89	2054.0
90	1356.0
91	658.0
92	658.0
93	658.0
94	413.5
95	169.0
96	169.0
97	169.0
98	454.0
99	739.0
100	739.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001090045816346786
2	2.2948332975721808E-4
3	4.015958270751317E-4
4	0.009695670682242466
5	0.03465198279333993
6	0.06465692815909621
7	0.10992251495370747
8	0.11261894407835478
9	0.12334728974450473
10	0.13585413121627313
11	0.14532031856875838
12	0.13757525618945224
13	0.13241188126991485
14	0.1204213772901002
15	0.1357393895513945
16	0.12197038976596143
17	0.125470010544759
18	0.11462692321373046
19	0.10212008174196206
20	0.09798938180633214
21	0.09552243601144203
22	0.10625078167759197
23	0.08559728199944236
24	0.0938013110382629
25	0.09185070273532656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1743046.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.16579337484814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31813952694773	36.27896844762299
2	15.151118509418943	15.201357610826957
3	5.233078854194565	7.87564657541435
4	2.3985582095354787	4.813023021484102
5	1.2941112041072254	3.2460057634659507
6	0.7792801725472756	2.3455924870273583
7	0.5458703357575801	1.9168812931151622
8	0.3939443397374409	1.5810024278767554
9	0.28969777290916204	1.3079626755223233
>10	1.4386909359632705	12.732037751718133
>50	0.08734356893779716	3.0084652177703877
>100	0.062000121964063945	6.261873112147928
>500	0.006211101280140573	2.0920612858923926
>1k	0.0019553466993035137	1.339122330115306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3103	0.17802169305916193	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1876	0.1076276816561353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.737083243930453E-5
7	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
8	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
9	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
10	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
11	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
12	0.0	0.0	0.0	5.737083243930453E-5	2.294833297572181E-4
13	0.0	0.0	0.0	5.737083243930453E-5	2.294833297572181E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	35	0.0021702314	16.285837	15
GCGTAAC	125	7.2759576E-12	13.682462	11
CGGTCCA	330	0.0	12.956504	10
GGTATCA	705	0.0	12.931068	1
GTATCAA	1925	0.0	12.776777	1
TCGCGTA	135	2.7284841E-11	12.668219	9
ATCGTGC	60	4.0882398E-4	12.668218	8
GTAGGAC	310	0.0	12.256045	3
CGCGTAA	140	5.2750693E-11	12.216132	10
CGCCGGT	350	0.0	12.215782	7
TAAGCTG	95	1.038823E-6	12.001124	5
GTATTAG	120	1.0073563E-8	11.870316	1
ACGGTAT	220	0.0	11.660519	9
GTCTTAG	135	4.7566573E-9	11.254819	1
ATAAGGC	110	4.9794835E-7	11.225423	3
TAAACCG	60	0.0058720415	11.0843725	5
GCCGGTC	410	0.0	10.891577	8
ACCGTCC	105	3.461304E-6	10.858473	8
ATCCGCT	70	0.0014900353	10.858472	7
CTAACCT	70	0.001492537	10.85629	4
>>END_MODULE