FastQCFastQC Report
Thu 2 Feb 2017
SRR4061979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743046
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9450.030.2732351
GTATCAA22300.024.5720731
GTATACG602.867913E-422.0063111
TAAGCGT856.1175015E-620.7052884
TTAACGG951.5981645E-518.52578535
ATGCGTA600.0074146118.33280827
ATTACAC2900.017.4477753
GTATAGG1702.4610927E-916.8283581
TGAACCG1053.7768143E-516.7614255
TCAACGC32300.016.7549364
TACACTA1451.2505552E-716.6891775
GTCCTAC4950.016.4491631
ATCAACG32850.016.4074443
GTATATA2550.016.396861
CAACGCA33400.016.3348625
TATCAAC34000.016.1769332
TAACGGC1105.6221514E-516.0004636
GTATAAG3200.015.8170381
AACGCAG34550.015.6638056
GCCTAAC850.002974952815.5338691
Produced by FastQC (version 0.11.5)