##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1743046
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280726957292003	32.0	32.0	32.0	32.0	32.0
2	31.459552989421965	32.0	32.0	32.0	32.0	32.0
3	31.578094898241353	32.0	32.0	32.0	32.0	32.0
4	31.658753125276096	32.0	32.0	32.0	32.0	32.0
5	31.629667834354343	32.0	32.0	32.0	32.0	32.0
6	35.281877816190736	36.0	36.0	36.0	36.0	36.0
7	35.28529080701255	36.0	36.0	36.0	36.0	36.0
8	35.23475398813342	36.0	36.0	36.0	36.0	36.0
9	35.31156492714478	36.0	36.0	36.0	36.0	36.0
10	35.2032918236237	36.0	36.0	36.0	36.0	36.0
11	35.31712702934977	36.0	36.0	36.0	36.0	36.0
12	35.24378415715936	36.0	36.0	36.0	36.0	36.0
13	35.27692097626798	36.0	36.0	36.0	36.0	36.0
14	35.23810673958117	36.0	36.0	36.0	36.0	36.0
15	35.21864712692608	36.0	36.0	36.0	36.0	36.0
16	35.2276319729944	36.0	36.0	36.0	36.0	36.0
17	35.20822456779683	36.0	36.0	36.0	36.0	36.0
18	35.20741908130939	36.0	36.0	36.0	36.0	36.0
19	35.198609216280005	36.0	36.0	36.0	36.0	36.0
20	35.20105837711684	36.0	36.0	36.0	36.0	36.0
21	35.19257093616577	36.0	36.0	36.0	36.0	36.0
22	35.17445265357311	36.0	36.0	36.0	36.0	36.0
23	35.14887731018	36.0	36.0	36.0	36.0	36.0
24	35.129722910353486	36.0	36.0	36.0	36.0	36.0
25	35.115367580660525	36.0	36.0	36.0	36.0	36.0
26	35.07026492703004	36.0	36.0	36.0	36.0	36.0
27	35.06379636567251	36.0	36.0	36.0	36.0	36.0
28	35.04351061303029	36.0	36.0	36.0	36.0	36.0
29	35.0256596785168	36.0	36.0	36.0	36.0	36.0
30	35.005280411417715	36.0	36.0	36.0	36.0	36.0
31	35.01086316712238	36.0	36.0	36.0	36.0	36.0
32	34.989988216031016	36.0	36.0	36.0	36.0	36.0
33	34.9652384389167	36.0	36.0	36.0	36.0	36.0
34	34.95397883934216	36.0	36.0	36.0	36.0	36.0
35	34.94845173334496	36.0	36.0	36.0	36.0	36.0
36	34.924862568170894	36.0	36.0	36.0	36.0	36.0
37	34.90818371976414	36.0	36.0	36.0	36.0	36.0
38	34.89425350793955	36.0	36.0	36.0	32.0	36.0
39	34.87424370900137	36.0	36.0	36.0	32.0	36.0
40	34.86043053367496	36.0	36.0	36.0	32.0	36.0
41	34.83917750879782	36.0	36.0	36.0	32.0	36.0
42	34.8061582998957	36.0	36.0	36.0	32.0	36.0
43	34.8093710665123	36.0	36.0	36.0	32.0	36.0
44	34.76483466299799	36.0	36.0	36.0	32.0	36.0
45	34.750938299964545	36.0	36.0	36.0	32.0	36.0
46	34.72686148271474	36.0	36.0	36.0	32.0	36.0
47	34.69513713350078	36.0	36.0	36.0	32.0	36.0
48	34.67781515806238	36.0	36.0	36.0	32.0	36.0
49	34.66393199032039	36.0	36.0	36.0	32.0	36.0
50	34.26429250863144	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	22.0
21	50.0
22	148.0
23	316.0
24	880.0
25	1929.0
26	4069.0
27	7580.0
28	13108.0
29	21049.0
30	31043.0
31	46308.0
32	70067.0
33	120105.0
34	277924.0
35	1148447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19615263432464	17.539954052910893	12.182635948805094	26.08125736395937
2	16.360206475509862	19.540473227548198	36.90012490196315	27.199195394978794
3	18.78575292922304	23.054160004451994	28.27429564111876	29.885791425206204
4	12.988018094770931	15.61359574767146	34.62280090301742	36.77558525454019
5	14.957321837748403	36.300189438488715	32.883526883398375	15.858961840364511
6	35.58852856098705	34.38236141183058	16.11865921762069	13.910450809561675
7	31.27014433354025	29.783780806702747	19.78037297925585	19.165701880501146
8	28.60819507918896	32.93183312431226	18.636054355421486	19.82391744107729
9	27.228311817358808	14.27036349012017	18.22929515342682	40.272029539094206
10	16.635992394922454	26.517946170095342	30.61588736040242	26.230174074579786
11	37.531998581793026	21.05767719268453	21.692428082792993	19.71789614272945
12	24.90829272434577	23.108225485730152	28.27567373437075	23.707808055553325
13	29.762152002873133	19.194788892547873	24.85166771272818	26.191391391850814
14	23.849686124175726	19.038568115815647	24.625798745414638	32.48594701459399
15	25.436333866117128	26.822068952856092	21.79380234371325	25.94779483731353
16	26.1042526996031	25.57861751405882	23.160936619710228	25.156193166627844
17	24.480421056013437	25.873901205131705	24.63629760775103	25.009380131103825
18	25.169158287637362	24.94523941477027	25.510572894973272	24.3750294026191
19	25.990822961643005	24.723157048064138	24.93221636147296	24.353803628819893
20	26.127353140456087	24.209041314528672	24.51282039513655	25.15078514987869
21	26.74152030411131	24.025240871439994	24.34393584564033	24.889302978808363
22	26.390927147074716	23.831556941124905	24.68580863614615	25.09170727565423
23	24.967399005762903	24.041203755496845	25.23245240369583	25.758944835044417
24	25.231479755023596	24.42386081668364	24.972160468151632	25.372498960141133
25	25.44846831175805	24.017465996268598	24.99133699436159	25.542728697611768
26	24.95235330497671	24.92464306572777	25.191246547694313	24.931757081601205
27	25.541338385040856	24.493055527666876	24.645891863702573	25.31971422358969
28	25.243672042588177	24.29973511476352	25.063180607899284	25.393412234749018
29	25.09704298576856	24.525570812407274	25.08086437389346	25.296521827930707
30	25.085813062632777	24.6035502483315	25.241920261980123	25.068716427055598
31	25.628584968118957	24.25650099079655	24.64255020819923	25.472363832885264
32	25.517227926808417	24.36567793547143	24.426319044521016	25.690775093199132
33	25.012851081211952	24.40179364376344	24.898568251862834	25.686787023161777
34	25.753531069799834	24.339037777383826	24.925775676452517	24.98165547636382
35	25.880513698728375	24.269826653930334	25.135323528652442	24.714336118688845
36	25.036258490912427	24.828747475674685	24.748083807600512	25.386910225812375
37	25.82525296742247	24.42979418948016	24.70127841992965	25.04367442316773
38	25.361694413113593	24.338944583218115	24.845643775322053	25.45371722834624
39	25.839486411593604	24.197059144134066	24.464065449246426	25.4993889950259
40	26.01585157009716	24.32346841303581	24.768551586447852	24.89212843041918
41	25.10011336619576	24.773669209374052	25.049741139536806	25.076476284893378
42	25.984379365799725	24.741257786363473	24.696735593829086	24.577627254007712
43	25.59205351359384	24.066544307697427	24.856235860969765	25.485166317738972
44	25.169151223172896	24.57436084561555	24.656060613884257	25.600427317327295
45	25.30421651839757	24.71919137212694	24.649197189676205	25.32739491979929
46	25.257473191290636	24.517311274775576	24.77049587578177	25.45471965815202
47	25.445491159022843	24.561852417069225	24.921515530516	25.07114089339193
48	25.919075144508668	24.940747859263617	24.06024182790918	25.079935168318535
49	25.471634245218826	24.929632090291236	24.182177440730886	25.416556223759052
50	25.425066838016775	25.10722768528187	24.271781161432457	25.195924315268904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	381.0
1	223.5
2	66.0
3	202.5
4	339.0
5	338.0
6	337.0
7	260.0
8	183.0
9	252.5
10	322.0
11	561.0
12	800.0
13	1544.0
14	2288.0
15	2827.0
16	3366.0
17	3225.0
18	3084.0
19	2876.5
20	2669.0
21	3062.5
22	3456.0
23	4108.5
24	4761.0
25	6157.0
26	7553.0
27	9635.5
28	11718.0
29	14030.0
30	16342.0
31	19313.0
32	22284.0
33	26488.0
34	30692.0
35	35998.5
36	41305.0
37	48551.5
38	55798.0
39	62824.0
40	69850.0
41	77015.5
42	84181.0
43	89232.0
44	94283.0
45	103856.0
46	113429.0
47	121198.5
48	128968.0
49	134379.0
50	139790.0
51	138908.0
52	138026.0
53	137647.5
54	137269.0
55	136792.0
56	136315.0
57	131272.5
58	126230.0
59	117022.5
60	107815.0
61	94204.5
62	80594.0
63	69495.0
64	58396.0
65	49179.0
66	39962.0
67	33948.0
68	27934.0
69	24068.0
70	20202.0
71	15902.5
72	11603.0
73	10050.5
74	8498.0
75	6394.5
76	4291.0
77	3633.5
78	2976.0
79	2550.5
80	2125.0
81	1639.5
82	1154.0
83	967.5
84	781.0
85	590.0
86	399.0
87	259.0
88	119.0
89	80.5
90	42.0
91	30.5
92	19.0
93	17.5
94	16.0
95	16.0
96	16.0
97	13.5
98	11.0
99	9.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03482409529065785
2	0.0045322957627050575
3	4.015958270751317E-4
4	5.737083243930452E-5
5	0.0
6	2.2948332975721808E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.884499892716543E-4
17	0.0
18	2.2948332975721808E-4
19	0.0
20	1.7211249731791358E-4
21	0.0
22	0.0
23	5.737083243930452E-5
24	0.0012047874812253952
25	1.1474166487860904E-4
26	4.5896665951443617E-4
27	2.8685416219652267E-4
28	0.0015490124758612224
29	2.2948332975721808E-4
30	7.458208217109588E-4
31	2.2948332975721808E-4
32	2.2948332975721808E-4
33	1.1474166487860904E-4
34	0.001090045816346786
35	5.737083243930452E-5
36	3.4422499463582717E-4
37	0.0020079791353756586
38	0.0
39	9.179333190288723E-4
40	5.737083243930452E-5
41	0.0012621583136646995
42	0.00545022908173393
43	0.004991262422219494
44	0.0045322957627050575
45	0.002524316627329399
46	0.002811170789525922
47	0.0014916416434219177
48	0.0040733291031906215
49	0.0036717332761154894
50	0.0014916416434219177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1743046.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.109782205584594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.68440674736176	36.63774416394199
2	15.646760476452586	15.994050403488815
3	5.478732233827145	8.400504337008643
4	2.385960805995758	4.877837485820171
5	1.2852936719552108	3.284553982192346
6	0.7837593055053145	2.4034660443586113
7	0.5066585138287413	1.8126644406074495
8	0.38181842872992694	1.561172538757207
9	0.28803153504780565	1.3249106122170198
>10	1.4199726064675837	12.669450968614079
>50	0.08000412683905696	2.834358027171505
>100	0.05181417785861845	5.3787505243017435
>500	0.0055430189400012065	1.9892460199887747
>1k	0.0012443511906125157	0.831290451531669
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.737083243930453E-5
2	0.0	0.0	0.0	0.0	5.737083243930453E-5
3	0.0	0.0	0.0	0.0	5.737083243930453E-5
4	0.0	0.0	0.0	0.0	5.737083243930453E-5
5	0.0	0.0	0.0	0.0	5.737083243930453E-5
6	0.0	0.0	0.0	0.0	5.737083243930453E-5
7	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
8	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
9	0.0	0.0	0.0	5.737083243930453E-5	5.737083243930453E-5
10	0.0	0.0	0.0	1.1474166487860906E-4	5.737083243930453E-5
11	0.0	0.0	0.0	1.1474166487860906E-4	5.737083243930453E-5
12	0.0	0.0	0.0	1.1474166487860906E-4	1.1474166487860906E-4
13	0.0	0.0	0.0	1.1474166487860906E-4	1.7211249731791358E-4
14	0.0	0.0	0.0	1.1474166487860906E-4	1.7211249731791358E-4
15	0.0	0.0	0.0	1.1474166487860906E-4	2.868541621965226E-4
16	0.0	0.0	0.0	1.7211249731791358E-4	3.4422499463582717E-4
17	0.0	0.0	0.0	1.7211249731791358E-4	3.4422499463582717E-4
18	0.0	0.0	0.0	2.294833297572181E-4	4.0159582707513167E-4
19	0.0	0.0	0.0	3.4422499463582717E-4	4.0159582707513167E-4
20	0.0	0.0	0.0	4.0159582707513167E-4	4.0159582707513167E-4
21	0.0	0.0	0.0	4.0159582707513167E-4	4.0159582707513167E-4
22	0.0	0.0	0.0	5.737083243930452E-4	4.0159582707513167E-4
23	0.0	0.0	0.0	6.884499892716543E-4	4.589666595144362E-4
24	0.0	0.0	0.0	0.0010326749839074814	4.589666595144362E-4
25	0.0	0.0	0.0	0.0013768999785433087	4.589666595144362E-4
26	0.0	0.0	0.0	0.001721124973179136	4.589666595144362E-4
27	0.0	0.0	0.0	0.002065349967814963	4.589666595144362E-4
28	0.0	0.0	0.0	0.0023522041300114856	4.589666595144362E-4
29	0.0	0.0	0.0	0.003499620778797576	4.589666595144362E-4
30	0.0	0.0	0.0	0.005564970746612539	4.589666595144362E-4
31	0.0	0.0	0.0	0.009580929017363855	4.589666595144362E-4
32	0.0	0.0	0.0	0.014916416434219178	5.737083243930452E-4
33	0.0	0.0	0.0	0.01939134136448493	5.737083243930452E-4
34	0.0	0.0	0.0	0.02380889546231138	6.310791568323498E-4
35	0.0	0.0	0.0	0.02937386620892392	6.310791568323498E-4
36	0.0	0.0	0.0	0.03740578275042655	6.310791568323498E-4
37	0.0	0.0	0.0	0.04933891589780189	6.310791568323498E-4
38	0.0	0.0	0.0	0.06482904065641412	6.310791568323498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	945	0.0	30.273235	1
GTATCAA	2230	0.0	24.572073	1
GTATACG	60	2.867913E-4	22.006311	1
TAAGCGT	85	6.1175015E-6	20.705288	4
TTAACGG	95	1.5981645E-5	18.525785	35
ATGCGTA	60	0.00741461	18.332808	27
ATTACAC	290	0.0	17.447775	3
GTATAGG	170	2.4610927E-9	16.828358	1
TGAACCG	105	3.7768143E-5	16.761425	5
TCAACGC	3230	0.0	16.754936	4
TACACTA	145	1.2505552E-7	16.689177	5
GTCCTAC	495	0.0	16.449163	1
ATCAACG	3285	0.0	16.407444	3
GTATATA	255	0.0	16.39686	1
CAACGCA	3340	0.0	16.334862	5
TATCAAC	3400	0.0	16.176933	2
TAACGGC	110	5.6221514E-5	16.00046	36
GTATAAG	320	0.0	15.817038	1
AACGCAG	3455	0.0	15.663805	6
GCCTAAC	85	0.0029749528	15.533869	1
>>END_MODULE