FastQCFastQC Report
Thu 2 Feb 2017
SRR4061978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151436
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46760.1483768034635639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.014.167861
TCGCGTA2550.014.1591249
GTATCAA34550.012.7797361
CGTTATT2200.012.5187582
CGTCGTA5350.012.43169610
AACCGTC853.9419974E-612.2954967
GCGTTAT2350.012.121891
CGCATCG4400.012.09207213
CCGTCGT5450.011.8551049
GCGTAAC3100.011.34088211
CGCGTAA3050.011.21472810
GTCCTAG3400.011.1711541
GTCGCGT3150.011.1599818
CGAGCCG6050.011.14947815
ATACCGT5900.011.1114156
GTCGTAG5900.010.9512511
AACCGCG2100.010.858367
AAGACGG6150.010.8135335
GCATCGC5100.010.80496114
ACCGTCG6000.010.7678748
Produced by FastQC (version 0.11.5)