##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061978_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3151436
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216265854677044	32.0	32.0	32.0	32.0	32.0
2	30.795731850496093	32.0	32.0	32.0	32.0	32.0
3	30.817902695786938	32.0	32.0	32.0	32.0	32.0
4	30.826130373582075	32.0	32.0	32.0	32.0	32.0
5	30.690469677949988	32.0	32.0	32.0	32.0	32.0
6	34.3548874227495	36.0	36.0	36.0	32.0	36.0
7	34.288822619275784	36.0	36.0	36.0	32.0	36.0
8	34.24375871824781	36.0	36.0	36.0	32.0	36.0
9	34.37836878172363	36.0	36.0	36.0	32.0	36.0
10	34.02666181385248	36.0	36.0	36.0	32.0	36.0
11	34.37162074685953	36.0	36.0	36.0	32.0	36.0
12	34.16813509777765	36.0	36.0	36.0	32.0	36.0
13	34.275113948054155	36.0	36.0	36.0	32.0	36.0
14	34.16421656666992	36.0	36.0	36.0	32.0	36.0
15	34.09621804155312	36.0	36.0	36.0	32.0	36.0
16	34.10053258260679	36.0	36.0	36.0	32.0	36.0
17	34.03134126791723	36.0	36.0	36.0	32.0	36.0
18	34.03841772449131	36.0	36.0	36.0	32.0	36.0
19	34.04299532022862	36.0	36.0	36.0	32.0	36.0
20	34.02977119002258	36.0	36.0	36.0	32.0	36.0
21	34.00639454521685	36.0	36.0	36.0	32.0	36.0
22	33.9745382739805	36.0	36.0	36.0	32.0	36.0
23	33.94173862328158	36.0	36.0	36.0	32.0	36.0
24	33.91671574482236	36.0	36.0	36.0	32.0	36.0
25	33.55363586631618	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	35.0
5	235.0
6	708.0
7	148.0
8	552.0
9	395.0
10	219.0
11	64.0
12	138.0
13	124.0
14	353.0
15	554.0
16	915.0
17	1207.0
18	1672.0
19	2182.0
20	3522.0
21	5213.0
22	7893.0
23	11712.0
24	17059.0
25	24756.0
26	35279.0
27	47087.0
28	64193.0
29	88710.0
30	118778.0
31	164447.0
32	235549.0
33	329792.0
34	687668.0
35	1300275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.412296495337635	17.66245626549707	12.214690477444643	25.710556761720653
2	16.553411166807187	19.701316450410275	37.41835846531843	26.326913917464108
3	18.81206529503059	23.346340172474687	28.29198529297437	29.549609239520358
4	12.744523082801305	15.886725223364035	35.63044288183238	35.73830881200229
5	15.188625013530238	36.16470037783092	33.1988403831827	15.447834225456148
6	34.27766204265974	35.114098315474564	16.928539200548162	13.679700441317532
7	30.649725231091768	30.261427527715128	20.35977256122741	18.72907467996569
8	28.251267369047	32.67939557638251	19.29180443683199	19.777532617738498
9	27.134232349853992	14.673530435843412	18.88627254636348	39.305964667939115
10	16.35105280700344	26.586296049284886	31.049203861272034	26.01344728243964
11	36.63599368174006	21.47782587544156	22.307010110042075	19.579170332776307
12	24.4846389358779	23.761004642697824	28.60155409003504	23.15280233138924
13	28.90665334808798	19.785650947121873	25.297388421160655	26.01030728362949
14	23.600246900794613	19.74605941947816	25.181021931665153	31.472671748062076
15	25.189774920002378	27.193885769970972	22.05058906402066	25.565750246005997
16	25.743118420822725	25.99952664075369	23.540028242709393	24.717326695714192
17	24.021426256695705	26.093044091576278	25.04663959790821	24.83889005381981
18	24.560141755925926	25.40748793022498	25.903684497764573	24.12868581608452
19	25.401939167097048	25.166546921859684	25.37916541998665	24.05234849105662
20	25.715551603118897	24.543551793682806	24.83981515300694	24.90108145019136
21	26.49411728835006	24.383461837011854	24.54162998309062	24.58079089154747
22	25.798017645773296	24.419092198149023	24.9095031535721	24.873387002505577
23	24.44728567622216	24.567233505109296	25.275868891338444	25.7096119273301
24	24.550758607985312	25.124740752033485	25.27128532997939	25.053215310001807
25	24.76624887888389	24.63778104125961	25.317882468919993	25.278087610936506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	274.0
1	274.0
2	617.5
3	961.0
4	961.0
5	961.0
6	2080.0
7	3199.0
8	3199.0
9	3199.0
10	3792.0
11	4385.0
12	4385.0
13	4385.0
14	6447.5
15	8510.0
16	8510.0
17	8510.0
18	14950.5
19	21391.0
20	21391.0
21	21391.0
22	33658.0
23	45925.0
24	45925.0
25	45925.0
26	66692.5
27	87460.0
28	87460.0
29	87460.0
30	110030.5
31	132601.0
32	132601.0
33	132601.0
34	164164.5
35	195728.0
36	195728.0
37	195728.0
38	228605.0
39	261482.0
40	261482.0
41	261482.0
42	300924.5
43	340367.0
44	340367.0
45	340367.0
46	373905.5
47	407444.0
48	407444.0
49	407444.0
50	418722.0
51	430000.0
52	430000.0
53	430000.0
54	400924.0
55	371848.0
56	371848.0
57	371848.0
58	340522.5
59	309197.0
60	309197.0
61	309197.0
62	271124.5
63	233052.0
64	233052.0
65	233052.0
66	189437.0
67	145822.0
68	145822.0
69	145822.0
70	110586.0
71	75350.0
72	75350.0
73	75350.0
74	56629.5
75	37909.0
76	37909.0
77	37909.0
78	30245.5
79	22582.0
80	22582.0
81	22582.0
82	15647.0
83	8712.0
84	8712.0
85	8712.0
86	6494.0
87	4276.0
88	4276.0
89	4276.0
90	2835.5
91	1395.0
92	1395.0
93	1395.0
94	891.0
95	387.0
96	387.0
97	387.0
98	783.0
99	1179.0
100	1179.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001078873250162783
2	2.2212096326880825E-4
3	5.394366250813915E-4
4	0.009582932986740013
5	0.03442874930666528
6	0.06355832706106042
7	0.10585650478067776
8	0.10722096212647186
9	0.11740679487065579
10	0.13273314133620356
11	0.13857174951355508
12	0.13098790519623435
13	0.12543488111451415
14	0.11464614861288631
15	0.13184465748312832
16	0.1180731577604622
17	0.12362618184218242
18	0.11074316597259155
19	0.09754283444118808
20	0.09157729999911152
21	0.09164076313147404
22	0.10344490575090212
23	0.08145493038729011
24	0.09221193132273668
25	0.0884676065133482
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3151436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.88116467856135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.14667576301069	32.184111823363416
2	16.02483257594026	14.704759647261788
3	6.026580680938769	8.295196220123643
4	2.713732252898743	4.980367855550821
5	1.4471944589549701	3.319948364660724
6	0.8366017678087256	2.303055808752461
7	0.5516316441109652	1.7716651613752517
8	0.38504789077984747	1.413315654880231
9	0.27428721696531955	1.1326155273729102
>10	1.3903711853419984	11.688848675985728
>50	0.10891445139384202	3.4796274595838597
>100	0.07905274569358318	7.461817289817151
>500	0.00965785205806589	3.156115757142391
>1k	0.0054195141041895505	4.108554754129461
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4676	0.1483768034635639	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.173156618125832E-5
2	0.0	0.0	0.0	0.0	3.173156618125832E-5
3	0.0	0.0	0.0	0.0	3.173156618125832E-5
4	0.0	0.0	0.0	0.0	3.173156618125832E-5
5	0.0	0.0	0.0	0.0	3.173156618125832E-5
6	0.0	0.0	0.0	0.0	3.173156618125832E-5
7	0.0	0.0	0.0	0.0	3.173156618125832E-5
8	0.0	0.0	0.0	3.173156618125832E-5	3.173156618125832E-5
9	0.0	0.0	0.0	6.346313236251664E-5	3.173156618125832E-5
10	0.0	0.0	0.0	1.5865783090629162E-4	3.173156618125832E-5
11	0.0	0.0	0.0	1.5865783090629162E-4	6.346313236251664E-5
12	0.0	0.0	0.0	1.5865783090629162E-4	2.2212096326880825E-4
13	0.0	0.0	0.0	1.5865783090629162E-4	2.855840956313249E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1260	0.0	14.16786	1
TCGCGTA	255	0.0	14.159124	9
GTATCAA	3455	0.0	12.779736	1
CGTTATT	220	0.0	12.518758	2
CGTCGTA	535	0.0	12.431696	10
AACCGTC	85	3.9419974E-6	12.295496	7
GCGTTAT	235	0.0	12.12189	1
CGCATCG	440	0.0	12.092072	13
CCGTCGT	545	0.0	11.855104	9
GCGTAAC	310	0.0	11.340882	11
CGCGTAA	305	0.0	11.214728	10
GTCCTAG	340	0.0	11.171154	1
GTCGCGT	315	0.0	11.159981	8
CGAGCCG	605	0.0	11.149478	15
ATACCGT	590	0.0	11.111415	6
GTCGTAG	590	0.0	10.95125	11
AACCGCG	210	0.0	10.85836	7
AAGACGG	615	0.0	10.813533	5
GCATCGC	510	0.0	10.804961	14
ACCGTCG	600	0.0	10.767874	8
>>END_MODULE