FastQCFastQC Report
Thu 2 Feb 2017
SRR4061978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151436
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA31620.1003352122651388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36900.027.4314291
GGTATCA17200.022.6444531
TTAACGG2050.018.2432335
CGTCGTA10400.018.19134910
TCAACGC57150.017.5528814
CAACGCA58150.017.2131965
AACGCAG59050.017.0626076
ATCAACG58600.017.0434723
ATACCGT10700.016.653336
TATCAAC60950.016.5670682
TACCGTC10500.016.3419977
CGCAATA6600.015.9999236
ACCGTCG10750.015.961958
CCGTCGT10900.015.74229059
CGTTCGG1552.6728048E-715.612082514
TAGGACG2403.6379788E-1215.5825144
GTCGTAG11100.015.26045811
ATACGAA6950.015.19513340
TAGTACT3250.014.8915244
TACGAAT7200.014.66752341
Produced by FastQC (version 0.11.5)