##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3151436
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89917612161567	32.0	32.0	32.0	32.0	32.0
2	31.24807262467015	32.0	32.0	32.0	32.0	32.0
3	31.4440359886731	32.0	32.0	32.0	32.0	32.0
4	31.57014040583404	32.0	32.0	32.0	32.0	32.0
5	31.489947439833777	32.0	32.0	32.0	32.0	32.0
6	35.155876241814845	36.0	36.0	36.0	36.0	36.0
7	35.15745932965162	36.0	36.0	36.0	36.0	36.0
8	35.095562150080156	36.0	36.0	36.0	36.0	36.0
9	35.23025281173408	36.0	36.0	36.0	36.0	36.0
10	35.04746724985054	36.0	36.0	36.0	36.0	36.0
11	35.245115242702056	36.0	36.0	36.0	36.0	36.0
12	35.129712296235745	36.0	36.0	36.0	36.0	36.0
13	35.19023962409518	36.0	36.0	36.0	36.0	36.0
14	35.14863033867735	36.0	36.0	36.0	36.0	36.0
15	35.102688742528805	36.0	36.0	36.0	36.0	36.0
16	35.125187057582636	36.0	36.0	36.0	36.0	36.0
17	35.107039457567915	36.0	36.0	36.0	36.0	36.0
18	35.10262496208078	36.0	36.0	36.0	36.0	36.0
19	35.097696415221506	36.0	36.0	36.0	36.0	36.0
20	35.10442699772421	36.0	36.0	36.0	36.0	36.0
21	35.09278087830437	36.0	36.0	36.0	36.0	36.0
22	35.07564519793517	36.0	36.0	36.0	36.0	36.0
23	35.04374989687241	36.0	36.0	36.0	36.0	36.0
24	35.02146450062765	36.0	36.0	36.0	36.0	36.0
25	35.007982075472896	36.0	36.0	36.0	36.0	36.0
26	34.96439876932294	36.0	36.0	36.0	36.0	36.0
27	34.94846476336502	36.0	36.0	36.0	32.0	36.0
28	34.92769581866806	36.0	36.0	36.0	32.0	36.0
29	34.90375340003732	36.0	36.0	36.0	32.0	36.0
30	34.88274583396267	36.0	36.0	36.0	32.0	36.0
31	34.88861966417849	36.0	36.0	36.0	32.0	36.0
32	34.858864339939	36.0	36.0	36.0	32.0	36.0
33	34.83748678380269	36.0	36.0	36.0	32.0	36.0
34	34.825963465543964	36.0	36.0	36.0	32.0	36.0
35	34.81399780925267	36.0	36.0	36.0	32.0	36.0
36	34.78833522241924	36.0	36.0	36.0	32.0	36.0
37	34.77964077328558	36.0	36.0	36.0	32.0	36.0
38	34.756702341408804	36.0	36.0	36.0	32.0	36.0
39	34.748831643733205	36.0	36.0	36.0	32.0	36.0
40	34.73553421360929	36.0	36.0	36.0	32.0	36.0
41	34.70333238561722	36.0	36.0	36.0	32.0	36.0
42	34.67444618897544	36.0	36.0	36.0	32.0	36.0
43	34.67221672913554	36.0	36.0	36.0	32.0	36.0
44	34.6211514369957	36.0	36.0	36.0	32.0	36.0
45	34.611470453469465	36.0	36.0	36.0	32.0	36.0
46	34.59239851293188	36.0	36.0	36.0	32.0	36.0
47	34.56742037598098	36.0	36.0	36.0	32.0	36.0
48	34.54397741220193	36.0	36.0	36.0	32.0	36.0
49	34.5290356523185	36.0	36.0	36.0	32.0	36.0
50	34.083964579956564	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	14.0
20	26.0
21	89.0
22	206.0
23	577.0
24	1463.0
25	3344.0
26	7202.0
27	13730.0
28	24284.0
29	39752.0
30	62081.0
31	95813.0
32	151558.0
33	269381.0
34	609483.0
35	1872428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64764696770786	18.62527329099492	12.710030953934757	26.01704878736247
2	15.973128592757604	20.191000236409995	36.9770618838862	26.8588092869462
3	18.071617057410165	23.828277212742922	28.683155699574925	29.416950030271995
4	12.542290271666804	16.359504911097613	35.838922852263444	35.25928196497214
5	14.908763785386657	36.60494346226402	32.900567519241235	15.585725233108091
6	34.50559555858487	35.29295290896698	16.72381536243252	13.477636170015625
7	30.619120934075767	30.33702731072438	20.385690840619958	18.658160914579895
8	28.254262501285126	32.89145011988185	19.166532336369833	19.68775504246318
9	27.23958220950703	14.458995835549254	18.72692321849468	39.57449873644903
10	16.279985555781906	26.69422872254345	31.0713789341614	25.95440678751324
11	36.87817236332897	21.358168149376983	22.28479334500209	19.47886614229196
12	24.51783885187578	23.664005869070483	28.586714120166175	23.231441158887566
13	29.03222530935104	19.710823891076956	25.34016238946309	25.916788410108914
14	23.745175215362142	19.58132736949124	25.123657913408366	31.54983950173825
15	25.22139113724664	27.226223220144725	21.970872960770897	25.581512681837744
16	25.83199160757679	25.938991195698186	23.464387731983166	24.76462946474186
17	24.067504464631362	26.140400756988242	25.00482319805955	24.787271580320844
18	24.774412148378214	25.340884607097784	25.811178597165163	24.073524647358838
19	25.549305142163764	25.127624359181024	25.334577633815186	23.988492864840026
20	25.81486765381579	24.45104320829388	24.824302095047585	24.909787042842744
21	26.62190398976974	24.370643849547903	24.492556565501115	24.514895595181244
22	25.83628542670706	24.389389471974045	24.962556751906114	24.811768349412773
23	24.50251250541023	24.41490165118378	25.36691209975389	25.7156737436521
24	24.643596154230597	25.049255714345097	25.285118316842837	25.02202981458147
25	24.90403547601482	24.431899854827577	25.43151114178507	25.232553527372538
26	24.704482798887106	25.25661415284011	25.51633801100709	24.522565037265693
27	25.177538788814203	24.96395280355801	24.832075912349467	25.02643249527832
28	24.750958705458075	24.75432230590677	25.43633989499855	25.058379093636606
29	24.698738598775915	24.958114066819487	25.35945746293095	24.98368987147365
30	24.760311812009064	24.983515189824416	25.535653201480237	24.720519796686283
31	25.306108331585442	24.678232046092283	24.786595997927282	25.229063624394993
32	25.088603056087504	24.848171376984624	24.751230796324826	25.311994770603047
33	24.669538383429717	24.676392425648714	25.167852638092292	25.486216552829276
34	25.192913893036952	24.70405273074939	25.492335801861138	24.61069757435253
35	25.718959329574194	24.520804840976954	25.25913632858734	24.501099500861514
36	24.647310567561934	25.104024303910577	24.928960753334916	25.31970437519257
37	25.581261040794494	24.907841990543194	24.88439195387673	24.626505014785582
38	24.909818888912863	24.611446972110492	25.238621377683064	25.24011276129358
39	25.472239648234652	24.690084054199076	24.7222600358759	25.11541626169037
40	25.55221703904224	24.799321451542838	25.102770707801813	24.545690801613105
41	24.645237487799086	25.22846959640846	25.309227252361172	24.817065663431283
42	25.750444975513258	25.14107683280884	24.9317661902385	24.17671200143941
43	25.276583564665845	24.343701091737145	25.186493989162596	25.193221354434414
44	24.845405021865584	25.044307125410825	24.91705801321931	25.193229839504284
45	25.123462686074603	25.149070701923016	24.848628701857645	24.878837910144743
46	24.896775118900592	25.02890804386153	24.87078643841476	25.203530398823116
47	25.04818478078067	24.857286103680575	25.304419291682134	24.790109823856625
48	25.689533384909375	25.250386026996818	24.362254207265877	24.697826380827927
49	24.965474916638424	25.337317680269805	24.67347140828069	25.02373599481108
50	25.211279717432717	25.3664492917572	24.496547714961427	24.925723275848664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	57.5
2	51.0
3	151.0
4	251.0
5	266.5
6	282.0
7	272.5
8	263.0
9	352.5
10	442.0
11	706.0
12	970.0
13	1776.0
14	2582.0
15	3434.0
16	4286.0
17	4540.5
18	4795.0
19	5102.0
20	5409.0
21	6735.5
22	8062.0
23	9931.0
24	11800.0
25	15663.0
26	19526.0
27	24625.0
28	29724.0
29	34993.0
30	40262.0
31	45895.5
32	51529.0
33	59024.0
34	66519.0
35	76639.0
36	86759.0
37	101802.0
38	116845.0
39	128858.0
40	140871.0
41	153189.5
42	165508.0
43	171792.0
44	178076.0
45	192588.0
46	207100.0
47	219840.5
48	232581.0
49	240171.5
50	247762.0
51	241503.5
52	235245.0
53	232878.0
54	230511.0
55	231883.0
56	233255.0
57	225350.5
58	217446.0
59	197178.5
60	176911.0
61	155005.0
62	133099.0
63	114874.5
64	96650.0
65	81115.5
66	65581.0
67	56473.0
68	47365.0
69	41907.0
70	36449.0
71	28374.5
72	20300.0
73	17681.5
74	15063.0
75	10977.0
76	6891.0
77	6208.0
78	5525.0
79	4618.5
80	3712.0
81	2919.0
82	2126.0
83	1824.0
84	1522.0
85	1180.5
86	839.0
87	576.5
88	314.0
89	208.5
90	103.0
91	80.0
92	57.0
93	60.0
94	63.0
95	52.0
96	41.0
97	37.5
98	34.0
99	24.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030081524739832888
2	0.00415683516974484
3	2.538525294500666E-4
4	6.346313236251664E-5
5	3.173156618125832E-5
6	2.538525294500666E-4
7	0.0
8	0.0
9	0.0
10	6.346313236251664E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.980944559876831E-4
17	0.0
18	9.519469854377496E-5
19	0.0
20	1.269262647250333E-4
21	3.173156618125832E-5
22	0.0
23	0.0
24	7.93289154531458E-4
25	3.4904722799384153E-4
26	3.8077879417509983E-4
27	3.8077879417509983E-4
28	0.0016183098752441745
29	6.346313236251665E-4
30	0.001586578309062916
31	6.028997574439081E-4
32	6.663628898064248E-4
33	3.4904722799384153E-4
34	9.519469854377497E-4
35	1.9038939708754992E-4
36	2.8558409563132493E-4
37	0.0017452361399692075
38	0.0
39	5.394366250813915E-4
40	9.519469854377496E-5
41	0.0010154101178002663
42	0.004410687699194907
43	0.004061640471201065
44	0.00434722456683239
45	0.0025067937283194076
46	0.0020308202356005326
47	8.250207207127164E-4
48	0.003236619750488349
49	0.0033000828828508655
50	0.0014279204781566244
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3151436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.331640721427625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.01617422235972	33.139804029816126
2	16.309323641220637	15.438940939914819
3	5.987386483188551	8.501784776478377
4	2.7326888056073826	5.173705790019032
5	1.447003882190827	3.4244533937183608
6	0.8334824410624183	2.3670054868790915
7	0.526955281854771	1.7459160653906056
8	0.3837304342720984	1.4530072839075487
9	0.2580082341277477	1.0990757736814083
>10	1.3206284846166567	11.33996403995588
>50	0.10167952717960531	3.3394951721409285
>100	0.07026011121591776	6.822872970498237
>500	0.00802518979460591	2.6827618772839346
>1k	0.004653261309016113	3.4712124003156903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3162	0.1003352122651388	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.173156618125832E-5
2	0.0	0.0	0.0	0.0	3.173156618125832E-5
3	0.0	0.0	0.0	0.0	3.173156618125832E-5
4	0.0	0.0	0.0	3.173156618125832E-5	3.173156618125832E-5
5	0.0	0.0	0.0	3.173156618125832E-5	3.173156618125832E-5
6	3.173156618125832E-5	0.0	0.0	3.173156618125832E-5	6.346313236251664E-5
7	3.173156618125832E-5	0.0	0.0	3.173156618125832E-5	6.346313236251664E-5
8	3.173156618125832E-5	0.0	0.0	3.173156618125832E-5	9.519469854377496E-5
9	3.173156618125832E-5	0.0	0.0	3.173156618125832E-5	9.519469854377496E-5
10	6.346313236251664E-5	0.0	0.0	9.519469854377496E-5	1.269262647250333E-4
11	6.346313236251664E-5	0.0	0.0	9.519469854377496E-5	1.269262647250333E-4
12	6.346313236251664E-5	0.0	0.0	9.519469854377496E-5	3.1731566181258323E-4
13	6.346313236251664E-5	0.0	0.0	9.519469854377496E-5	3.4904722799384153E-4
14	6.346313236251664E-5	0.0	0.0	9.519469854377496E-5	3.8077879417509983E-4
15	6.346313236251664E-5	0.0	0.0	9.519469854377496E-5	4.7597349271887485E-4
16	6.346313236251664E-5	0.0	0.0	1.5865783090629162E-4	5.711681912626498E-4
17	6.346313236251664E-5	0.0	0.0	1.9038939708754992E-4	5.711681912626498E-4
18	6.346313236251664E-5	0.0	0.0	2.538525294500666E-4	5.711681912626498E-4
19	6.346313236251664E-5	0.0	0.0	3.8077879417509983E-4	5.711681912626498E-4
20	6.346313236251664E-5	0.0	0.0	6.663628898064247E-4	6.663628898064247E-4
21	6.346313236251664E-5	0.0	0.0	7.615575883501997E-4	6.980944559876831E-4
22	6.346313236251664E-5	0.0	0.0	0.0010471416839815245	6.980944559876831E-4
23	6.346313236251664E-5	0.0	0.0	0.0013961889119753661	7.615575883501997E-4
24	6.346313236251664E-5	0.0	0.0	0.0018404308385129827	7.615575883501997E-4
25	6.346313236251664E-5	0.0	0.0	0.002538525294500666	7.615575883501997E-4
26	6.346313236251664E-5	0.0	0.0	0.003173156618125832	8.567522868939747E-4
27	6.346313236251664E-5	0.0	0.0	0.0038077879417509988	8.567522868939747E-4
28	6.346313236251664E-5	0.0	0.0	0.004664540228644974	8.567522868939747E-4
29	6.346313236251664E-5	0.0	0.0	0.005743413478807757	8.567522868939747E-4
30	6.346313236251664E-5	0.0	0.0	0.0073299917878706725	8.567522868939747E-4
31	6.346313236251664E-5	0.0	0.0	0.009868517082371337	8.88483853075233E-4
32	6.346313236251664E-5	0.0	0.0	0.013358989362309753	9.519469854377497E-4
33	6.346313236251664E-5	0.0	0.0	0.016310025017166777	9.83678551619008E-4
34	6.346313236251664E-5	0.0	0.0	0.020308202356005327	0.0010154101178002663
35	6.346313236251664E-5	0.0	0.0	0.02402079559921255	0.0010154101178002663
36	6.346313236251664E-5	0.0	0.0	0.028463214864588714	0.0010154101178002663
37	6.346313236251664E-5	0.0	0.0	0.03576147508627813	0.0010154101178002663
38	6.346313236251664E-5	0.0	0.0	0.046042502529005824	0.0010154101178002663
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3690	0.0	27.431429	1
GGTATCA	1720	0.0	22.644453	1
TTAACGG	205	0.0	18.24323	35
CGTCGTA	1040	0.0	18.191349	10
TCAACGC	5715	0.0	17.552881	4
CAACGCA	5815	0.0	17.213196	5
AACGCAG	5905	0.0	17.062607	6
ATCAACG	5860	0.0	17.043472	3
ATACCGT	1070	0.0	16.65333	6
TATCAAC	6095	0.0	16.567068	2
TACCGTC	1050	0.0	16.341997	7
CGCAATA	660	0.0	15.99992	36
ACCGTCG	1075	0.0	15.96195	8
CCGTCGT	1090	0.0	15.7422905	9
CGTTCGG	155	2.6728048E-7	15.6120825	14
TAGGACG	240	3.6379788E-12	15.582514	4
GTCGTAG	1110	0.0	15.260458	11
ATACGAA	695	0.0	15.195133	40
TAGTACT	325	0.0	14.891524	4
TACGAAT	720	0.0	14.667523	41
>>END_MODULE