FastQCFastQC Report
Thu 2 Feb 2017
SRR4061977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257925
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGTTCCGACCATAAACGATGC37970.11654657489045943No Hit
GTATCAACGCAGAGTACTTTTTTTT37040.11369199720681109No Hit
ATCAGATACCGTCGTAGTTCCGACC37010.11359991405572566No Hit
GGGTAGGCACACGCTGAGCCAGTCA36500.11203450048727333No Hit
GAATAGGACCGCGGTTCTATTTTGT36460.11191172295249277No Hit
GATTAAGAGGGACGGCCGGGGGCAT36320.11148200158076077No Hit
GCGCAAGACGGACCAGAGCGAAAGC34760.1066936777243184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.015.3986781
CGTTATT3250.012.8588862
GTATCAA33450.012.5191941
GCGTTAT3300.012.3733821
TCGCGTA2850.012.334589
CGACCAT7600.011.87601910
CCGACCA7750.011.7689319
CCGTCGT7250.011.6632489
CGACGCT907.4641703E-611.61157215
AACCGCG1701.0913936E-1111.1774297
CGCGTAA3150.011.15968710
CGTCGTA7500.011.14762210
GGTCGCG3250.011.1086457
GATAACG2500.011.02162311
CGAACGA2500.011.020267516
ACCGTCG7750.010.9104458
GGGTCGC3950.010.8235466
TAACGAA2650.010.75530913
ACGAACG2650.010.75514315
GTCCTAG3200.010.6828331
Produced by FastQC (version 0.11.5)