##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061977_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3257925
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28643262199099	32.0	32.0	32.0	32.0	32.0
2	30.978705464367657	32.0	32.0	32.0	32.0	32.0
3	30.991973111719883	32.0	32.0	32.0	32.0	32.0
4	30.98564423674578	32.0	32.0	32.0	32.0	32.0
5	30.958640238802307	32.0	32.0	32.0	32.0	32.0
6	34.47070451284176	36.0	36.0	36.0	32.0	36.0
7	34.450873792367844	36.0	36.0	36.0	32.0	36.0
8	34.433894580139196	36.0	36.0	36.0	32.0	36.0
9	34.53610442229333	36.0	36.0	36.0	32.0	36.0
10	34.341091338812284	36.0	36.0	36.0	32.0	36.0
11	34.46737018194096	36.0	36.0	36.0	32.0	36.0
12	34.38780604218943	36.0	36.0	36.0	32.0	36.0
13	34.432459617701454	36.0	36.0	36.0	32.0	36.0
14	34.37988996063445	36.0	36.0	36.0	32.0	36.0
15	34.332922642479495	36.0	36.0	36.0	32.0	36.0
16	34.330310550427036	36.0	36.0	36.0	32.0	36.0
17	34.278473875242675	36.0	36.0	36.0	32.0	36.0
18	34.27210939478349	36.0	36.0	36.0	32.0	36.0
19	34.273383518650675	36.0	36.0	36.0	32.0	36.0
20	34.24940905637791	36.0	36.0	36.0	32.0	36.0
21	34.221217185785434	36.0	36.0	36.0	32.0	36.0
22	34.196496543045036	36.0	36.0	36.0	32.0	36.0
23	34.17984668155344	36.0	36.0	36.0	32.0	36.0
24	34.15289916127597	36.0	36.0	36.0	32.0	36.0
25	33.7350218313804	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	57.0
5	224.0
6	688.0
7	133.0
8	581.0
9	456.0
10	253.0
11	77.0
12	120.0
13	136.0
14	479.0
15	723.0
16	1337.0
17	1876.0
18	2495.0
19	3253.0
20	4460.0
21	5689.0
22	7776.0
23	10493.0
24	14576.0
25	20391.0
26	28188.0
27	37299.0
28	51687.0
29	71504.0
30	99656.0
31	144568.0
32	219914.0
33	335968.0
34	784686.0
35	1408181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86534863769398	18.078605971033443	12.397851987249435	25.658193404023148
2	16.73492089119493	19.46448621481572	37.13288670862803	26.66770618536133
3	18.775480386433216	23.0884146771937	28.200760122017325	29.93534481435576
4	12.99821678153164	15.989781412226758	35.02251509012943	35.989486716112175
5	15.389157316066656	35.90203608905833	33.00729052035843	15.701516074516586
6	34.67883985344754	34.90728674323129	16.57827838025688	13.83559502306429
7	30.814159819550145	29.913539054604758	20.340737058127846	18.931564067717243
8	28.302417278248182	32.69388189050898	19.034760281759734	19.9689405494831
9	26.79239102142741	14.820674084379013	18.99196184017531	39.394973054018266
10	17.066582248524348	26.35607142143657	30.205999732607587	26.371346597431494
11	36.57757077770411	21.49148245514546	22.009914979770773	19.921031787379658
12	24.429294265788513	23.783987629881175	28.21916452522354	23.567553579106768
13	29.062053692030098	19.649951640845924	25.025916019496098	26.262078647627877
14	23.706219439343318	19.754445354042673	24.67457279612954	31.86476241048447
15	25.180050693571282	27.33571075343605	21.758065591838378	25.72617296115429
16	25.964350610817615	25.759684531515965	23.426737072617428	24.84922778504899
17	24.247422363588868	26.135988567740746	24.811628943284315	24.80496012538607
18	24.903980267211264	25.064200088560323	25.722164571630152	24.309655072598265
19	25.535090586879093	25.160348976396534	24.96780116358238	24.336759273141993
20	25.880013677751318	24.309468647626165	24.51156264622091	25.29895502840161
21	26.743817019222764	24.34580267611445	24.11587545465466	24.794504850008124
22	26.2191938828528	24.203178957465905	24.68920038751437	24.88842677216693
23	24.422761100724433	24.543274562412947	25.234099626509643	25.799864710352978
24	24.79007241933165	24.88764742243463	25.07785108874778	25.244429069485935
25	24.79805689767837	24.66106769245232	24.98392473612143	25.556950673747874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	136.0
1	136.0
2	501.0
3	866.0
4	866.0
5	866.0
6	1949.0
7	3032.0
8	3032.0
9	3032.0
10	3568.5
11	4105.0
12	4105.0
13	4105.0
14	6069.0
15	8033.0
16	8033.0
17	8033.0
18	13603.0
19	19173.0
20	19173.0
21	19173.0
22	31520.0
23	43867.0
24	43867.0
25	43867.0
26	66010.0
27	88153.0
28	88153.0
29	88153.0
30	110643.0
31	133133.0
32	133133.0
33	133133.0
34	167256.0
35	201379.0
36	201379.0
37	201379.0
38	235437.0
39	269495.0
40	269495.0
41	269495.0
42	308946.5
43	348398.0
44	348398.0
45	348398.0
46	382446.0
47	416494.0
48	416494.0
49	416494.0
50	427978.0
51	439462.0
52	439462.0
53	439462.0
54	410397.5
55	381333.0
56	381333.0
57	381333.0
58	349788.0
59	318243.0
60	318243.0
61	318243.0
62	281126.0
63	244009.0
64	244009.0
65	244009.0
66	203038.5
67	162068.0
68	162068.0
69	162068.0
70	124102.5
71	86137.0
72	86137.0
73	86137.0
74	65809.0
75	45481.0
76	45481.0
77	45481.0
78	36186.5
79	26892.0
80	26892.0
81	26892.0
82	18660.5
83	10429.0
84	10429.0
85	10429.0
86	7572.0
87	4715.0
88	4715.0
89	4715.0
90	3045.0
91	1375.0
92	1375.0
93	1375.0
94	838.5
95	302.0
96	302.0
97	302.0
98	758.5
99	1215.0
100	1215.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.208315108543013E-4
2	2.1486068586600365E-4
3	8.287483597688713E-4
4	0.010190535386787603
5	0.033548961378791715
6	0.06218682136636049
7	0.10411534949392635
8	0.10807492499059984
9	0.117313934482838
10	0.1313719622152137
11	0.13701972881512003
12	0.13131057344782338
13	0.12725891480006446
14	0.11482768940353139
15	0.13069668577392052
16	0.11798921092413116
17	0.12201017518819494
18	0.11191172295249277
19	0.09665661425600651
20	0.09220592862021072
21	0.09153065217891757
22	0.10239646400699831
23	0.08247580898885026
24	0.09186829039956414
25	0.08975037792459925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3257925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.807627190940096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.56532755596228	31.618648897341302
2	15.814068119993363	14.171817373855871
3	5.821203562141337	7.825029570450045
4	2.705237955987035	4.848611751785915
5	1.3923187174920937	3.1193249012176785
6	0.8538326450691032	2.295492890622639
7	0.5536392472303002	1.7365082691717608
8	0.3819380804878327	1.369099129641766
9	0.27563790819381806	1.1115612567035256
>10	1.4323132081250274	11.519391942802779
>50	0.1050970401750502	3.272920380944503
>100	0.07979547138886682	7.407740267349648
>500	0.012235462527472976	3.8230334493403557
>1k	0.00735502522648477	5.880819918772142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGTAGTTCCGACCATAAACGATGC	3797	0.11654657489045943	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3704	0.11369199720681109	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3701	0.11359991405572566	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3650	0.11203450048727333	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3646	0.11191172295249277	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3632	0.11148200158076077	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3476	0.1066936777243184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.069438369514338E-5	0.0
8	3.069438369514338E-5	0.0	0.0	3.069438369514338E-5	0.0
9	6.138876739028676E-5	0.0	0.0	3.069438369514338E-5	0.0
10	6.138876739028676E-5	0.0	0.0	3.069438369514338E-5	0.0
11	6.138876739028676E-5	0.0	0.0	6.138876739028676E-5	0.0
12	6.138876739028676E-5	0.0	0.0	6.138876739028676E-5	1.2277753478057352E-4
13	6.138876739028676E-5	0.0	0.0	6.138876739028676E-5	2.1486068586600368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1295	0.0	15.398678	1
CGTTATT	325	0.0	12.858886	2
GTATCAA	3345	0.0	12.519194	1
GCGTTAT	330	0.0	12.373382	1
TCGCGTA	285	0.0	12.33458	9
CGACCAT	760	0.0	11.876019	10
CCGACCA	775	0.0	11.768931	9
CCGTCGT	725	0.0	11.663248	9
CGACGCT	90	7.4641703E-6	11.611572	15
AACCGCG	170	1.0913936E-11	11.177429	7
CGCGTAA	315	0.0	11.159687	10
CGTCGTA	750	0.0	11.147622	10
GGTCGCG	325	0.0	11.108645	7
GATAACG	250	0.0	11.021623	11
CGAACGA	250	0.0	11.0202675	16
ACCGTCG	775	0.0	10.910445	8
GGGTCGC	395	0.0	10.823546	6
TAACGAA	265	0.0	10.755309	13
ACGAACG	265	0.0	10.755143	15
GTCCTAG	320	0.0	10.682833	1
>>END_MODULE