FastQCFastQC Report
Thu 2 Feb 2017
SRR4061977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257925
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT39390.12090517737516977No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA38550.11832684914477773No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG38000.11663865804154486No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC37730.11580990968177599No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT35580.10921061718732014No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT34270.10518965292325637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36900.026.720431
GGTATCA17000.026.410281
TAACACG708.129574E-418.8563794
ATACCGT12300.017.7066026
TAGGACG5650.017.5214144
CCGTCGT12550.017.1785879
TACCGTC12550.017.1785877
ACCGTCG12750.017.0816618
CGTCGTA12600.016.93634210
TCAACGC56900.016.8569644
CAACGCA58150.016.5324345
ATCAACG58650.016.3539853
CGCAATA8100.016.29638936
AACGCAG60300.015.9794536
TATCAAC61150.015.7585452
ATACGAA8550.015.69623640
TATACCG1704.499998E-815.5287845
CGTCTTA3850.015.4284215
GTCGTAG13800.015.30419811
TACGAAT8900.015.07943141
Produced by FastQC (version 0.11.5)