##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3257925
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161128325544635	32.0	32.0	32.0	32.0	32.0
2	31.183175180521346	32.0	32.0	32.0	32.0	32.0
3	31.42051152190428	32.0	32.0	32.0	32.0	32.0
4	31.546091760860058	32.0	32.0	32.0	32.0	32.0
5	31.477419523162748	32.0	32.0	32.0	32.0	32.0
6	35.10642418103547	36.0	36.0	36.0	36.0	36.0
7	35.11176070658471	36.0	36.0	36.0	36.0	36.0
8	35.04357988597037	36.0	36.0	36.0	36.0	36.0
9	35.18023864883323	36.0	36.0	36.0	36.0	36.0
10	35.00166455642779	36.0	36.0	36.0	36.0	36.0
11	35.18329580944926	36.0	36.0	36.0	36.0	36.0
12	35.07844103225212	36.0	36.0	36.0	36.0	36.0
13	35.13994490358127	36.0	36.0	36.0	36.0	36.0
14	35.08813708111758	36.0	36.0	36.0	36.0	36.0
15	35.04699156671808	36.0	36.0	36.0	36.0	36.0
16	35.06652332389481	36.0	36.0	36.0	36.0	36.0
17	35.0396577576218	36.0	36.0	36.0	36.0	36.0
18	35.039393478978184	36.0	36.0	36.0	36.0	36.0
19	35.03679826883676	36.0	36.0	36.0	36.0	36.0
20	35.03422976280915	36.0	36.0	36.0	36.0	36.0
21	35.02824313021325	36.0	36.0	36.0	36.0	36.0
22	35.00860394269358	36.0	36.0	36.0	36.0	36.0
23	34.97183329880215	36.0	36.0	36.0	32.0	36.0
24	34.951827927285	36.0	36.0	36.0	32.0	36.0
25	34.93403132361856	36.0	36.0	36.0	32.0	36.0
26	34.8867417144348	36.0	36.0	36.0	32.0	36.0
27	34.87233591933516	36.0	36.0	36.0	32.0	36.0
28	34.85305432138554	36.0	36.0	36.0	32.0	36.0
29	34.82204132998765	36.0	36.0	36.0	32.0	36.0
30	34.8007609137718	36.0	36.0	36.0	32.0	36.0
31	34.80800141194165	36.0	36.0	36.0	32.0	36.0
32	34.779103570524185	36.0	36.0	36.0	32.0	36.0
33	34.75694253244013	36.0	36.0	36.0	32.0	36.0
34	34.74563993953206	36.0	36.0	36.0	32.0	36.0
35	34.73784479384884	36.0	36.0	36.0	32.0	36.0
36	34.701970732905146	36.0	36.0	36.0	32.0	36.0
37	34.69289471059033	36.0	36.0	36.0	32.0	36.0
38	34.66848715056363	36.0	36.0	36.0	32.0	36.0
39	34.6480766131817	36.0	36.0	36.0	32.0	36.0
40	34.63844962667956	36.0	36.0	36.0	32.0	36.0
41	34.60802566050477	36.0	36.0	36.0	32.0	36.0
42	34.5761298986318	36.0	36.0	36.0	32.0	36.0
43	34.58258063030917	36.0	36.0	36.0	32.0	36.0
44	34.524015132331165	36.0	36.0	36.0	32.0	36.0
45	34.51074809886661	36.0	36.0	36.0	32.0	36.0
46	34.48939125363537	36.0	36.0	36.0	32.0	36.0
47	34.47648242362854	36.0	36.0	36.0	32.0	36.0
48	34.44393164360751	36.0	36.0	36.0	32.0	36.0
49	34.429695588449704	36.0	36.0	36.0	32.0	36.0
50	33.954148729636195	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	26.0
21	74.0
22	214.0
23	574.0
24	1434.0
25	3621.0
26	7488.0
27	14901.0
28	26599.0
29	44113.0
30	68702.0
31	106098.0
32	169460.0
33	299392.0
34	671169.0
35	1844055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58226313703251	18.61228134114144	12.85727751394937	25.948178007876677
2	16.259201051022476	20.01347543419138	36.678719864447565	27.048603650338578
3	18.269743720076786	23.63036929674805	28.415597426826057	29.684289556349107
4	12.89909988719814	16.475179753984513	35.15608861468573	35.46963174413162
5	15.238876044990612	36.269661293510836	32.61509476586931	15.876367895629242
6	34.958203419234714	35.03610583678799	16.357819493529924	13.647871250447372
7	30.918544779269013	29.92610327125394	20.305102173929725	18.85024977554732
8	28.44865980647191	32.81122186667895	18.792114612828716	19.948003714020427
9	27.03395566196275	14.49428700783474	18.81366207018271	39.6580952600198
10	17.10913186661064	26.37313796129495	30.187656483997007	26.33007368809741
11	36.99191970349226	21.26206097439321	21.949645863548117	19.796373458566418
12	24.60888448936056	23.615706316136805	28.117805044621964	23.657604149880676
13	29.319981276425942	19.44016513578428	25.03369475970134	26.206158828088434
14	23.89210924131157	19.55259866325959	24.584758703776178	31.970533391652662
15	25.35070635450478	27.26493089926871	21.601694329979974	25.782668416246533
16	26.203382547039507	25.610638755026244	23.336474416034868	24.84950428189938
17	24.427388598571177	26.050814552207306	24.68528281037777	24.83651403884374
18	25.204102492324864	24.9080855833872	25.619336497313316	24.268475426974618
19	25.75422262766105	24.979680311756812	24.939532045560302	24.326565015021835
20	26.13850317902415	24.186121320426114	24.3763655193286	25.299009981221136
21	26.987729920117864	24.191348787955526	24.044015746218836	24.776905545707777
22	26.343239945670945	24.20970402940522	24.607687408396448	24.83936861652739
23	24.685873580844735	24.365915226997316	25.205591045094977	25.74262014706298
24	25.12244113678415	24.74689154827723	24.964578322424437	25.16608899251418
25	25.152920029761393	24.425675094968803	24.96077242110898	25.460632454160827
26	24.94469615652484	25.29841975425357	25.138286466389044	24.618597622832546
27	25.546308662207164	24.68545027780071	24.62706930913705	25.141171750855072
28	24.98354754269044	24.48233079752673	25.322633915635727	25.2114877441471
29	24.920623909576328	24.700359862832222	25.208109007375285	25.170907220216165
30	24.911176982107705	24.76669665546614	25.374975098860208	24.947151263565942
31	25.617542769724906	24.452993205464114	24.53313656108575	25.396327463725232
32	25.478229400936737	24.53891352811916	24.238720428447376	25.744136642496724
33	24.902936446815556	24.52036070900517	24.924115623571748	25.652587220607526
34	25.63436647455904	24.518769168813247	25.09220674938672	24.754657607240993
35	25.953852165230522	24.291841330713666	25.12909306272313	24.625213441332676
36	24.777211668942662	24.903703690061704	24.791791554900332	25.5272930860953
37	25.95890932352078	24.62776269634006	24.511060607734876	24.902267372404285
38	25.00155773997253	24.382636187143657	24.95416561154723	25.66164046133659
39	25.790691449850637	24.507536738299546	24.248751039010312	25.4530207728395
40	25.861798537412618	24.612782676089843	24.805512711311646	24.719906075185893
41	24.90642716604919	25.1372821059029	25.09409464407945	24.862196083968456
42	26.103377463725185	24.9597424004764	24.618220439134745	24.31865969666367
43	25.603247853907966	24.160671278802575	24.786741408725323	25.44933945856414
44	25.047417186830927	24.772322298739788	24.60196114844902	25.57829936598026
45	25.34525816396816	25.00365271350571	24.45774086729538	25.193348255230745
46	25.069301776353935	24.806705520476218	24.657803774449	25.466188928720847
47	25.406760292055225	24.539968820185663	24.95886124614281	25.094409641616306
48	25.82357287324187	25.2072035403174	24.039539724358896	24.929683862081838
49	25.219734311335447	25.140263454026307	24.335885773151066	25.304116461487176
50	25.300258820524913	25.255566851327988	24.289317882681125	25.154856445465974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	17.0
2	26.0
3	73.5
4	121.0
5	166.5
6	212.0
7	197.5
8	183.0
9	252.5
10	322.0
11	490.0
12	658.0
13	1350.5
14	2043.0
15	2634.5
16	3226.0
17	3593.5
18	3961.0
19	4268.0
20	4575.0
21	5888.5
22	7202.0
23	8848.0
24	10494.0
25	13996.0
26	17498.0
27	22722.5
28	27947.0
29	32675.5
30	37404.0
31	43764.5
32	50125.0
33	58683.0
34	67241.0
35	78236.0
36	89231.0
37	105854.5
38	122478.0
39	132676.5
40	142875.0
41	156217.5
42	169560.0
43	173668.0
44	177776.0
45	194708.5
46	211641.0
47	223836.0
48	236031.0
49	245364.5
50	254698.0
51	245939.5
52	237181.0
53	236287.0
54	235393.0
55	236057.5
56	236722.0
57	229639.5
58	222557.0
59	204722.0
60	186887.0
61	164848.5
62	142810.0
63	124628.5
64	106447.0
65	89604.5
66	72762.0
67	65444.5
68	58127.0
69	51910.0
70	45693.0
71	35739.0
72	25785.0
73	22636.5
74	19488.0
75	14920.5
76	10353.0
77	9061.0
78	7769.0
79	6573.5
80	5378.0
81	4201.5
82	3025.0
83	2549.5
84	2074.0
85	1588.5
86	1103.0
87	765.0
88	427.0
89	283.5
90	140.0
91	114.0
92	88.0
93	70.5
94	53.0
95	51.0
96	49.0
97	51.5
98	54.0
99	39.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029712163416898797
2	0.00445068563579579
3	9.208315108543014E-5
4	0.0
5	3.069438369514338E-5
6	9.208315108543014E-5
7	0.0
8	0.0
9	0.0
10	1.2277753478057352E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.673595923785846E-4
17	0.0
18	9.208315108543014E-5
19	3.069438369514338E-5
20	2.1486068586600365E-4
21	0.0
22	0.0
23	3.069438369514338E-5
24	0.0010129146619397315
25	3.9902698803686394E-4
26	4.297213717320073E-4
27	4.297213717320073E-4
28	0.0016268023358425993
29	5.218045228174374E-4
30	0.001289164115196022
31	3.9902698803686394E-4
32	6.752764412931544E-4
33	2.4555506956114705E-4
34	8.90137127159158E-4
35	1.2277753478057352E-4
36	3.6833260434172057E-4
37	0.0017495798706231728
38	0.0
39	5.218045228174374E-4
40	0.0
41	0.0010743034293300183
42	0.0047576294727472235
43	0.004389296868405504
44	0.004297213717320073
45	0.0022406900097454666
46	0.0022099956260503233
47	0.0014733304173668823
48	0.004235824949929787
49	0.0038981867292832094
50	0.0021179124749648934
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3257925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.34159863201996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6124092460834	32.722919277219326
2	15.89180049085496	14.729028797746766
3	5.870882758610702	8.161982772455499
4	2.6719128946129116	4.952828597674808
5	1.4121038096495282	3.271957398676237
6	0.8378320236463862	2.329588521652433
7	0.5192647573514266	1.6844491278253135
8	0.3665171346130623	1.358799195519725
9	0.28246205431676774	1.1780768834931123
>10	1.3528188978908329	11.254327850858425
>50	0.09315125131685735	3.0046716822676665
>100	0.07234230847478501	6.932653792460468
>500	0.010380524686941076	3.3679058181123165
>1k	0.0061218478913531525	5.050810284037978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3939	0.12090517737516977	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3855	0.11832684914477773	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3800	0.11663865804154486	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3773	0.11580990968177599	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3558	0.10921061718732014	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3427	0.10518965292325637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.138876739028676E-5
2	0.0	0.0	0.0	0.0	6.138876739028676E-5
3	0.0	0.0	0.0	0.0	6.138876739028676E-5
4	0.0	0.0	0.0	0.0	6.138876739028676E-5
5	3.069438369514338E-5	0.0	0.0	0.0	6.138876739028676E-5
6	3.069438369514338E-5	0.0	0.0	0.0	6.138876739028676E-5
7	3.069438369514338E-5	0.0	0.0	0.0	6.138876739028676E-5
8	3.069438369514338E-5	0.0	0.0	0.0	6.138876739028676E-5
9	3.069438369514338E-5	0.0	0.0	3.069438369514338E-5	6.138876739028676E-5
10	3.069438369514338E-5	0.0	0.0	6.138876739028676E-5	6.138876739028676E-5
11	3.069438369514338E-5	0.0	0.0	1.2277753478057352E-4	6.138876739028676E-5
12	3.069438369514338E-5	0.0	0.0	1.2277753478057352E-4	1.5347191847571692E-4
13	3.069438369514338E-5	0.0	0.0	1.2277753478057352E-4	2.1486068586600368E-4
14	3.069438369514338E-5	0.0	0.0	1.5347191847571692E-4	2.1486068586600368E-4
15	3.069438369514338E-5	0.0	0.0	1.8416630217086029E-4	3.376382206465772E-4
16	3.069438369514338E-5	0.0	0.0	2.1486068586600368E-4	3.376382206465772E-4
17	3.069438369514338E-5	0.0	0.0	2.4555506956114705E-4	3.376382206465772E-4
18	3.069438369514338E-5	0.0	0.0	2.4555506956114705E-4	3.376382206465772E-4
19	3.069438369514338E-5	0.0	0.0	2.762494532562904E-4	3.376382206465772E-4
20	3.069438369514338E-5	0.0	0.0	3.9902698803686394E-4	3.9902698803686394E-4
21	3.069438369514338E-5	0.0	0.0	4.911101391222941E-4	4.911101391222941E-4
22	3.069438369514338E-5	0.0	0.0	6.138876739028677E-4	5.218045228174375E-4
23	3.069438369514338E-5	0.0	0.0	8.287483597688713E-4	5.218045228174375E-4
24	3.069438369514338E-5	0.0	0.0	0.0010129146619397315	5.218045228174375E-4
25	3.069438369514338E-5	0.0	0.0	0.0011970809641105918	5.218045228174375E-4
26	3.069438369514338E-5	0.0	0.0	0.0015040248010620256	5.524989065125808E-4
27	3.069438369514338E-5	0.0	0.0	0.001780274254318316	5.524989065125808E-4
28	3.069438369514338E-5	0.0	0.0	0.0022099956260503233	5.524989065125808E-4
29	3.069438369514338E-5	0.0	0.0	0.003222910287990055	5.524989065125808E-4
30	3.069438369514338E-5	0.0	0.0	0.004604157554271507	5.524989065125808E-4
31	3.069438369514338E-5	0.0	0.0	0.0062923486575043935	5.524989065125808E-4
32	3.069438369514338E-5	0.0	0.0	0.009239009492238158	5.524989065125808E-4
33	3.069438369514338E-5	0.0	0.0	0.011817337722630201	5.524989065125808E-4
34	3.069438369514338E-5	0.0	0.0	0.014794692941059109	5.524989065125808E-4
35	3.069438369514338E-5	0.0	0.0	0.018048297612744308	5.524989065125808E-4
36	3.069438369514338E-5	0.0	0.0	0.02222273379528381	5.524989065125808E-4
37	3.069438369514338E-5	0.0	0.0	0.02857647122017849	5.524989065125808E-4
38	3.069438369514338E-5	0.0	0.0	0.03787686947980693	5.524989065125808E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3690	0.0	26.72043	1
GGTATCA	1700	0.0	26.41028	1
TAACACG	70	8.129574E-4	18.856379	4
ATACCGT	1230	0.0	17.706602	6
TAGGACG	565	0.0	17.521414	4
CCGTCGT	1255	0.0	17.178587	9
TACCGTC	1255	0.0	17.178587	7
ACCGTCG	1275	0.0	17.081661	8
CGTCGTA	1260	0.0	16.936342	10
TCAACGC	5690	0.0	16.856964	4
CAACGCA	5815	0.0	16.532434	5
ATCAACG	5865	0.0	16.353985	3
CGCAATA	810	0.0	16.296389	36
AACGCAG	6030	0.0	15.979453	6
TATCAAC	6115	0.0	15.758545	2
ATACGAA	855	0.0	15.696236	40
TATACCG	170	4.499998E-8	15.528784	5
CGTCTTA	385	0.0	15.42842	15
GTCGTAG	1380	0.0	15.304198	11
TACGAAT	890	0.0	15.079431	41
>>END_MODULE