FastQCFastQC Report
Thu 2 Feb 2017
SRR4061976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4413286
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68590.15541707471484967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.013.1669811
GTATCAA37900.012.9548411
CGCGCTT803.7724478E-410.68786312
GTATTAC4050.010.3175931
GTATTAA4400.010.1443891
ATTACAC5000.09.878883
TTACACA7800.09.74325754
ACACCGT2500.09.5005396
AATATCG1201.701631E-59.5002165
CTATACT4250.09.3879164
GTAGGAC5550.09.2421943
ATCCTAG4850.09.2031571
TACGAGT1252.757706E-59.1196894
GTCTAGA5450.09.0612371
ATAGGAC3600.08.9712053
GTCTTAG4800.08.903321
CTTACAC5250.08.8656613
GGACCGA3450.08.8120946
TAGGACT5300.08.782724
TATCAAC55100.08.7745682
Produced by FastQC (version 0.11.5)