##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061976_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4413286
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24983991520151	32.0	32.0	32.0	32.0	32.0
2	30.759021509143075	32.0	32.0	32.0	32.0	32.0
3	30.796264280175816	32.0	32.0	32.0	32.0	32.0
4	30.80433559030618	32.0	32.0	32.0	32.0	32.0
5	30.70613483921051	32.0	32.0	32.0	32.0	32.0
6	34.36069495609394	36.0	36.0	36.0	32.0	36.0
7	34.30631416137545	36.0	36.0	36.0	32.0	36.0
8	34.257084856952396	36.0	36.0	36.0	32.0	36.0
9	34.400179594071176	36.0	36.0	36.0	32.0	36.0
10	34.06295694410016	36.0	36.0	36.0	32.0	36.0
11	34.386147872582924	36.0	36.0	36.0	32.0	36.0
12	34.20822987678569	36.0	36.0	36.0	32.0	36.0
13	34.279980948436155	36.0	36.0	36.0	32.0	36.0
14	34.198429242972246	36.0	36.0	36.0	32.0	36.0
15	34.15142231887985	36.0	36.0	36.0	32.0	36.0
16	34.14761608470423	36.0	36.0	36.0	32.0	36.0
17	34.09099274327564	36.0	36.0	36.0	32.0	36.0
18	34.087006824393434	36.0	36.0	36.0	32.0	36.0
19	34.09389149037701	36.0	36.0	36.0	32.0	36.0
20	34.07496658952083	36.0	36.0	36.0	32.0	36.0
21	34.06580788102108	36.0	36.0	36.0	32.0	36.0
22	34.03823522880683	36.0	36.0	36.0	32.0	36.0
23	33.99352591243803	36.0	36.0	36.0	32.0	36.0
24	33.9774211324623	36.0	36.0	36.0	32.0	36.0
25	33.60979936491766	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	68.0
5	348.0
6	952.0
7	181.0
8	734.0
9	606.0
10	339.0
11	110.0
12	180.0
13	185.0
14	620.0
15	905.0
16	1441.0
17	2025.0
18	2845.0
19	3973.0
20	5938.0
21	8529.0
22	12911.0
23	19260.0
24	27210.0
25	38356.0
26	52203.0
27	67311.0
28	89256.0
29	118121.0
30	155270.0
31	209824.0
32	296453.0
33	420848.0
34	914467.0
35	1961810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07127304911427	16.871689931136746	12.213931763042972	25.843105256706018
2	16.596673310257504	19.148791056802246	37.49253389092366	26.76200174201659
3	19.518617580521195	22.596441441768057	27.666108951955565	30.218832025755184
4	13.411038746198575	15.483183089271712	34.557675137440455	36.548103027089255
5	15.405496623458696	35.729820482197255	33.429220925965424	15.435461968378627
6	35.295522055067444	33.9393440582358	16.61493763834936	14.150196248347402
7	31.09436620932352	29.644169702842643	19.932671068157784	19.328793019676056
8	28.109865048203876	33.25803120044333	18.784483390242812	19.847620361109982
9	26.896814721937833	15.19990308664059	18.219824997884558	39.68345719353702
10	16.190026298886124	27.385283559894912	31.45753822368733	24.96715191753163
11	36.34349108411514	21.691016133592374	21.949055587398274	20.016437194894213
12	24.63577003386766	23.316208536956413	28.64434733107519	23.403674098100737
13	29.780091232372108	20.045779538556406	24.43769304522187	25.73643618384961
14	23.403814647633506	19.209281138686702	25.368373151101842	32.01853106257796
15	25.14199443616416	26.7678996485676	22.18058257141243	25.90952334385581
16	25.530533464488332	26.090127622041297	23.38183436720738	24.99750454626299
17	24.323484905202356	25.928431579401106	24.923601516399298	24.824481998997232
18	24.495448431052747	25.87720631435202	25.341679577712394	24.28566567688284
19	25.648250401509827	25.133823378316496	24.884945122265158	24.332981097908522
20	25.628651741201008	24.911106282820594	24.994023856492724	24.466218119485674
21	25.666513428244386	24.713630500962637	24.923394018621302	24.696462052171672
22	25.593258245507073	24.487173554858753	24.795291314635776	25.1242768849984
23	24.933543900484345	24.741216932392796	24.93950805478314	25.38573111233972
24	24.757012703196256	24.750843901186677	25.189237073411462	25.302906322205597
25	25.107198753190108	24.638216256290917	25.086900898616637	25.167684091902338
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	462.0
1	462.0
2	875.0
3	1288.0
4	1288.0
5	1288.0
6	2534.0
7	3780.0
8	3780.0
9	3780.0
10	4804.0
11	5828.0
12	5828.0
13	5828.0
14	7984.0
15	10140.0
16	10140.0
17	10140.0
18	18284.0
19	26428.0
20	26428.0
21	26428.0
22	40599.0
23	54770.0
24	54770.0
25	54770.0
26	79706.5
27	104643.0
28	104643.0
29	104643.0
30	137581.0
31	170519.0
32	170519.0
33	170519.0
34	212397.0
35	254275.0
36	254275.0
37	254275.0
38	308419.5
39	362564.0
40	362564.0
41	362564.0
42	415429.0
43	468294.0
44	468294.0
45	468294.0
46	516023.5
47	563753.0
48	563753.0
49	563753.0
50	589129.5
51	614506.0
52	614506.0
53	614506.0
54	594314.0
55	574122.0
56	574122.0
57	574122.0
58	524153.5
59	474185.0
60	474185.0
61	474185.0
62	406682.0
63	339179.0
64	339179.0
65	339179.0
66	272716.0
67	206253.0
68	206253.0
69	206253.0
70	154174.5
71	102096.0
72	102096.0
73	102096.0
74	73749.0
75	45402.0
76	45402.0
77	45402.0
78	31948.5
79	18495.0
80	18495.0
81	18495.0
82	12693.5
83	6892.0
84	6892.0
85	6892.0
86	4705.5
87	2519.0
88	2519.0
89	2519.0
90	1686.5
91	854.0
92	854.0
93	854.0
94	608.5
95	363.0
96	363.0
97	363.0
98	1019.5
99	1676.0
100	1676.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010649661046213637
2	2.265885328981625E-4
3	8.610364250130175E-4
4	0.010559025633054372
5	0.03450943356039015
6	0.06351276577135495
7	0.1083093187253217
8	0.10928364941678377
9	0.1183925084392899
10	0.13312076307767048
11	0.1406661612231793
12	0.13225972665265745
13	0.12779593255456367
14	0.1160133288438592
15	0.1335739401434668
16	0.11941215683733163
17	0.12407988061503379
18	0.11139092277273668
19	0.09750104570607933
20	0.09262939224876883
21	0.09142847302440857
22	0.10382286577393805
23	0.08175314266965704
24	0.09068073086584463
25	0.08957044705464363
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4413286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.695420010573116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.769394599922656	20.977982240113466
2	15.541043123675452	11.09488123404049
3	8.035095039401039	8.604482767688781
4	4.762155796115352	6.799486051924908
5	3.058499742744221	5.458721645974239
6	2.0602401368588685	4.412468220469076
7	1.5001165115768136	3.74831022618711
8	1.1473003563705038	3.2762694479140277
9	0.8406402161810659	2.700630503492592
>10	4.065933302791375	24.410558650695876
>50	0.15757640826078284	3.8327726397467177
>100	0.059196281792331135	3.6095535885443257
>500	0.0017872174129508055	0.40259501395676
>1k	9.574377156343866E-4	0.5150116667062365
>5k	6.38291810422743E-5	0.15627610254545127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6859	0.15541707471484967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.797655986944875E-5
2	0.0	0.0	0.0	0.0	6.797655986944875E-5
3	0.0	0.0	0.0	0.0	6.797655986944875E-5
4	0.0	6.797655986944875E-5	0.0	0.0	6.797655986944875E-5
5	0.0	6.797655986944875E-5	0.0	0.0	6.797655986944875E-5
6	0.0	6.797655986944875E-5	0.0	0.0	6.797655986944875E-5
7	0.0	6.797655986944875E-5	0.0	0.0	9.0635413159265E-5
8	0.0	6.797655986944875E-5	0.0	2.265885328981625E-5	9.0635413159265E-5
9	0.0	1.1329426644908125E-4	0.0	4.53177065796325E-5	9.0635413159265E-5
10	0.0	1.1329426644908125E-4	0.0	9.0635413159265E-5	9.0635413159265E-5
11	0.0	1.1329426644908125E-4	0.0	9.0635413159265E-5	1.1329426644908125E-4
12	0.0	1.1329426644908125E-4	0.0	9.0635413159265E-5	4.7583591908614126E-4
13	0.0	1.1329426644908125E-4	0.0	1.1329426644908125E-4	6.34447892114855E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1255	0.0	13.166981	1
GTATCAA	3790	0.0	12.954841	1
CGCGCTT	80	3.7724478E-4	10.687863	12
GTATTAC	405	0.0	10.317593	1
GTATTAA	440	0.0	10.144389	1
ATTACAC	500	0.0	9.87888	3
TTACACA	780	0.0	9.7432575	4
ACACCGT	250	0.0	9.500539	6
AATATCG	120	1.701631E-5	9.500216	5
CTATACT	425	0.0	9.387916	4
GTAGGAC	555	0.0	9.242194	3
ATCCTAG	485	0.0	9.203157	1
TACGAGT	125	2.757706E-5	9.119689	4
GTCTAGA	545	0.0	9.061237	1
ATAGGAC	360	0.0	8.971205	3
GTCTTAG	480	0.0	8.90332	1
CTTACAC	525	0.0	8.865661	3
GGACCGA	345	0.0	8.812094	6
TAGGACT	530	0.0	8.78272	4
TATCAAC	5510	0.0	8.774568	2
>>END_MODULE