FastQCFastQC Report
Thu 2 Feb 2017
SRR4061976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4413286
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40050.026.758131
GGTATCA16900.023.6981241
TATCAAC59450.017.9101722
ATCAACG59850.017.7169573
TCAACGC60250.017.6358474
CAACGCA62100.017.1813145
AACGCAG64900.016.4061586
GTATAGA7950.015.5006591
GTATAGG5900.014.1729691
GGACCGA8750.013.8280486
ACGCAGA79000.013.5336657
GTCCTAA5650.013.24218751
AGAGTAC82050.013.05754511
CGCAGAG82000.013.0117038
TATACTG7450.012.9927965
GTACATG99550.012.7987211
CAGAGTA85500.012.63358310
AGGACCG13250.012.6183915
CTAGTAC2452.2202585E-812.5709533
TTACACG2808.367351E-1012.5709534
Produced by FastQC (version 0.11.5)