##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4413286
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30102989019973	32.0	32.0	32.0	32.0	32.0
2	31.4563486254913	32.0	32.0	32.0	32.0	32.0
3	31.57179752229971	32.0	32.0	32.0	32.0	32.0
4	31.64836269392013	32.0	32.0	32.0	32.0	32.0
5	31.62108347385599	32.0	32.0	32.0	32.0	32.0
6	35.26327525567117	36.0	36.0	36.0	36.0	36.0
7	35.275004611076646	36.0	36.0	36.0	36.0	36.0
8	35.23021530895573	36.0	36.0	36.0	36.0	36.0
9	35.31310751218027	36.0	36.0	36.0	36.0	36.0
10	35.19465631731096	36.0	36.0	36.0	36.0	36.0
11	35.313952687407976	36.0	36.0	36.0	36.0	36.0
12	35.24980388762478	36.0	36.0	36.0	36.0	36.0
13	35.268333391491055	36.0	36.0	36.0	36.0	36.0
14	35.235550789139886	36.0	36.0	36.0	36.0	36.0
15	35.21194298307429	36.0	36.0	36.0	36.0	36.0
16	35.233335206465206	36.0	36.0	36.0	36.0	36.0
17	35.2144556233156	36.0	36.0	36.0	36.0	36.0
18	35.20680304879403	36.0	36.0	36.0	36.0	36.0
19	35.2050492535494	36.0	36.0	36.0	36.0	36.0
20	35.201194756016264	36.0	36.0	36.0	36.0	36.0
21	35.19234556745246	36.0	36.0	36.0	36.0	36.0
22	35.17471403394205	36.0	36.0	36.0	36.0	36.0
23	35.1434667954898	36.0	36.0	36.0	36.0	36.0
24	35.13589216742355	36.0	36.0	36.0	36.0	36.0
25	35.1202818036266	36.0	36.0	36.0	36.0	36.0
26	35.080855398902315	36.0	36.0	36.0	36.0	36.0
27	35.06696778772098	36.0	36.0	36.0	36.0	36.0
28	35.05293244081621	36.0	36.0	36.0	36.0	36.0
29	35.031853815954825	36.0	36.0	36.0	36.0	36.0
30	35.012134269113766	36.0	36.0	36.0	36.0	36.0
31	35.00357556704913	36.0	36.0	36.0	36.0	36.0
32	34.98319460828054	36.0	36.0	36.0	36.0	36.0
33	34.963757390751475	36.0	36.0	36.0	36.0	36.0
34	34.95481054252999	36.0	36.0	36.0	36.0	36.0
35	34.94668235867786	36.0	36.0	36.0	36.0	36.0
36	34.920205488608715	36.0	36.0	36.0	36.0	36.0
37	34.909281655437695	36.0	36.0	36.0	36.0	36.0
38	34.902904774356344	36.0	36.0	36.0	36.0	36.0
39	34.879376500865796	36.0	36.0	36.0	32.0	36.0
40	34.87185036274558	36.0	36.0	36.0	32.0	36.0
41	34.845531878060925	36.0	36.0	36.0	32.0	36.0
42	34.83195038798755	36.0	36.0	36.0	32.0	36.0
43	34.81105099465568	36.0	36.0	36.0	32.0	36.0
44	34.783655081497095	36.0	36.0	36.0	32.0	36.0
45	34.76904601242702	36.0	36.0	36.0	32.0	36.0
46	34.7496180850278	36.0	36.0	36.0	32.0	36.0
47	34.71862145349293	36.0	36.0	36.0	32.0	36.0
48	34.70865019851421	36.0	36.0	36.0	32.0	36.0
49	34.681440314541135	36.0	36.0	36.0	32.0	36.0
50	34.277333488017774	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	13.0
20	37.0
21	78.0
22	262.0
23	783.0
24	2014.0
25	4685.0
26	9971.0
27	19171.0
28	33054.0
29	52387.0
30	79200.0
31	118249.0
32	178519.0
33	303648.0
34	696976.0
35	2914236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70390837070227	17.411914782504628	12.568678454276922	26.31549839251618
2	16.038820739592648	19.52627458444791	37.17489160126487	27.260013074694573
3	18.86432560694197	23.005244181641196	27.893476767350844	30.23695344406599
4	13.175534057842613	15.91868281366764	34.64384587810534	36.261937250384406
5	15.129569017183345	36.05386917001734	33.26134614012011	15.555215672679196
6	35.494878071384115	34.10962687833695	16.41971876239866	13.975776287880281
7	31.158279794239483	29.658603589252998	19.921890400939347	19.261226215568172
8	28.190899026258435	33.287736167563125	18.696001120253708	19.825363685924728
9	27.05131731775371	14.937939666724523	18.126584137080624	39.88415887844114
10	16.13964392032959	27.38936691559705	31.553546770861228	24.917442393212127
11	36.61983383809705	21.494097595306535	21.951783772907536	19.934284793688875
12	24.73975734628514	23.249665498602514	28.652398383517042	23.35817877159531
13	29.925162339354394	19.89290972758167	24.48894089347484	25.692987039589095
14	23.439201538264232	19.056322205268366	25.325460439228276	32.179015817239126
15	25.218827875646397	26.73710699918383	22.14220877595515	25.90185634921462
16	25.656266420680762	25.961641516329987	23.387966403981093	24.994125659008155
17	24.42236918250936	25.813939998450135	25.01018515455377	24.753505664486735
18	24.647025488304728	25.763021100637417	25.33986634427599	24.250087066781862
19	25.727360520029745	25.019905802615106	24.94472372739949	24.308009949955654
20	25.786994918739488	24.774141652174407	25.021984807201004	24.416878621885107
21	25.793587222935848	24.632048296019086	24.900329301338708	24.674035179706355
22	25.671755694056536	24.45447677762103	24.84411841879271	25.02964910952972
23	25.03606159769532	24.688270543328073	24.957639235380835	25.318028623595772
24	24.92410899301352	24.629495311491315	25.115193224383823	25.331202471111343
25	25.21700452634689	24.489086555281432	25.214942564156477	25.078966354215193
26	25.054619413377754	24.936249027364315	25.052965309591578	24.95616624966635
27	25.134996547904148	25.010372148848663	24.802498651224116	25.052132652023072
28	25.262223620043244	24.707707618341086	24.874728599402975	25.155340162212696
29	25.104820472847738	24.938571486837393	24.890421139928307	25.066186900386562
30	25.352378522454	24.902933751591526	24.750754720529645	24.993933005424836
31	25.221230716661992	24.73573992594147	24.88071192626957	25.16231743112697
32	25.096425536735968	24.853248739591006	24.88852886194981	25.161796861723218
33	24.963156621067885	24.815579175197744	24.96476540329678	25.256498800437587
34	25.20769817289053	24.765439567847313	25.113187431952156	24.91367482731
35	25.262560176413245	24.733406536734407	25.132883206435856	24.87115008041649
36	25.259608793922883	24.8711671037948	24.8165591312574	25.052664971024917
37	25.333962352222482	24.726854891958755	24.873686390578133	25.065496365240637
38	25.1794524940039	24.943505801234227	24.73601863464517	25.141023070116702
39	25.363320599712097	24.906559812709496	24.7012916328575	25.028827954720906
40	25.455556451839488	24.774974826002584	24.690411947203035	25.07905677495489
41	25.161717921527043	24.788069536213754	24.926743163752707	25.123469378506496
42	25.301858134098087	24.815916673219153	24.778482525708217	25.103742666974536
43	25.27594964474146	24.550590466073466	24.945367499304346	25.22809238988073
44	25.019589448662956	24.786578111935174	24.885057861951605	25.308774577450265
45	25.066771201428438	24.873487635403297	24.899092609566065	25.1606485536022
46	25.350840878341096	24.733055483989673	24.61028695161746	25.30581668605177
47	25.114451475837168	25.00838389349827	24.849860328866153	25.027304301798413
48	25.06032024566791	25.00344428149569	24.894745476661367	25.041489996175038
49	25.3089811705199	24.75669779270192	24.60977269436475	25.32454834241343
50	25.203780195417803	25.104578049859843	24.725534310505434	24.96610744421692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	96.5
2	96.0
3	260.0
4	424.0
5	486.5
6	549.0
7	459.0
8	369.0
9	478.5
10	588.0
11	1088.0
12	1588.0
13	2780.5
14	3973.0
15	5239.0
16	6505.0
17	6290.0
18	6075.0
19	6093.0
20	6111.0
21	7392.0
22	8673.0
23	11089.0
24	13505.0
25	17631.0
26	21757.0
27	27561.5
28	33366.0
29	40869.0
30	48372.0
31	57726.0
32	67080.0
33	78196.5
34	89313.0
35	103368.0
36	117423.0
37	131961.0
38	146499.0
39	162602.5
40	178706.0
41	198466.0
42	218226.0
43	236473.5
44	254721.0
45	274728.5
46	294736.0
47	305719.0
48	316702.0
49	327072.5
50	337443.0
51	345983.0
52	354523.0
53	357453.5
54	360384.0
55	351494.5
56	342605.0
57	327200.0
58	311795.0
59	289220.0
60	266645.0
61	232644.0
62	198643.0
63	171073.5
64	143504.0
65	121704.5
66	99905.0
67	82051.5
68	64198.0
69	52084.0
70	39970.0
71	31356.5
72	22743.0
73	19049.0
74	15355.0
75	12164.5
76	8974.0
77	7091.5
78	5209.0
79	4066.5
80	2924.0
81	2247.0
82	1570.0
83	1157.0
84	744.0
85	545.5
86	347.0
87	251.5
88	156.0
89	102.0
90	48.0
91	41.5
92	35.0
93	33.0
94	31.0
95	36.0
96	41.0
97	37.0
98	33.0
99	20.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032220889378118706
2	0.0041012524454567415
3	3.172239460574275E-4
4	0.0
5	2.265885328981625E-5
6	1.5861197302871374E-4
7	0.0
8	0.0
9	0.0
10	9.0635413159265E-5
11	0.0
12	2.265885328981625E-5
13	0.0
14	0.0
15	0.0
16	5.664713322454062E-4
17	0.0
18	1.5861197302871374E-4
19	0.0
20	2.4924738618797875E-4
21	6.797655986944875E-5
22	0.0
23	9.0635413159265E-5
24	0.0011102838112009962
25	3.172239460574275E-4
26	4.53177065796325E-4
27	5.664713322454062E-4
28	0.001540802023707505
29	5.891301855352226E-4
30	0.0014275077572584237
31	4.53177065796325E-4
32	8.15718718433385E-4
33	2.265885328981625E-4
34	7.024244519843037E-4
35	2.71906239477795E-4
36	2.4924738618797875E-4
37	0.00145016661054824
38	2.265885328981625E-5
39	7.930598651435687E-4
40	4.53177065796325E-5
41	0.0010423072513315475
42	0.004599747217832699
43	0.003670734232950232
44	0.004441135244803985
45	0.001835367116475116
46	0.002333861888851074
47	0.0014048489039686074
48	0.003942640472428028
49	0.003466804553341886
50	0.0016994139967362188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4413286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.549255243383335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.824621691194785	20.20072980608981
2	16.413957435457522	11.670079248542184
3	8.497470241498384	9.06236215614241
4	4.918877476060601	6.9944972362963
5	3.184937812813731	5.661108362100919
6	2.1401527844849233	4.564850255729526
7	1.563679523964092	3.891134976137616
8	1.19316887431391	3.393301188915645
9	0.8916171926541323	2.8526694444945577
>10	4.1699705507237255	24.84706357363054
>50	0.14891593317684554	3.5541041380504588
>100	0.051476915239811474	2.9640839564028085
>500	7.690458376349271E-4	0.16107229832511377
>1k	3.845225799959713E-4	0.18294335914208815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.359531197388975E-4
2	0.0	0.0	0.0	0.0	1.359531197388975E-4
3	0.0	0.0	0.0	2.265885328981625E-5	1.359531197388975E-4
4	0.0	0.0	0.0	2.265885328981625E-5	1.359531197388975E-4
5	0.0	0.0	0.0	2.265885328981625E-5	1.5861197302871374E-4
6	0.0	0.0	0.0	2.265885328981625E-5	2.0392967960834626E-4
7	0.0	2.265885328981625E-5	0.0	2.265885328981625E-5	2.0392967960834626E-4
8	0.0	2.265885328981625E-5	0.0	2.265885328981625E-5	2.265885328981625E-4
9	0.0	6.797655986944875E-5	0.0	2.265885328981625E-5	2.265885328981625E-4
10	0.0	6.797655986944875E-5	0.0	6.797655986944875E-5	2.4924738618797875E-4
11	0.0	6.797655986944875E-5	0.0	6.797655986944875E-5	2.4924738618797875E-4
12	0.0	9.0635413159265E-5	0.0	6.797655986944875E-5	7.024244519843037E-4
13	0.0	9.0635413159265E-5	0.0	9.0635413159265E-5	7.2508330527412E-4
14	0.0	9.0635413159265E-5	0.0	9.0635413159265E-5	7.2508330527412E-4
15	0.0	9.0635413159265E-5	0.0	1.8127082631853E-4	9.0635413159265E-4
16	0.0	1.1329426644908125E-4	0.0	2.4924738618797875E-4	9.0635413159265E-4
17	0.0	1.1329426644908125E-4	0.0	2.71906239477795E-4	9.743306914620988E-4
18	0.0	1.1329426644908125E-4	0.0	5.4381247895559E-4	9.743306914620988E-4
19	0.0	1.359531197388975E-4	0.0	7.930598651435688E-4	9.743306914620988E-4
20	0.0	1.359531197388975E-4	0.0	0.0011329426644908125	0.0010423072513315475
21	0.0	1.359531197388975E-4	0.0	0.0012235780776500775	0.0011102838112009962
22	0.0	1.359531197388975E-4	0.0	0.001359531197388975	0.0011329426644908125
23	0.0	1.359531197388975E-4	0.0	0.0018127082631853	0.0011329426644908125
24	0.0	1.359531197388975E-4	0.0	0.0024924738618797874	0.0011329426644908125
25	0.0	1.359531197388975E-4	0.0	0.0032855337270233562	0.0011329426644908125
26	0.0	1.8127082631853E-4	0.0	0.0040106170322974765	0.001178260371070445
27	0.0	1.8127082631853E-4	0.0	0.004916971163890126	0.0012462369309398937
28	0.0	1.8127082631853E-4	0.0	0.005845984148772593	0.00126889578422971
29	0.0	2.265885328981625E-4	0.0	0.0072508330527412	0.00126889578422971
30	0.0	2.265885328981625E-4	0.0	0.008972905902767236	0.00126889578422971
31	0.0	3.3988279934724373E-4	0.0	0.01135208549819794	0.00126889578422971
32	0.0	3.3988279934724373E-4	0.0	0.014909525464699093	0.0013142134908093424
33	0.0	3.3988279934724373E-4	0.0	0.017900494098954837	0.0013368723440991587
34	0.0	3.3988279934724373E-4	0.0	0.02211504081086066	0.0013821900506787912
35	0.0	3.3988279934724373E-4	0.0	0.026216293256317403	0.0013821900506787912
36	0.0	4.3051821250650877E-4	0.0	0.031835688872191834	0.0013821900506787912
37	0.0	4.3051821250650877E-4	0.0	0.041103159867726675	0.0013821900506787912
38	0.0	4.3051821250650877E-4	0.0	0.05290842243172095	0.0014728254638380564
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4005	0.0	26.75813	1
GGTATCA	1690	0.0	23.698124	1
TATCAAC	5945	0.0	17.910172	2
ATCAACG	5985	0.0	17.716957	3
TCAACGC	6025	0.0	17.635847	4
CAACGCA	6210	0.0	17.181314	5
AACGCAG	6490	0.0	16.406158	6
GTATAGA	795	0.0	15.500659	1
GTATAGG	590	0.0	14.172969	1
GGACCGA	875	0.0	13.828048	6
ACGCAGA	7900	0.0	13.533665	7
GTCCTAA	565	0.0	13.2421875	1
AGAGTAC	8205	0.0	13.057545	11
CGCAGAG	8200	0.0	13.011703	8
TATACTG	745	0.0	12.992796	5
GTACATG	9955	0.0	12.798721	1
CAGAGTA	8550	0.0	12.633583	10
AGGACCG	1325	0.0	12.618391	5
CTAGTAC	245	2.2202585E-8	12.570953	3
TTACACG	280	8.367351E-10	12.570953	4
>>END_MODULE