FastQCFastQC Report
Thu 2 Feb 2017
SRR4061975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711909
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54800.1476329295788232No Hit
GCGCAAGACGGACCAGAGCGAAAGC38790.1045014842766889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.014.5926651
GTATCAA43000.013.1835731
CGTCGTA6650.011.71578310
CGCGTAA3350.011.62835210
TCGCGTA3350.011.6283529
CGAGCCG5850.011.53029815
GCGTTAT3800.011.49480151
CGTTATT3500.011.3974592
AAGACGG7800.011.3274555
ACCGTCG6500.011.2548288
CGAACGA1950.011.20504916
ACGGTAT4900.011.0524349
ATACCGT6900.010.8774686
TAACGAA2200.010.79668813
GTTATTC4500.010.76557353
TACCGTC7000.010.7223647
CCGTCGT6750.010.6976639
CAAGACG8400.010.63087754
GCGTAAC3500.010.58778111
CGGTCCA6750.010.55690510
Produced by FastQC (version 0.11.5)