##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061975_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3711909
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256867557906187	32.0	32.0	32.0	32.0	32.0
2	30.75923924859149	32.0	32.0	32.0	32.0	32.0
3	30.78441847577621	32.0	32.0	32.0	32.0	32.0
4	30.803637696937074	32.0	32.0	32.0	32.0	32.0
5	30.703208780172144	32.0	32.0	32.0	32.0	32.0
6	34.36556876798434	36.0	36.0	36.0	32.0	36.0
7	34.295188809854984	36.0	36.0	36.0	32.0	36.0
8	34.253502173679365	36.0	36.0	36.0	32.0	36.0
9	34.39809300281877	36.0	36.0	36.0	32.0	36.0
10	34.05841360873879	36.0	36.0	36.0	32.0	36.0
11	34.38096677477815	36.0	36.0	36.0	32.0	36.0
12	34.18698626501889	36.0	36.0	36.0	32.0	36.0
13	34.289879681856426	36.0	36.0	36.0	32.0	36.0
14	34.18407832735124	36.0	36.0	36.0	32.0	36.0
15	34.13574282128145	36.0	36.0	36.0	32.0	36.0
16	34.14023161666948	36.0	36.0	36.0	32.0	36.0
17	34.06854936368322	36.0	36.0	36.0	32.0	36.0
18	34.074152679928304	36.0	36.0	36.0	32.0	36.0
19	34.0771015668757	36.0	36.0	36.0	32.0	36.0
20	34.065103697315855	36.0	36.0	36.0	32.0	36.0
21	34.04774254972307	36.0	36.0	36.0	32.0	36.0
22	34.01223548314358	36.0	36.0	36.0	32.0	36.0
23	33.982824201778655	36.0	36.0	36.0	32.0	36.0
24	33.96304435264981	36.0	36.0	36.0	32.0	36.0
25	33.607768940456246	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	57.0
5	279.0
6	730.0
7	171.0
8	648.0
9	502.0
10	283.0
11	72.0
12	164.0
13	175.0
14	541.0
15	846.0
16	1312.0
17	1704.0
18	2333.0
19	3206.0
20	4948.0
21	7182.0
22	10821.0
23	16337.0
24	23349.0
25	32737.0
26	44685.0
27	57356.0
28	75915.0
29	99648.0
30	131012.0
31	176907.0
32	249698.0
33	352611.0
34	766412.0
35	1649264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.45426380230089	16.938152081582754	11.828426857348992	25.779157258767366
2	17.0201961150914	18.985037859296934	36.79644559581583	27.19832042979583
3	19.265143269717772	22.625866137913942	27.82894382361499	30.280046768753298
4	13.193845334776046	15.134852888003477	34.56952746659999	37.101774310620485
5	15.507190720850733	35.88022157633968	32.80820118118353	15.80438652162606
6	35.66052002701089	34.19869716938281	16.156393588013852	13.984389215592454
7	31.477865671915506	29.746634030888924	19.70927153103725	19.066228766158325
8	28.809513021827826	32.48911521475066	18.570829854301376	20.130541909120133
9	27.151022693619147	14.53310827978062	18.106966248979788	40.208902777620445
10	16.45467795438783	26.362941616174858	30.48315946131641	26.6992209681209
11	37.50539555923893	21.21965537484029	21.630122543941436	19.64482652197935
12	25.060533014231197	23.12862887867177	27.833020597410137	23.9778175096869
13	29.24601921158122	19.261699327505642	25.00529369129566	26.486987769617475
14	23.983053384589617	19.097011227887904	24.387865822033028	32.53206956548945
15	25.394314533380307	26.713147270644765	21.685775219540197	26.20676297643473
16	26.33405125037593	25.68989490180001	22.727401459479943	25.248652388344123
17	24.590396082173793	25.900303234483175	24.341674754178307	25.16762592916473
18	25.377111026124965	24.96263276762409	25.07013604315666	24.59012016309428
19	25.866167392826778	24.84886008997608	24.780203644832067	24.50476887236508
20	26.152621110830676	24.26393499912633	24.152057001960898	25.4313868880821
21	26.805521437487172	24.058150311430925	24.13327447798007	25.00305377310183
22	26.322872071464687	23.866644193494015	24.508289229732007	25.302194505309288
23	24.884082412908462	24.019806491708426	25.048363591753315	26.047747503629797
24	25.1476801668374	24.581659373103186	24.72060964465349	25.55005081540593
25	25.30377745348964	24.176786290161665	24.622233079763017	25.897203176585677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	318.0
1	318.0
2	708.5
3	1099.0
4	1099.0
5	1099.0
6	2085.5
7	3072.0
8	3072.0
9	3072.0
10	3762.5
11	4453.0
12	4453.0
13	4453.0
14	6058.0
15	7663.0
16	7663.0
17	7663.0
18	12990.5
19	18318.0
20	18318.0
21	18318.0
22	29852.0
23	41386.0
24	41386.0
25	41386.0
26	61680.5
27	81975.0
28	81975.0
29	81975.0
30	105019.5
31	128064.0
32	128064.0
33	128064.0
34	165682.0
35	203300.0
36	203300.0
37	203300.0
38	245447.5
39	287595.0
40	287595.0
41	287595.0
42	336465.0
43	385335.0
44	385335.0
45	385335.0
46	431664.0
47	477993.0
48	477993.0
49	477993.0
50	497806.5
51	517620.0
52	517620.0
53	517620.0
54	492865.0
55	468110.0
56	468110.0
57	468110.0
58	433423.5
59	398737.0
60	398737.0
61	398737.0
62	349906.5
63	301076.0
64	301076.0
65	301076.0
66	245635.5
67	190195.0
68	190195.0
69	190195.0
70	144116.5
71	98038.0
72	98038.0
73	98038.0
74	73552.0
75	49066.0
76	49066.0
77	49066.0
78	39037.5
79	29009.0
80	29009.0
81	29009.0
82	20001.5
83	10994.0
84	10994.0
85	10994.0
86	7912.5
87	4831.0
88	4831.0
89	4831.0
90	3287.0
91	1743.0
92	1743.0
93	1743.0
94	1099.5
95	456.0
96	456.0
97	456.0
98	959.5
99	1463.0
100	1463.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012392545183623844
2	1.8858220931601503E-4
3	7.812691528806336E-4
4	0.010318140881147679
5	0.03297494631468605
6	0.06180108402441978
7	0.1054174550076524
8	0.1072493964695794
9	0.11710955198524532
10	0.13125321768394643
11	0.13893120763466993
12	0.13041806789983268
13	0.1257035126669323
14	0.11492738642030287
15	0.1304719485310658
16	0.1191839562877215
17	0.12249761510856004
18	0.11085939876220026
19	0.09671573306349913
20	0.09173177467443301
21	0.09111214741525184
22	0.1019421542931144
23	0.08146751442451848
24	0.09127378930895126
25	0.08817565301304531
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3711909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.23682432093298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09356272097047	32.61283831478705
2	14.741797639428905	13.337442199791798
3	5.241361444458477	7.11307640596439
4	2.366385537949234	4.28191067423224
5	1.3931131076975378	3.151000645605126
6	0.8711027635258375	2.364355360745852
7	0.5853595597158473	1.8535865297211125
8	0.4312900882881347	1.5608155164200022
9	0.310864942971154	1.265628853145065
>10	1.7635579643817165	14.511743934452332
>50	0.11723809512047788	3.622468349316257
>100	0.06842543272417029	6.2407943713706535
>500	0.00982810246583401	3.1753096266592973
>1k	0.006052672848114781	4.760470379501972
>5k	5.992745394173051E-5	0.14855883828676505
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5480	0.1476329295788232	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3879	0.1045014842766889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6940315616573573E-5
2	0.0	0.0	0.0	0.0	2.6940315616573573E-5
3	0.0	0.0	0.0	0.0	2.6940315616573573E-5
4	0.0	0.0	0.0	0.0	2.6940315616573573E-5
5	0.0	0.0	0.0	0.0	2.6940315616573573E-5
6	0.0	0.0	0.0	0.0	1.6164189369944146E-4
7	0.0	0.0	0.0	8.082094684972073E-5	1.6164189369944146E-4
8	0.0	0.0	0.0	8.082094684972073E-5	1.6164189369944146E-4
9	0.0	0.0	0.0	1.0776126246629429E-4	1.6164189369944146E-4
10	0.0	0.0	0.0	1.3470157808286788E-4	1.6164189369944146E-4
11	0.0	0.0	0.0	1.3470157808286788E-4	1.6164189369944146E-4
12	0.0	0.0	0.0	1.3470157808286788E-4	4.3104504986517716E-4
13	0.0	0.0	0.0	1.3470157808286788E-4	5.388063123314715E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1360	0.0	14.592665	1
GTATCAA	4300	0.0	13.183573	1
CGTCGTA	665	0.0	11.715783	10
CGCGTAA	335	0.0	11.628352	10
TCGCGTA	335	0.0	11.628352	9
CGAGCCG	585	0.0	11.530298	15
GCGTTAT	380	0.0	11.4948015	1
CGTTATT	350	0.0	11.397459	2
AAGACGG	780	0.0	11.327455	5
ACCGTCG	650	0.0	11.254828	8
CGAACGA	195	0.0	11.205049	16
ACGGTAT	490	0.0	11.052434	9
ATACCGT	690	0.0	10.877468	6
TAACGAA	220	0.0	10.796688	13
GTTATTC	450	0.0	10.7655735	3
TACCGTC	700	0.0	10.722364	7
CCGTCGT	675	0.0	10.697663	9
CAAGACG	840	0.0	10.6308775	4
GCGTAAC	350	0.0	10.587781	11
CGGTCCA	675	0.0	10.556905	10
>>END_MODULE