FastQCFastQC Report
Thu 2 Feb 2017
SRR4061975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711909
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT39380.10609096289806673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41800.028.7431071
GGTATCA17050.025.6830161
TCAACGC63000.018.6469124
CAACGCA64050.018.3412255
ATCAACG64750.018.142943
GTATTAT4400.018.0039251
AACGCAG65700.017.8471156
TATCAAC67650.017.6578662
ATACCGT9250.017.1236746
CGTCGTA9450.016.76126910
TACCGTC9500.016.6730527
ACCGTCG9300.015.8488618
TACGAAT8050.015.30500441
ATACGAA8300.015.10908140
TACGACG11100.015.0624925
CGACGGT10450.014.9468017
GGCGTTA13550.014.93871542
ACGCAGA78850.014.870717
CGCAATA8700.014.41402336
TATACCG1851.2684177E-714.2697295
Produced by FastQC (version 0.11.5)