FastQCFastQC Report
Thu 2 Feb 2017
SRR4061974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006273
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38870.12929630808645787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.012.4128411
GTATCAA26550.012.1255651
CGTCGTA2450.011.2460810
CGAACGA855.3288873E-511.1759716
CGTTATT951.3636893E-510.9982262
GTCTACG700.001496604310.8532241
GCGTAAC1252.2386303E-710.64129611
ACCGTCG2900.010.4838618
CGGTCGG1659.295036E-1010.36472610
CCGTCGT2500.010.261089
TACCGTC2950.09.9842677
GTCTACC1252.5838744E-69.8764341
ACGGTAT2151.0913936E-119.7219529
GTATAAG2650.09.6757521
ATACCGT2950.09.6621946
CCGATAA1201.6993981E-59.5009999
CGCGTAA1304.2359534E-69.50099910
CCGATCC1804.1909516E-99.4994173
GCGTTAT1106.867464E-59.4965711
GACCGCG3850.09.3777667
Produced by FastQC (version 0.11.5)