FastQCFastQC Report
Thu 2 Feb 2017
SRR4061974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006273
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31550.026.785851
GGTATCA17850.021.2062451
TATCAAC48000.017.3716682
CGGATCG3050.017.31144326
TCAACGC49150.016.9190394
ATCAACG49900.016.6650223
CAACGCA51050.016.289345
TATGCGA1501.840981E-716.13276717
AACGCAG52900.015.7196736
GGCGTTA4350.015.67917442
ATTAGAC2900.014.4135273
GTATAGG4600.014.3528121
TATACCG1402.760242E-514.142365
CGATTTT3900.014.102303531
ATCGATT3650.013.86275729
ATACCGT4200.013.6185686
ACCGATA1302.3330118E-413.5379868
ACGCAGA62150.013.380067
GGATCGA3800.013.31576427
CGGCGTT5300.013.28387841
Produced by FastQC (version 0.11.5)