##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006273
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265571356959263	32.0	32.0	32.0	32.0	32.0
2	31.4133300601775	32.0	32.0	32.0	32.0	32.0
3	31.546011623029578	32.0	32.0	32.0	32.0	32.0
4	31.630123411945622	32.0	32.0	32.0	32.0	32.0
5	31.596116852993724	32.0	32.0	32.0	32.0	32.0
6	35.23725889165755	36.0	36.0	36.0	36.0	36.0
7	35.23982319636307	36.0	36.0	36.0	36.0	36.0
8	35.19064003834649	36.0	36.0	36.0	36.0	36.0
9	35.2863236306217	36.0	36.0	36.0	36.0	36.0
10	35.16059419753296	36.0	36.0	36.0	36.0	36.0
11	35.28391599831419	36.0	36.0	36.0	36.0	36.0
12	35.210170200776844	36.0	36.0	36.0	36.0	36.0
13	35.240310843359865	36.0	36.0	36.0	36.0	36.0
14	35.20676964467299	36.0	36.0	36.0	36.0	36.0
15	35.17750749848733	36.0	36.0	36.0	36.0	36.0
16	35.19782168818334	36.0	36.0	36.0	36.0	36.0
17	35.17926216281755	36.0	36.0	36.0	36.0	36.0
18	35.17253156982084	36.0	36.0	36.0	36.0	36.0
19	35.165595074033526	36.0	36.0	36.0	36.0	36.0
20	35.16798241543599	36.0	36.0	36.0	36.0	36.0
21	35.16233522371388	36.0	36.0	36.0	36.0	36.0
22	35.14026437386092	36.0	36.0	36.0	36.0	36.0
23	35.10680067977858	36.0	36.0	36.0	36.0	36.0
24	35.09165934031939	36.0	36.0	36.0	36.0	36.0
25	35.08427311824309	36.0	36.0	36.0	36.0	36.0
26	35.03854639947869	36.0	36.0	36.0	36.0	36.0
27	35.02657376758531	36.0	36.0	36.0	36.0	36.0
28	35.00709050708302	36.0	36.0	36.0	36.0	36.0
29	34.989319333274125	36.0	36.0	36.0	36.0	36.0
30	34.972058425831584	36.0	36.0	36.0	36.0	36.0
31	34.9678352564787	36.0	36.0	36.0	36.0	36.0
32	34.93743249531895	36.0	36.0	36.0	36.0	36.0
33	34.916220516233885	36.0	36.0	36.0	32.0	36.0
34	34.90262926886547	36.0	36.0	36.0	32.0	36.0
35	34.894492283302284	36.0	36.0	36.0	32.0	36.0
36	34.87336645740424	36.0	36.0	36.0	32.0	36.0
37	34.862059433724085	36.0	36.0	36.0	32.0	36.0
38	34.85239331225075	36.0	36.0	36.0	32.0	36.0
39	34.83381615708221	36.0	36.0	36.0	32.0	36.0
40	34.82737695478754	36.0	36.0	36.0	32.0	36.0
41	34.798342998124255	36.0	36.0	36.0	32.0	36.0
42	34.77952567847298	36.0	36.0	36.0	32.0	36.0
43	34.76542183627369	36.0	36.0	36.0	32.0	36.0
44	34.729566476497645	36.0	36.0	36.0	32.0	36.0
45	34.72342066073174	36.0	36.0	36.0	32.0	36.0
46	34.70322522272595	36.0	36.0	36.0	32.0	36.0
47	34.679674467355426	36.0	36.0	36.0	32.0	36.0
48	34.66128625045031	36.0	36.0	36.0	32.0	36.0
49	34.64721866576988	36.0	36.0	36.0	32.0	36.0
50	34.224888092332264	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	9.0
20	19.0
21	85.0
22	197.0
23	547.0
24	1330.0
25	3347.0
26	7376.0
27	13688.0
28	23320.0
29	37085.0
30	56474.0
31	84012.0
32	127529.0
33	217438.0
34	495864.0
35	1937949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.899798192214845	17.6165718630369	12.355328108180952	26.128301836567303
2	16.35908990054665	19.65002879114131	36.83280147618219	27.15807983212985
3	18.956890997228122	22.81457078106606	28.150996769078418	30.0775414526274
4	13.255183411486582	16.105955779797775	34.283712756625896	36.355148052089746
5	15.202145646785903	35.99184106034282	32.88430558369117	15.921707709180104
6	35.47361712262513	33.943780128717734	16.533655682061614	14.048947066595527
7	31.354404606634194	29.6467087320413	19.786493109574547	19.212393551749958
8	28.61207215711946	33.20925943851407	18.50966296141435	19.66900544295212
9	27.109048313310204	14.938862837806147	18.421347628774896	39.53074122010875
10	17.11016168862964	26.868926031975814	30.621780065143806	25.39913221425074
11	36.74014302759596	21.392435084904132	21.75906845452825	20.108353432971658
12	24.644534944098556	23.389292988361337	28.348090808785496	23.61808125875461
13	29.862856766501245	19.714210918303163	24.657341498925746	25.765590816269846
14	23.933588200406284	18.93437488877424	24.88925656452358	32.242780346295895
15	25.544652797666746	26.69474794870592	21.794494378920344	25.966104874706986
16	25.98452489100197	25.72719407287558	23.221411946523357	25.06686908959909
17	24.596568575109444	25.721616100733367	24.677266502410127	25.004548821747058
18	24.97021225638416	25.379224088322072	25.145745392130447	24.504818263163322
19	25.940425237495063	24.87658306481148	24.936624185494797	24.24636751219866
20	26.114266193121622	24.535694006608207	24.629930668029157	24.720109132241014
21	26.39224714455407	24.41917284291879	24.4509064878672	24.737673524659936
22	26.249612061180073	24.107990192507465	24.59197817363892	25.050419572673537
23	25.241794488323077	24.504003629744748	24.837839024546014	25.41636285738616
24	25.111251559251556	24.61738045738046	24.821987525987527	25.449380457380457
25	25.312987835079188	24.411837005828158	24.91555141309804	25.359623745994618
26	25.29043533450001	24.873805119512166	24.887576369732123	24.948183176255704
27	25.52439724960674	24.812815653730663	24.631028377110304	25.031758719552293
28	25.483670416544836	24.47573057426392	24.92283527114184	25.117763738049405
29	25.222926715846317	24.772765601876355	24.830777940808122	25.17352974146921
30	25.34421690321877	24.682959866198487	24.88254446412201	25.090278766460738
31	25.466518664239285	24.634986296913404	24.60142296550162	25.297072073345696
32	25.552208818642523	24.68691008217893	24.553454558760183	25.207426540418364
33	25.31989829250087	24.71133644771524	24.547212690285765	25.42155256949813
34	25.465080462263	24.598353964768112	24.959866997266367	24.976698575702518
35	25.518791935369595	24.63630094905203	25.00965484390421	24.835252271674168
36	25.374634180741158	24.694687695633057	24.757157217624787	25.173520906001002
37	25.617279946563997	24.644594378375718	24.731747218177823	25.006378456882462
38	25.417119469855198	24.689940002122228	24.809190649019566	25.083749879003005
39	25.605838240890304	24.743234756413976	24.575184748563657	25.07574225413206
40	25.86501855292198	24.47931236933537	24.552958571516484	25.102710506226167
41	25.191269082001543	24.803342119514234	24.794161203578163	25.211227594906056
42	25.552240096869056	24.674863278911022	24.787300573496733	24.985596050723192
43	25.604113178951625	24.276963629795265	24.692479596772205	25.4264435944809
44	25.129651399902464	24.687855314692804	24.94183567088404	25.24065761452069
45	25.37584662164657	24.68960116931169	24.675264194519816	25.259288014521925
46	25.409329734099085	24.7558125803549	24.61270809112516	25.222149594420856
47	25.388748570061892	24.73321404847037	24.842453170046795	25.035584211420943
48	25.49804269089419	25.139446631059876	24.39278177400598	24.96972890403996
49	25.455281518427814	24.846132710080163	24.510588584381015	25.187997187111005
50	25.36477799968864	25.015734086617975	24.368707196964152	25.250780716729228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	31.0
2	50.0
3	138.5
4	227.0
5	259.5
6	292.0
7	268.0
8	244.0
9	309.0
10	374.0
11	612.5
12	851.0
13	1649.0
14	2447.0
15	3046.5
16	3646.0
17	3670.0
18	3694.0
19	3747.0
20	3800.0
21	4512.5
22	5225.0
23	6764.0
24	8303.0
25	11128.5
26	13954.0
27	17479.0
28	21004.0
29	25439.0
30	29874.0
31	36235.5
32	42597.0
33	49047.0
34	55497.0
35	65724.5
36	75952.0
37	86835.5
38	97719.0
39	109688.0
40	121657.0
41	133167.5
42	144678.0
43	155120.0
44	165562.0
45	179313.0
46	193064.0
47	206128.0
48	219192.0
49	229226.5
50	239261.0
51	241999.0
52	244737.0
53	244376.0
54	244015.0
55	242535.5
56	241056.0
57	232198.5
58	223341.0
59	204011.5
60	184682.0
61	161188.5
62	137695.0
63	118660.5
64	99626.0
65	83146.0
66	66666.0
67	55094.5
68	43523.0
69	35869.0
70	28215.0
71	22016.0
72	15817.0
73	13286.5
74	10756.0
75	8555.0
76	6354.0
77	5381.5
78	4409.0
79	3642.0
80	2875.0
81	2281.5
82	1688.0
83	1263.5
84	839.0
85	625.0
86	411.0
87	283.0
88	155.0
89	108.5
90	62.0
91	54.5
92	47.0
93	48.5
94	50.0
95	40.0
96	30.0
97	34.0
98	38.0
99	24.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03120142448806213
2	0.004656929028068975
3	3.3263778771921244E-4
4	0.0
5	0.0
6	1.6631889385960622E-4
7	0.0
8	0.0
9	0.0
10	3.326377877192125E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.648582480699523E-4
17	0.0
18	9.979133631576374E-5
19	0.0
20	3.9916534526305495E-4
21	0.0
22	0.0
23	3.326377877192125E-5
24	7.650669117541885E-4
25	1.9958267263152748E-4
26	3.3263778771921244E-4
27	3.9916534526305495E-4
28	0.0013970787084206923
29	5.3222046035074E-4
30	9.646495843857161E-4
31	5.3222046035074E-4
32	9.313858056137949E-4
33	1.6631889385960622E-4
34	6.320117966665037E-4
35	3.3263778771921244E-4
36	2.3284645140344873E-4
37	0.001995826726315275
38	0.0
39	8.315944692980312E-4
40	2.6611023017537E-4
41	0.001230759814561086
42	0.0050893581521039505
43	0.004357555019121683
44	0.004490610134209368
45	0.001995826726315275
46	0.002594574744209857
47	7.983306905261099E-4
48	0.0034926967710517307
49	0.0036922794436832587
50	0.0020290905050871963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3006273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.88293486745856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.67755304030164	27.30680359546393
2	16.666369349445556	14.29405662578563
3	7.3442401701949365	9.448277186083267
4	3.902758941589366	6.694470299822733
5	2.302963154453097	4.937890947728453
6	1.4395123129282472	3.703830765372399
7	0.9802839420181999	2.9426216706027395
8	0.6876464742903389	2.3590639175064094
9	0.5028692765530427	1.9408059389943122
>10	2.3197671332395386	17.129766874223787
>50	0.11392588511090133	3.3200882958874476
>100	0.05805931407170094	4.635633886565774
>500	0.0033498701839044342	0.9275927412525758
>1k	7.011356197638573E-4	0.3590972547105142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.33055115087685E-4
2	0.0	0.0	0.0	0.0	1.33055115087685E-4
3	0.0	0.0	0.0	0.0	1.33055115087685E-4
4	0.0	0.0	0.0	0.0	1.6631889385960622E-4
5	0.0	0.0	0.0	0.0	1.9958267263152748E-4
6	0.0	0.0	0.0	0.0	1.9958267263152748E-4
7	0.0	0.0	0.0	0.0	1.9958267263152748E-4
8	0.0	0.0	0.0	0.0	1.9958267263152748E-4
9	0.0	0.0	0.0	0.0	1.9958267263152748E-4
10	0.0	0.0	0.0	0.0	1.9958267263152748E-4
11	0.0	0.0	0.0	0.0	2.3284645140344873E-4
12	0.0	0.0	0.0	6.65275575438425E-5	6.320117966665036E-4
13	0.0	0.0	0.0	6.65275575438425E-5	8.648582480699524E-4
14	0.0	0.0	0.0	9.979133631576374E-5	8.981220268418737E-4
15	0.0	0.0	0.0	3.659015664911337E-4	9.979133631576374E-4
16	0.0	0.0	0.0	4.324291240349762E-4	0.0010977046994734012
17	0.0	0.0	0.0	5.987480178945824E-4	0.0010977046994734012
18	0.0	0.0	0.0	8.315944692980312E-4	0.0010977046994734012
19	0.0	0.0	0.0	0.0011974960357891648	0.0010977046994734012
20	0.0	0.0	0.0	0.0014636062659645348	0.0012972873721049285
21	0.0	0.0	0.0	0.0016964527173679836	0.0014303424871926135
22	0.0	0.0	0.0	0.0020956180626310384	0.0014636062659645348
23	0.0	0.0	0.0	0.002960476310700991	0.0014636062659645348
24	0.0	0.0	0.0	0.004124708567718235	0.0014636062659645348
25	0.0	0.0	0.0	0.0053554683822793206	0.0014636062659645348
26	0.0	0.0	0.0	0.006785810869471934	0.001496870044736456
27	0.0	0.0	0.0	0.007816988011401493	0.0015633976022802986
28	0.0	0.0	0.0	0.009114275383506421	0.0016631889385960623
29	0.0	0.0	0.0	0.010877255658418248	0.0016964527173679836
30	0.0	0.0	0.0	0.012440653260698546	0.0016964527173679836
31	0.0	0.0	0.0	0.014735853995961112	0.0017297164961399048
32	0.0	0.0	0.0	0.01842813343964437	0.001762980274911826
33	0.0	0.0	0.0	0.021987357768239943	0.0017962440536837473
34	0.0	0.0	0.0	0.026245121451045864	0.0018960353899995111
35	0.0	0.0	0.0	0.030336566239992178	0.0018960353899995111
36	0.0	0.0	0.0	0.03575856217981534	0.0018960353899995111
37	0.0	0.0	0.0	0.04434061710297102	0.0018960353899995111
38	0.0	0.0	0.0	0.055217872761389265	0.0019292991687714324
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3155	0.0	26.78585	1
GGTATCA	1785	0.0	21.206245	1
TATCAAC	4800	0.0	17.371668	2
CGGATCG	305	0.0	17.311443	26
TCAACGC	4915	0.0	16.919039	4
ATCAACG	4990	0.0	16.665022	3
CAACGCA	5105	0.0	16.28934	5
TATGCGA	150	1.840981E-7	16.132767	17
AACGCAG	5290	0.0	15.719673	6
GGCGTTA	435	0.0	15.679174	42
ATTAGAC	290	0.0	14.413527	3
GTATAGG	460	0.0	14.352812	1
TATACCG	140	2.760242E-5	14.14236	5
CGATTTT	390	0.0	14.1023035	31
ATCGATT	365	0.0	13.862757	29
ATACCGT	420	0.0	13.618568	6
ACCGATA	130	2.3330118E-4	13.537986	8
ACGCAGA	6215	0.0	13.38006	7
GGATCGA	380	0.0	13.315764	27
CGGCGTT	530	0.0	13.283878	41
>>END_MODULE