FastQCFastQC Report
Thu 2 Feb 2017
SRR4061973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101285
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1960.1935133534086982No Hit
TATCAACGCAGAGTACTTTTTTTTT1640.16191933652564544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG250.005994025619.0000025
AGGGCTC350.002151394716.28571512
GAGGGCT456.685343E-414.77777911
GGGCTCA400.005234091514.2513
GTATCAA801.9472845E-613.06251
ACTGAGG657.9102605E-411.6923078
TCAACGC1106.9983344E-48.6363644
TATCAAC1252.6766717E-48.362
CAACGCA1200.00165696977.9166675
AACGCAG1200.00165696977.9166676
ATCAACG1250.00247551487.63
AGAGTAC1650.005106266.333333511
Produced by FastQC (version 0.11.5)