##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061973_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101285
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.148304289875107	32.0	32.0	32.0	21.0	32.0
2	28.693636767537146	32.0	32.0	32.0	14.0	32.0
3	28.822076319297032	32.0	32.0	32.0	14.0	32.0
4	28.708061410870318	32.0	32.0	32.0	14.0	32.0
5	28.61083082391272	32.0	32.0	32.0	14.0	32.0
6	31.827536160339637	36.0	32.0	36.0	21.0	36.0
7	31.850323345016538	36.0	32.0	36.0	21.0	36.0
8	31.568307251814186	36.0	32.0	36.0	21.0	36.0
9	32.06357308584687	36.0	32.0	36.0	21.0	36.0
10	31.021967714863997	36.0	32.0	36.0	14.0	36.0
11	32.30118971219825	36.0	32.0	36.0	21.0	36.0
12	31.523799180530187	36.0	32.0	36.0	21.0	36.0
13	31.93169768475095	36.0	32.0	36.0	21.0	36.0
14	31.530295700251767	36.0	32.0	36.0	21.0	36.0
15	31.35629165226835	36.0	32.0	36.0	14.0	36.0
16	31.40971516019154	36.0	32.0	36.0	14.0	36.0
17	31.289519672212077	36.0	32.0	36.0	14.0	36.0
18	31.259041319050205	36.0	32.0	36.0	14.0	36.0
19	31.278757960211284	36.0	32.0	36.0	14.0	36.0
20	31.278353161869973	36.0	32.0	36.0	14.0	36.0
21	31.236609567063237	36.0	32.0	36.0	14.0	36.0
22	31.122871106284247	36.0	32.0	36.0	14.0	36.0
23	31.07908377351039	36.0	32.0	36.0	14.0	36.0
24	30.988685392703758	36.0	32.0	36.0	14.0	36.0
25	30.05178456829738	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	8.0
6	24.0
7	6.0
8	17.0
9	9.0
10	4.0
11	1.0
12	2.0
13	1.0
14	66.0
15	106.0
16	162.0
17	221.0
18	343.0
19	444.0
20	698.0
21	939.0
22	1265.0
23	1608.0
24	2242.0
25	2885.0
26	3644.0
27	4735.0
28	6106.0
29	7220.0
30	8728.0
31	10425.0
32	12444.0
33	13521.0
34	15082.0
35	8325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4759290707318	17.053038979503178	11.84787330674144	26.623158643023576
2	16.754702078293924	18.66811472577381	36.619440193513356	27.957743002418916
3	19.206389953003438	21.890920579755935	28.39639034793255	30.506299119308082
4	13.525282146983027	15.189036010150382	33.964276193015195	37.3214056498514
5	15.416979417690516	35.61509896100818	33.2615652036503	15.70635641765101
6	35.2426867942423	33.68569763191432	16.206123235297024	14.86549233854635
7	30.931696662416858	29.43042666113203	19.898992893922774	19.73888378252834
8	28.471041707847398	32.82763391974699	18.34552283059893	20.35580154180668
9	26.68030680425414	14.551456924840863	18.27580753568181	40.492428735223186
10	16.809347753020027	26.293521026512977	31.31141382787323	25.585717392593764
11	36.03199240724081	21.360567084202515	21.785682507983275	20.82175800057341
12	24.333755090743743	23.10209165315725	28.383614724605593	24.18053853149342
13	29.515148519744972	19.534423960856024	24.294963673207135	26.655463846191864
14	23.785851238362554	18.49637287264533	25.114150738273604	32.60362515071851
15	25.183618192781804	26.211682367710875	22.294164747284032	26.310534692223285
16	25.548527376951967	25.798576793832773	23.08163668709231	25.571259142122948
17	24.406927091570456	25.466550687964574	24.486991934208447	25.639530286256523
18	24.69658035184819	25.51690057323582	25.048428543190354	24.738090531725636
19	25.62354243250721	24.684770149017748	25.14130993319894	24.550377485276098
20	25.75943237741368	24.62300136371722	24.81767693736783	24.799889321501276
21	25.262117693561937	24.22846978605662	24.886605069420426	25.622807450961016
22	25.50157142574766	23.711727382340733	24.948113300784726	25.83858789112688
23	25.057553032773118	24.370868779085278	24.45682781515843	26.114750372983174
24	24.799162063615974	24.434541160660466	25.03829013547297	25.72800664025059
25	25.10054645888712	24.69934879492475	24.8070594978112	25.39304524837693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	17.0
3	30.0
4	30.0
5	30.0
6	59.0
7	88.0
8	88.0
9	88.0
10	108.5
11	129.0
12	129.0
13	129.0
14	187.5
15	246.0
16	246.0
17	246.0
18	363.0
19	480.0
20	480.0
21	480.0
22	765.0
23	1050.0
24	1050.0
25	1050.0
26	1610.0
27	2170.0
28	2170.0
29	2170.0
30	2904.5
31	3639.0
32	3639.0
33	3639.0
34	4337.0
35	5035.0
36	5035.0
37	5035.0
38	6338.5
39	7642.0
40	7642.0
41	7642.0
42	9165.0
43	10688.0
44	10688.0
45	10688.0
46	12166.0
47	13644.0
48	13644.0
49	13644.0
50	13975.5
51	14307.0
52	14307.0
53	14307.0
54	13861.0
55	13415.0
56	13415.0
57	13415.0
58	12241.0
59	11067.0
60	11067.0
61	11067.0
62	9515.0
63	7963.0
64	7963.0
65	7963.0
66	6529.0
67	5095.0
68	5095.0
69	5095.0
70	3909.0
71	2723.0
72	2723.0
73	2723.0
74	1931.0
75	1139.0
76	1139.0
77	1139.0
78	796.5
79	454.0
80	454.0
81	454.0
82	308.5
83	163.0
84	163.0
85	163.0
86	111.5
87	60.0
88	60.0
89	60.0
90	38.0
91	16.0
92	16.0
93	16.0
94	11.0
95	6.0
96	6.0
97	6.0
98	19.0
99	32.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.873130275953991E-4
2	0.0
3	9.873130275953991E-4
4	0.007898504220763193
5	0.032581329910648174
6	0.06318803376610554
7	0.10268055486992152
8	0.10366786789751692
9	0.1115663721182801
10	0.12538875450461567
11	0.13427457175297428
12	0.11946487633904329
13	0.11847756331144789
14	0.1016932418423261
15	0.1224268154218295
16	0.10366786789751692
17	0.11551562422866171
18	0.10366786789751692
19	0.08787085945599052
20	0.08984548551118132
21	0.08885817248358592
22	0.1016932418423261
23	0.07306116404205953
24	0.08293429431801352
25	0.08688354642839512
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	101285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.194155106876636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.636889608886825	32.578367971565385
2	16.97909434544473	17.384607789899786
3	7.822263364961815	12.013624919780815
4	4.0442027308493405	8.281581675470209
5	2.4029931343053303	6.150960161919337
6	1.4811386253182135	4.549538431159599
7	1.025997068579804	3.6767537147652662
8	0.7540692740877883	3.0883151503184085
9	0.491784309187688	2.265883398331441
>10	1.3519247087865462	9.451547613170757
>50	0.0057856977551492715	0.2033864836846522
>100	0.0038571318367661804	0.3554326899343437
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	196	0.1935133534086982	No Hit
TATCAACGCAGAGTACTTTTTTTTT	164	0.16191933652564544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	25	0.0059940256	19.000002	5
AGGGCTC	35	0.0021513947	16.285715	12
GAGGGCT	45	6.685343E-4	14.777779	11
GGGCTCA	40	0.0052340915	14.25	13
GTATCAA	80	1.9472845E-6	13.0625	1
ACTGAGG	65	7.9102605E-4	11.692307	8
TCAACGC	110	6.9983344E-4	8.636364	4
TATCAAC	125	2.6766717E-4	8.36	2
CAACGCA	120	0.0016569697	7.916667	5
AACGCAG	120	0.0016569697	7.916667	6
ATCAACG	125	0.0024755148	7.6	3
AGAGTAC	165	0.00510626	6.3333335	11
>>END_MODULE