FastQCFastQC Report
Thu 2 Feb 2017
SRR4061973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101285
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCAA250.002342658435.211
CATAATC250.002342658435.22
TTAGGCT300.005720504629.3333364
GCGCGGG300.005720504629.33333613
ATAATCC300.005720504629.3333363
TATAACA300.005720504629.3333364
ATCAACG852.3087523E-723.2941192
TCAACGC904.0059967E-721.9999983
GTCCAAG550.004455787620.01
GTATCAA808.8854E-519.251
AACGCAG1102.745459E-618.05
ACGCAGA1351.0615968E-616.2962956
TATCAAC1259.270594E-615.8399981
CAACGCA1259.270594E-615.8399984
ATGGGGG850.002954795515.52941217
CGCAGAG1452.2356799E-615.1724157
GAGTACA1606.2058534E-613.7511
AGAGTAC1453.768203E-513.65517310
GCAGAGT1453.768203E-513.6551738
AGTACAT1353.1622127E-413.03703712
Produced by FastQC (version 0.11.5)