##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101285
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88367477908871	32.0	32.0	32.0	32.0	32.0
2	30.552618847805697	32.0	32.0	32.0	32.0	32.0
3	31.129041812706717	32.0	32.0	32.0	32.0	32.0
4	31.396218591104308	32.0	32.0	32.0	32.0	32.0
5	31.261104803277878	32.0	32.0	32.0	32.0	32.0
6	34.95767389050698	36.0	36.0	36.0	36.0	36.0
7	34.93531125043195	36.0	36.0	36.0	36.0	36.0
8	34.88640963617515	36.0	36.0	36.0	32.0	36.0
9	35.047213308979615	36.0	36.0	36.0	36.0	36.0
10	34.766855901663625	36.0	36.0	36.0	32.0	36.0
11	35.08873969492027	36.0	36.0	36.0	36.0	36.0
12	34.96284741077159	36.0	36.0	36.0	32.0	36.0
13	35.01837389544355	36.0	36.0	36.0	36.0	36.0
14	34.977538628622206	36.0	36.0	36.0	32.0	36.0
15	34.9011699659377	36.0	36.0	36.0	32.0	36.0
16	34.93152984153626	36.0	36.0	36.0	32.0	36.0
17	34.94393049316286	36.0	36.0	36.0	32.0	36.0
18	34.874236066544896	36.0	36.0	36.0	32.0	36.0
19	34.92575406032483	36.0	36.0	36.0	32.0	36.0
20	34.89050698523967	36.0	36.0	36.0	32.0	36.0
21	34.8610949301476	36.0	36.0	36.0	32.0	36.0
22	34.900103667867896	36.0	36.0	36.0	32.0	36.0
23	34.83654045515131	36.0	36.0	36.0	32.0	36.0
24	34.840864886212174	36.0	36.0	36.0	32.0	36.0
25	34.82699313817446	36.0	36.0	36.0	32.0	36.0
26	34.748008095966824	36.0	36.0	36.0	32.0	36.0
27	34.74751443945303	36.0	36.0	36.0	32.0	36.0
28	34.72164683813003	36.0	36.0	36.0	32.0	36.0
29	34.686537986868736	36.0	36.0	36.0	32.0	36.0
30	34.675371476526635	36.0	36.0	36.0	32.0	36.0
31	34.638051044083525	36.0	36.0	36.0	32.0	36.0
32	34.61237103223577	36.0	36.0	36.0	32.0	36.0
33	34.58946536999556	36.0	36.0	36.0	32.0	36.0
34	34.58551611788518	36.0	36.0	36.0	32.0	36.0
35	34.58184331342252	36.0	36.0	36.0	32.0	36.0
36	34.51396554277534	36.0	36.0	36.0	32.0	36.0
37	34.51750012341413	36.0	36.0	36.0	32.0	36.0
38	34.538401540208326	36.0	36.0	36.0	32.0	36.0
39	34.49360714814632	36.0	36.0	36.0	32.0	36.0
40	34.46213160882658	36.0	36.0	36.0	32.0	36.0
41	34.453374142271805	36.0	36.0	36.0	32.0	36.0
42	34.414098830034064	36.0	36.0	36.0	32.0	36.0
43	34.40540060226095	36.0	36.0	36.0	32.0	36.0
44	34.3720392950585	36.0	36.0	36.0	32.0	36.0
45	34.338352174556945	36.0	36.0	36.0	32.0	36.0
46	34.32874561879844	36.0	36.0	36.0	32.0	36.0
47	34.25984104260256	36.0	36.0	36.0	32.0	36.0
48	34.24405390729131	36.0	36.0	36.0	32.0	36.0
49	34.22129634200523	36.0	36.0	36.0	32.0	36.0
50	33.65569432788666	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	3.0
23	11.0
24	35.0
25	84.0
26	208.0
27	431.0
28	772.0
29	1367.0
30	2224.0
31	3801.0
32	6620.0
33	12214.0
34	26432.0
35	47081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08046294833307	17.44647653657766	12.578013904250277	26.895046610838996
2	16.56644647846994	19.285721338507262	35.99435212338438	28.15348005963842
3	18.969245199190404	21.890704447845188	28.222342893814485	30.917707459149923
4	13.23789307399911	16.029027003011304	34.26963518783631	36.46344473515328
5	15.51266228957891	35.457372760033564	32.95255960902404	16.07740534136348
6	35.73974428592585	33.76314360467986	15.945105395665696	14.552006713728588
7	30.909808954929158	29.529545342350794	19.695907587500617	19.86473811521943
8	28.436589820802688	32.5102433726613	18.569383423014266	20.483783383521743
9	26.846028533346498	14.341709038850766	17.77064718368959	41.041615244113146
10	16.4594954830429	26.4590018265291	31.586118378832005	25.49538431159599
11	36.85836994619144	20.975465271264255	21.690279903243322	20.47588487930098
12	24.517944414276545	22.776324233598263	28.38228760428494	24.323443747840255
13	29.797107172829147	19.40958680949795	24.290862417929603	26.502443599743298
14	23.923581971664117	18.219874611245494	24.804265192279214	33.052278224811175
15	25.238682924421184	26.372118280100704	21.947968603445723	26.441230192032382
16	25.643000306073084	25.87601078167116	23.071986414304472	25.409002497951285
17	25.020486745322607	25.15476131707558	24.4636421977588	25.36110973984302
18	25.045169571012487	25.455891790492174	24.874364417238485	24.62457422125685
19	25.911043096213653	24.83684652218986	24.84770696549341	24.404403416103076
20	26.052228859159797	24.458705632620823	24.599891395566964	24.889174112652416
21	25.29890901910451	24.46759144986918	24.9790195981636	25.254479932862715
22	25.610899935824655	23.992693883595795	24.666041368415854	25.730364812163696
23	25.511433197741006	24.607045535326407	23.868528099206195	26.012993167726396
24	25.068372777267655	24.680351095445435	24.74748970705844	25.50378642022847
25	25.456879103519768	24.715407019795627	24.508071284000593	25.319642592684012
26	25.706923107302238	24.316772639311242	24.576438529283994	25.399865724102522
27	25.512168633065112	24.899047242928372	24.26815421829491	25.320629905711606
28	25.65879762645261	24.234076794723695	24.289367416052052	25.817758162771643
29	25.685202006239884	24.658386319655623	24.52509774495478	25.13131392914972
30	26.011018522175274	24.3592275186604	24.018601161091585	25.611152798072744
31	24.9997531717431	24.970133780915237	24.513995162166164	25.516117885175493
32	25.643734449666283	24.598159630346352	24.025512420520517	25.732593499466844
33	25.230784420200425	24.610751838870513	24.33232956508861	25.826134175840448
34	25.95053561731747	24.529792170607692	24.22372513205312	25.29594708002172
35	26.053216172187394	24.40144147702029	24.14375277681789	25.40158957397443
36	25.571407414720838	24.41625117243422	24.559411561435553	25.45292985140939
37	26.01668559016636	24.52584291849731	24.01935133534087	25.43812015599546
38	25.90413190502049	24.14868934195587	24.25531914893617	25.691859604087476
39	25.384068559693535	24.75613127443624	24.509300580545794	25.35049958532443
40	26.343486202300436	24.12499382929358	23.87125438120156	25.66026558720442
41	25.17352026459989	24.368860147109643	24.19114380214247	26.266475786147996
42	25.537376948824537	24.27354140542462	24.588512919756315	25.60056872599453
43	25.51193696806935	24.417961730613534	24.387354120179303	25.68274718113781
44	25.66624208852948	24.2996929213938	24.156521223969904	25.877543766106815
45	25.455155799533983	24.366138778089333	24.286165633268826	25.892539789107854
46	25.721986907970734	24.340708707285525	24.27357009567252	25.663734289071215
47	25.677049908673546	24.446857876289677	24.13289233351434	25.74319988152244
48	25.76272190517565	24.69244288224956	23.939100728658598	25.605734483916194
49	25.66325371984874	24.74304163663471	23.892931546884412	25.70077309663214
50	25.44824454010505	24.12227005252557	24.099561628687653	26.329923778681724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	6.0
6	8.0
7	6.5
8	5.0
9	7.5
10	10.0
11	14.5
12	19.0
13	38.5
14	58.0
15	77.0
16	96.0
17	101.0
18	106.0
19	103.0
20	100.0
21	128.0
22	156.0
23	241.5
24	327.0
25	422.5
26	518.0
27	579.0
28	640.0
29	741.5
30	843.0
31	1017.5
32	1192.0
33	1497.5
34	1803.0
35	1935.5
36	2068.0
37	2355.5
38	2643.0
39	3174.0
40	3705.0
41	3977.5
42	4250.0
43	5075.5
44	5901.0
45	6199.5
46	6498.0
47	7126.0
48	7754.0
49	7893.5
50	8033.0
51	8421.5
52	8810.0
53	8770.5
54	8731.0
55	8265.5
56	7800.0
57	7555.0
58	7310.0
59	6810.0
60	6310.0
61	5755.5
62	5201.0
63	4521.0
64	3841.0
65	3140.0
66	2439.0
67	2003.0
68	1567.0
69	1322.5
70	1078.0
71	800.5
72	523.0
73	430.0
74	337.0
75	291.5
76	246.0
77	184.0
78	122.0
79	100.0
80	78.0
81	71.5
82	65.0
83	54.0
84	43.0
85	39.0
86	35.0
87	21.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02073357357950338
2	0.007898504220763193
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0019746260551907983
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	9.873130275953991E-4
24	0.0019746260551907983
25	0.0
26	9.873130275953991E-4
27	0.0
28	0.0019746260551907983
29	9.873130275953991E-4
30	9.873130275953991E-4
31	0.0
32	9.873130275953991E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	9.873130275953991E-4
40	0.0
41	0.0
42	0.005923878165572395
43	0.0029619390827861974
44	0.007898504220763193
45	9.873130275953991E-4
46	0.0019746260551907983
47	0.0
48	0.0029619390827861974
49	0.0039492521103815965
50	9.873130275953991E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	101285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.70193019696895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.52801882629798	35.189810929555215
2	16.252390057361378	17.455694327886658
3	7.559935284600676	12.179493508416844
4	3.932563612295926	8.447450264106235
5	2.2760700102956317	6.11146764081552
6	1.4211648771878218	4.579157821987461
7	0.9045447859979409	3.4003060670385548
8	0.5791292837181938	2.488028829540406
9	0.43388733637299604	2.097052870612628
>10	1.1086189145462568	7.944907933060177
>50	0.0036770113251948814	0.1066298069803031
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	9.873130275953991E-4	0.0
25	0.0	0.0	0.0	9.873130275953991E-4	0.0
26	0.0	0.0	0.0	9.873130275953991E-4	0.0
27	0.0	0.0	0.0	9.873130275953991E-4	0.0
28	0.0	0.0	0.0	9.873130275953991E-4	0.0
29	0.0	0.0	0.0	9.873130275953991E-4	0.0
30	0.0	0.0	0.0	0.0019746260551907983	0.0
31	0.0	0.0	0.0	0.005923878165572395	0.0
32	0.0	0.0	0.0	0.005923878165572395	0.0
33	0.0	0.0	0.0	0.007898504220763193	0.0
34	0.0	0.0	0.0	0.008885817248358591	0.0
35	0.0	0.0	0.0	0.012835069358740188	0.0
36	0.0	0.0	0.0	0.017771634496717183	0.0
37	0.0	0.0	0.0	0.02270819963469418	0.0
38	0.0	0.0	0.0	0.03455595596583897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCAA	25	0.0023426584	35.2	11
CATAATC	25	0.0023426584	35.2	2
TTAGGCT	30	0.0057205046	29.333336	4
GCGCGGG	30	0.0057205046	29.333336	13
ATAATCC	30	0.0057205046	29.333336	3
TATAACA	30	0.0057205046	29.333336	4
ATCAACG	85	2.3087523E-7	23.294119	2
TCAACGC	90	4.0059967E-7	21.999998	3
GTCCAAG	55	0.0044557876	20.0	1
GTATCAA	80	8.8854E-5	19.25	1
AACGCAG	110	2.745459E-6	18.0	5
ACGCAGA	135	1.0615968E-6	16.296295	6
TATCAAC	125	9.270594E-6	15.839998	1
CAACGCA	125	9.270594E-6	15.839998	4
ATGGGGG	85	0.0029547955	15.529412	17
CGCAGAG	145	2.2356799E-6	15.172415	7
GAGTACA	160	6.2058534E-6	13.75	11
AGAGTAC	145	3.768203E-5	13.655173	10
GCAGAGT	145	3.768203E-5	13.655173	8
AGTACAT	135	3.1622127E-4	13.037037	12
>>END_MODULE