FastQCFastQC Report
Thu 2 Feb 2017
SRR4061972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879696
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29370.15624866999770176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.013.7413781
GACCCGT500.001497643713.3024816
GTATCAA17950.013.1190581
TTATACC1103.81242E-812.0915544
TCGAACC802.8686689E-511.87436715
GTCCTAA1302.6357156E-911.6867041
GTGTAAC1850.011.29188251
GAACCGT600.005868276611.0854016
CTAAATC600.00587642911.08333413
GTCTTAT1900.010.9947271
TCTTATA1658.0035534E-1110.9367712
ACCGGTC700.001489001810.8594588
CCTTATG1053.4768327E-610.8545412
GTATTAA2100.010.8519391
GATCGTC909.4960305E-510.5578067
CTTATAC1451.44355E-810.48275853
GTAGGAC1201.5238584E-610.2916673
GTATAAC750.002658561310.1284761
ACCCGTG951.6407031E-410.0021317
CCGACCA1151.0235821E-59.9154219
Produced by FastQC (version 0.11.5)