##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061972_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1879696
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25315050944408	32.0	32.0	32.0	32.0	32.0
2	30.936529098322282	32.0	32.0	32.0	32.0	32.0
3	30.938138401103156	32.0	32.0	32.0	32.0	32.0
4	30.91030890101378	32.0	32.0	32.0	32.0	32.0
5	30.915685834305123	32.0	32.0	32.0	32.0	32.0
6	34.35302038201922	36.0	36.0	36.0	32.0	36.0
7	34.342272367446654	36.0	36.0	36.0	32.0	36.0
8	34.31695444369728	36.0	36.0	36.0	32.0	36.0
9	34.439556715554005	36.0	36.0	36.0	32.0	36.0
10	34.23665794894494	36.0	36.0	36.0	32.0	36.0
11	34.402041074726974	36.0	36.0	36.0	32.0	36.0
12	34.295923915356525	36.0	36.0	36.0	32.0	36.0
13	34.34295492462611	36.0	36.0	36.0	32.0	36.0
14	34.28127367404091	36.0	36.0	36.0	32.0	36.0
15	34.233421787352846	36.0	36.0	36.0	32.0	36.0
16	34.23666380095505	36.0	36.0	36.0	32.0	36.0
17	34.19443037597569	36.0	36.0	36.0	32.0	36.0
18	34.17200813323005	36.0	36.0	36.0	32.0	36.0
19	34.1645244762983	36.0	36.0	36.0	32.0	36.0
20	34.14506015866395	36.0	36.0	36.0	32.0	36.0
21	34.12837075782467	36.0	36.0	36.0	32.0	36.0
22	34.0859910325925	36.0	36.0	36.0	32.0	36.0
23	34.06528821681804	36.0	36.0	36.0	32.0	36.0
24	34.03539348915995	36.0	36.0	36.0	32.0	36.0
25	33.51687985716839	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	27.0
5	146.0
6	384.0
7	73.0
8	342.0
9	242.0
10	124.0
11	42.0
12	65.0
13	82.0
14	386.0
15	743.0
16	1218.0
17	1508.0
18	1923.0
19	2381.0
20	2992.0
21	3748.0
22	4692.0
23	6402.0
24	8339.0
25	11489.0
26	15753.0
27	21180.0
28	29501.0
29	41449.0
30	58383.0
31	88275.0
32	139288.0
33	222417.0
34	503439.0
35	712662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39335418794688	17.301455566905005	12.27964334354784	26.025546901600272
2	16.466474294724883	19.30928045658544	37.465765667992414	26.758479580697266
3	18.97018970189702	22.538338850212217	28.340903746689605	30.150567701201158
4	13.403299308055134	15.86455864239406	34.69657911608765	36.035562933463154
5	15.282141671136994	35.60952946585945	33.58665202101481	15.521676841988743
6	34.65610747000882	34.21534245525277	16.80416618757426	14.324383887164155
7	30.610410344948786	30.28002125907851	20.022515877741125	19.087052518231577
8	28.121338769528336	33.08165369392859	19.02833339723845	19.768674139304625
9	27.118124108899	15.14433639239175	18.55306235163851	39.184477147070744
10	16.47764711271766	27.10487668851165	31.334631993816714	25.08284420495398
11	36.03041764329853	21.68804602599616	22.28084380992968	20.000692520775623
12	24.39012957266323	23.68977135019243	28.53272608631963	23.387372990824712
13	29.176485126365176	20.14822500713747	24.792267670031578	25.88302219646578
14	23.40568537381	19.244258038745755	25.40635110844817	31.94370547899607
15	24.981556282236557	26.753804466229486	22.295884640604516	25.96875461092944
16	25.296312589943	26.127063695889063	23.503630741306086	25.072992972861847
17	24.14975914369779	26.009284845334246	25.050866379613396	24.79008963135457
18	24.283154169584677	26.01175370998107	25.469176468082043	24.235915652352208
19	25.240262243683	25.271786013244242	25.257834615228962	24.230117127843794
20	25.550230826983594	25.008652683929437	24.907749077490777	24.533367411596192
21	25.593479986773392	24.756646981176583	25.02133881566511	24.62853421638491
22	25.624029800275316	24.682999305050764	24.84025486797296	24.85271602670096
23	24.94658429671498	24.90079560513749	24.99226650296322	25.160353595184308
24	24.462723577863482	25.144126553417212	25.125223837443034	25.26792603127627
25	24.67364224150554	24.835829414874897	25.25977369404982	25.230754649569747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	111.0
2	304.5
3	498.0
4	498.0
5	498.0
6	1023.0
7	1548.0
8	1548.0
9	1548.0
10	2025.0
11	2502.0
12	2502.0
13	2502.0
14	3559.0
15	4616.0
16	4616.0
17	4616.0
18	8264.0
19	11912.0
20	11912.0
21	11912.0
22	17736.5
23	23561.0
24	23561.0
25	23561.0
26	35197.5
27	46834.0
28	46834.0
29	46834.0
30	61138.5
31	75443.0
32	75443.0
33	75443.0
34	93333.0
35	111223.0
36	111223.0
37	111223.0
38	133465.0
39	155707.0
40	155707.0
41	155707.0
42	177713.5
43	199720.0
44	199720.0
45	199720.0
46	222621.5
47	245523.0
48	245523.0
49	245523.0
50	255158.5
51	264794.0
52	264794.0
53	264794.0
54	252965.5
55	241137.0
56	241137.0
57	241137.0
58	219771.5
59	198406.0
60	198406.0
61	198406.0
62	169697.0
63	140988.0
64	140988.0
65	140988.0
66	112695.0
67	84402.0
68	84402.0
69	84402.0
70	62570.0
71	40738.0
72	40738.0
73	40738.0
74	29482.0
75	18226.0
76	18226.0
77	18226.0
78	12907.0
79	7588.0
80	7588.0
81	7588.0
82	4993.0
83	2398.0
84	2398.0
85	2398.0
86	1659.5
87	921.0
88	921.0
89	921.0
90	579.0
91	237.0
92	237.0
93	237.0
94	174.5
95	112.0
96	112.0
97	112.0
98	331.5
99	551.0
100	551.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.512014708761416E-4
2	2.128003677190354E-4
3	5.320009192975885E-4
4	0.011225219397179118
5	0.034048058835045664
6	0.06086090516764413
7	0.10161217558583942
8	0.10171857576969892
9	0.11187979332828288
10	0.1264566185170368
11	0.1327874294566781
12	0.12613741796545824
13	0.12129620959985019
14	0.11017739038653059
15	0.12571181723002017
16	0.11267779470722926
17	0.11852980481950272
18	0.10592138303214989
19	0.09299376069321848
20	0.08863135315497825
21	0.08847175287918897
22	0.09900537108128123
23	0.07995973817042756
24	0.08825895251146994
25	0.08613094883427959
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1879696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.32996579402119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.478126160389365	20.307002311509905
2	15.447957314425913	10.915516070123338
3	8.190814727327187	8.681436124249036
4	4.9484453493640235	6.993136197064567
5	3.241354663750304	5.725847469829464
6	2.2982196732061975	4.871761346491291
7	1.6677752845666156	4.1245710629096735
8	1.2365688004757562	3.4950346738209825
9	0.9412978717089207	2.9930419448515
>10	4.37166109829662	25.60772660507923
>50	0.13340542661257299	3.170048173144171
>100	0.042864590551990225	2.5711787108112967
>500	0.0010563275271957768	0.2280786084207195
>1k	4.527117973696186E-4	0.3156207016948713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2937	0.15624866999770176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.3200091929758855E-5
2	0.0	0.0	0.0	0.0	5.3200091929758855E-5
3	0.0	0.0	0.0	0.0	5.3200091929758855E-5
4	0.0	0.0	0.0	0.0	5.3200091929758855E-5
5	0.0	0.0	0.0	0.0	5.3200091929758855E-5
6	0.0	0.0	0.0	0.0	3.1920055157855313E-4
7	0.0	0.0	0.0	0.0	3.7240064350831196E-4
8	0.0	0.0	0.0	0.0	4.2560073543807084E-4
9	0.0	0.0	0.0	0.0	5.852010112273474E-4
10	0.0	0.0	0.0	0.0	5.852010112273474E-4
11	0.0	0.0	0.0	0.0	5.852010112273474E-4
12	0.0	0.0	0.0	0.0	5.852010112273474E-4
13	0.0	0.0	0.0	0.0	5.852010112273474E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	615	0.0	13.741378	1
GACCCGT	50	0.0014976437	13.302481	6
GTATCAA	1795	0.0	13.119058	1
TTATACC	110	3.81242E-8	12.091554	4
TCGAACC	80	2.8686689E-5	11.874367	15
GTCCTAA	130	2.6357156E-9	11.686704	1
GTGTAAC	185	0.0	11.2918825	1
GAACCGT	60	0.0058682766	11.085401	6
CTAAATC	60	0.005876429	11.083334	13
GTCTTAT	190	0.0	10.994727	1
TCTTATA	165	8.0035534E-11	10.936771	2
ACCGGTC	70	0.0014890018	10.859458	8
CCTTATG	105	3.4768327E-6	10.854541	2
GTATTAA	210	0.0	10.851939	1
GATCGTC	90	9.4960305E-5	10.557806	7
CTTATAC	145	1.44355E-8	10.4827585	3
GTAGGAC	120	1.5238584E-6	10.291667	3
GTATAAC	75	0.0026585613	10.128476	1
ACCCGTG	95	1.6407031E-4	10.002131	7
CCGACCA	115	1.0235821E-5	9.915421	9
>>END_MODULE