FastQCFastQC Report
Thu 2 Feb 2017
SRR4061972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879696
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18100.027.8456171
CGGATCG1107.2759576E-1226.00023726
GGTATCA9750.023.701981
CGCAATA602.8734226E-422.00019836
GGACGTA708.126571E-418.85681319
GCGTACT708.1280246E-418.85631214
TAGTACG600.00741548118.3325254
ATCGATT1456.313712E-918.20657729
ATCAACG27500.018.1591993
TATCAAC27600.018.0948472
TCAACGC28000.017.7563614
CAACGCA27950.017.7094155
AACGCAG28350.017.5371466
TCGGATC2101.5097612E-1015.71484725
GGCGTTA1704.4921762E-815.52996542
TACTATA2455.456968E-1215.265852
TAACGCT900.00436148214.6660194
TCTAACC2254.274625E-1014.6660193
CCTATAT1851.266144E-714.270782
ACCGTCG1707.600611E-714.2346658
Produced by FastQC (version 0.11.5)