##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1879696
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18458303895949	32.0	32.0	32.0	32.0	32.0
2	31.183597773256952	32.0	32.0	32.0	32.0	32.0
3	31.412499148798528	32.0	32.0	32.0	32.0	32.0
4	31.538767970991053	32.0	32.0	32.0	32.0	32.0
5	31.4638579855466	32.0	32.0	32.0	32.0	32.0
6	35.10311561018377	36.0	36.0	36.0	36.0	36.0
7	35.11647468526826	36.0	36.0	36.0	36.0	36.0
8	35.05365974072403	36.0	36.0	36.0	36.0	36.0
9	35.18148572960734	36.0	36.0	36.0	36.0	36.0
10	34.999615363335344	36.0	36.0	36.0	36.0	36.0
11	35.18827618934125	36.0	36.0	36.0	36.0	36.0
12	35.092201079323466	36.0	36.0	36.0	36.0	36.0
13	35.13694395263915	36.0	36.0	36.0	36.0	36.0
14	35.097270516083455	36.0	36.0	36.0	36.0	36.0
15	35.054451358091946	36.0	36.0	36.0	36.0	36.0
16	35.076926268928595	36.0	36.0	36.0	36.0	36.0
17	35.06027730015917	36.0	36.0	36.0	36.0	36.0
18	35.05841636094347	36.0	36.0	36.0	36.0	36.0
19	35.04563291085367	36.0	36.0	36.0	36.0	36.0
20	35.05250902273559	36.0	36.0	36.0	36.0	36.0
21	35.03773056919842	36.0	36.0	36.0	36.0	36.0
22	35.02163860539151	36.0	36.0	36.0	36.0	36.0
23	34.98536146270461	36.0	36.0	36.0	36.0	36.0
24	34.97201515564219	36.0	36.0	36.0	32.0	36.0
25	34.958771524757196	36.0	36.0	36.0	32.0	36.0
26	34.900800448583176	36.0	36.0	36.0	32.0	36.0
27	34.88096958231544	36.0	36.0	36.0	32.0	36.0
28	34.86999387134941	36.0	36.0	36.0	32.0	36.0
29	34.853070390105636	36.0	36.0	36.0	32.0	36.0
30	34.83240534639644	36.0	36.0	36.0	32.0	36.0
31	34.8214046313872	36.0	36.0	36.0	32.0	36.0
32	34.79109547501298	36.0	36.0	36.0	32.0	36.0
33	34.769909070402875	36.0	36.0	36.0	32.0	36.0
34	34.75838114248261	36.0	36.0	36.0	32.0	36.0
35	34.756042998442304	36.0	36.0	36.0	32.0	36.0
36	34.7173840876397	36.0	36.0	36.0	32.0	36.0
37	34.7115698495927	36.0	36.0	36.0	32.0	36.0
38	34.70056541057703	36.0	36.0	36.0	32.0	36.0
39	34.6799243069092	36.0	36.0	36.0	32.0	36.0
40	34.670038665826816	36.0	36.0	36.0	32.0	36.0
41	34.64241824209872	36.0	36.0	36.0	32.0	36.0
42	34.61131959636026	36.0	36.0	36.0	32.0	36.0
43	34.59099822524493	36.0	36.0	36.0	32.0	36.0
44	34.55900102995378	36.0	36.0	36.0	32.0	36.0
45	34.54734169780645	36.0	36.0	36.0	32.0	36.0
46	34.52597122087827	36.0	36.0	36.0	32.0	36.0
47	34.50294515708923	36.0	36.0	36.0	32.0	36.0
48	34.47199653561001	36.0	36.0	36.0	32.0	36.0
49	34.45311795098782	36.0	36.0	36.0	32.0	36.0
50	33.9763876711979	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	7.0
21	29.0
22	112.0
23	258.0
24	780.0
25	1915.0
26	4245.0
27	8437.0
28	14914.0
29	24732.0
30	38676.0
31	60506.0
32	96504.0
33	170906.0
34	384858.0
35	1072812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76698835380796	17.918160024053364	12.843166210344542	26.471685411794134
2	16.04059120491972	19.919568806231474	36.82639939264287	27.213440596205935
3	18.482167034723222	23.039826184382235	28.622173940106176	29.855832840788366
4	13.254643304023631	16.373232692946093	34.69177994739575	35.68034405563453
5	15.072658064207225	36.13314926091733	33.05802271113133	15.736169963744118
6	34.96065580921991	34.37082544862379	16.584729527640953	14.083789214515349
7	30.81354644580826	30.249944671904395	19.889386368859647	19.047122513427702
8	28.297926898817682	33.15865969816396	18.791123671061705	19.752289731956658
9	27.246427081825995	14.916454575633505	18.334720082396302	39.5023982601442
10	16.386000715009235	27.142208101735598	31.354963781377414	25.11682740187775
11	36.343696001906686	21.546622432563563	22.177628722942433	19.93205284258731
12	24.58073007550157	23.610679599254347	28.427203122206997	23.38138720303709
13	29.49780177220146	19.95891888901184	24.816832083485842	25.726447255300855
14	23.570087929111942	19.038557298627012	25.248125228760394	32.14322954350065
15	25.182795515870655	26.694369727870892	22.192152348039258	25.9306824082192
16	25.57181184274157	25.924158488425974	23.489279841334948	25.014749827497507
17	24.339680458967834	25.75937811220538	25.04357087529047	24.857370553536317
18	24.58354879416415	25.87248336429585	25.334576090125406	24.209391751414593
19	25.532798920676537	25.19668073986432	25.167367489211024	24.103152850248126
20	25.837357483376273	24.869805867234703	24.766118722578632	24.526717926810388
21	25.866044296524542	24.632653365225014	24.860190158408592	24.641112179841848
22	25.82151581957934	24.690694665520383	24.77347400856309	24.714315506337194
23	25.167686689762597	24.752566372434693	24.904080234250646	25.175666703552064
24	24.713887470207695	25.115072778345247	25.005266853932582	25.165772897514472
25	25.04403644637715	24.610667805859787	25.168524860176632	25.17677088758643
26	24.91678126920202	24.874859351433884	25.211085900031122	24.997273479332975
27	25.204700980426058	24.93274167643333	24.868156661752742	24.994400681387866
28	25.19897130769902	24.71771610731257	25.166146362017123	24.91716622297129
29	24.978294316302886	25.082407117761846	24.864392677098163	25.074905888837108
30	25.220463057541707	24.875617126319373	24.91243190330269	24.991487912836227
31	25.116654936002714	24.780003500579085	24.975780567955656	25.127560995462545
32	25.160664961419126	25.022663335617402	24.74575567860198	25.070916024361487
33	25.17778169094634	24.88821312842044	24.83235294274118	25.10165223789204
34	25.17842085243006	25.023075674913613	25.057070732596152	24.74143274006017
35	25.323416830922035	24.79306483232652	25.090506704545152	24.793011632206287
36	25.14008921660163	24.802534453727866	25.021931749565756	25.03544458010475
37	25.388245721463253	24.723874844188323	24.890446370193114	24.99743306415531
38	25.305794128412252	24.863488564108238	24.928233075986757	24.902484231492753
39	25.30201884997691	25.07134177872451	24.688830675794442	24.93780869550414
40	25.463904801627496	24.855348950042984	24.75517317693925	24.925573071390268
41	25.166330972300692	24.871228492418414	24.86463164592384	25.097808889357054
42	25.32315042402188	24.913014332684266	24.88550877304987	24.878326470243987
43	25.298901752524834	24.465333887342783	24.95638771652521	25.279376643607176
44	24.981924643719964	24.941225166003328	25.14482895844982	24.932021231826884
45	25.191031787114802	24.988442064819136	24.787927184742355	25.032598963323704
46	25.112892757200772	24.95025696136535	24.737824925784448	25.199025355649425
47	25.141646902601195	24.987950038144913	24.817601350021334	25.052801709232558
48	25.274694873621474	25.167014163950263	24.466504329839207	25.09178663258906
49	25.152277228881626	24.864933596009003	24.75879574235581	25.223993432753556
50	25.109021738378683	25.058959786047662	24.785294873411356	25.04672360216229
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	34.0
2	37.0
3	99.0
4	161.0
5	178.0
6	195.0
7	166.0
8	137.0
9	207.0
10	277.0
11	451.0
12	625.0
13	1128.0
14	1631.0
15	2079.0
16	2527.0
17	2535.5
18	2544.0
19	2492.5
20	2441.0
21	3018.5
22	3596.0
23	5072.5
24	6549.0
25	8048.0
26	9547.0
27	12208.5
28	14870.0
29	18151.5
30	21433.0
31	25480.5
32	29528.0
33	34164.5
34	38801.0
35	44788.5
36	50776.0
37	57526.0
38	64276.0
39	72384.0
40	80492.0
41	86986.5
42	93481.0
43	100123.5
44	106766.0
45	114698.5
46	122631.0
47	128521.5
48	134412.0
49	138612.0
50	142812.0
51	147455.0
52	152098.0
53	154179.5
54	156261.0
55	152140.5
56	148020.0
57	139693.0
58	131366.0
59	121362.0
60	111358.0
61	97671.0
62	83984.0
63	72131.0
64	60278.0
65	50150.5
66	40023.0
67	33077.5
68	26132.0
69	21004.5
70	15877.0
71	12490.0
72	9103.0
73	7383.5
74	5664.0
75	4582.5
76	3501.0
77	2877.5
78	2254.0
79	1858.5
80	1463.0
81	1170.5
82	878.0
83	674.5
84	471.0
85	345.5
86	220.0
87	142.5
88	65.0
89	45.0
90	25.0
91	24.5
92	24.0
93	23.0
94	22.0
95	18.5
96	15.0
97	12.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02878124973399954
2	0.004096407078591432
3	1.5960027578927656E-4
4	0.0
5	5.320009192975885E-5
6	1.5960027578927656E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.916011950868651E-4
17	0.0
18	2.128003677190354E-4
19	0.0
20	1.5960027578927656E-4
21	0.0
22	0.0
23	0.0
24	8.512014708761416E-4
25	1.5960027578927656E-4
26	5.852010112273474E-4
27	1.5960027578927656E-4
28	0.0014896025740332478
29	2.128003677190354E-4
30	8.512014708761416E-4
31	3.7240064350831196E-4
32	4.256007354380708E-4
33	1.5960027578927656E-4
34	5.852010112273474E-4
35	5.320009192975885E-5
36	5.320009192975885E-5
37	0.0012236021143844536
38	0.0
39	6.384011031571063E-4
40	0.0
41	5.320009192975885E-4
42	0.004043206986661673
43	0.0034580059754343254
44	0.0034580059754343254
45	0.00186200321754156
46	0.0019152033094713187
47	0.0011704020224546949
48	0.0034580059754343254
49	0.0034580059754343254
50	0.0013300022982439715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1879696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.461626448902635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.71640944421603	21.04434161126921
2	15.612104049789444	11.384854118896454
3	8.119597240250624	8.88161164468677
4	4.914735592715505	7.167970131068753
5	3.2451115434387154	5.9161022440942155
6	2.181351439785583	4.772137281074271
7	1.6316085363389192	4.1643770673981315
8	1.2265629540688632	3.5777984197857124
9	0.9115258860855329	2.991214472126011
>10	4.302685073259335	25.72159807805247
>50	0.10927302742707404	2.663021675208159
>100	0.028595389440989133	1.608650143392384
>500	4.3982318347751383E-4	0.10632311294744594
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0640018385951771E-4
2	0.0	0.0	0.0	0.0	1.0640018385951771E-4
3	0.0	0.0	0.0	0.0	1.0640018385951771E-4
4	0.0	0.0	0.0	0.0	2.1280036771903542E-4
5	0.0	0.0	0.0	0.0	2.1280036771903542E-4
6	0.0	0.0	0.0	0.0	2.6600045964879425E-4
7	0.0	0.0	0.0	0.0	2.6600045964879425E-4
8	0.0	0.0	0.0	0.0	2.6600045964879425E-4
9	0.0	0.0	0.0	0.0	3.7240064350831196E-4
10	0.0	0.0	0.0	0.0	3.7240064350831196E-4
11	0.0	0.0	0.0	0.0	3.7240064350831196E-4
12	0.0	0.0	0.0	0.0	3.7240064350831196E-4
13	0.0	0.0	0.0	0.0	5.320009192975885E-4
14	0.0	0.0	0.0	0.0	5.320009192975885E-4
15	0.0	0.0	0.0	0.0	9.044015628059005E-4
16	0.0	0.0	0.0	5.3200091929758855E-5	9.044015628059005E-4
17	0.0	0.0	0.0	1.5960027578927656E-4	9.044015628059005E-4
18	0.0	0.0	0.0	3.1920055157855313E-4	0.0010108017466654183
19	0.0	0.0	0.0	5.320009192975885E-4	0.0011704020224546949
20	0.0	0.0	0.0	6.916011950868651E-4	0.0011704020224546949
21	0.0	0.0	0.0	9.044015628059005E-4	0.0011704020224546949
22	0.0	0.0	0.0	0.0011704020224546949	0.0011704020224546949
23	0.0	0.0	0.0	0.0016492028498225244	0.0015428026659630068
24	0.0	0.0	0.0	0.001968403401401078	0.0015428026659630068
25	0.0	0.0	0.0	0.002926005056136737	0.0015428026659630068
26	0.0	0.0	0.0	0.00372400643508312	0.0015428026659630068
27	0.0	0.0	0.0	0.0046816080898187794	0.0016492028498225244
28	0.0	0.0	0.0	0.005586009652624679	0.0016492028498225244
29	0.0	0.0	0.0	0.007235212502447204	0.0016492028498225244
30	0.0	0.0	0.0	0.009469616363497076	0.0016492028498225244
31	0.0	0.0	0.0	0.013406423166299232	0.0016492028498225244
32	0.0	0.0	0.0	0.019311633370502464	0.001808803125611801
33	0.0	0.0	0.0	0.02505724329891642	0.00186200321754156
34	0.0	0.0	0.0	0.03160085460627676	0.0020216034933308365
35	0.0	0.0	0.0	0.03841046637328589	0.0020216034933308365
36	0.0	0.0	0.0	0.048997284667307905	0.0020748035852605953
37	0.0	0.0	0.0	0.06442531132693798	0.002128003677190354
38	0.0	0.0	0.0	0.08368374460551067	0.002128003677190354
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1810	0.0	27.845617	1
CGGATCG	110	7.2759576E-12	26.000237	26
GGTATCA	975	0.0	23.70198	1
CGCAATA	60	2.8734226E-4	22.000198	36
GGACGTA	70	8.126571E-4	18.856813	19
GCGTACT	70	8.1280246E-4	18.856312	14
TAGTACG	60	0.007415481	18.332525	4
ATCGATT	145	6.313712E-9	18.206577	29
ATCAACG	2750	0.0	18.159199	3
TATCAAC	2760	0.0	18.094847	2
TCAACGC	2800	0.0	17.756361	4
CAACGCA	2795	0.0	17.709415	5
AACGCAG	2835	0.0	17.537146	6
TCGGATC	210	1.5097612E-10	15.714847	25
GGCGTTA	170	4.4921762E-8	15.529965	42
TACTATA	245	5.456968E-12	15.26585	2
TAACGCT	90	0.004361482	14.666019	4
TCTAACC	225	4.274625E-10	14.666019	3
CCTATAT	185	1.266144E-7	14.27078	2
ACCGTCG	170	7.600611E-7	14.234665	8
>>END_MODULE