FastQCFastQC Report
Thu 2 Feb 2017
SRR4061971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5612948
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90760.16169756071141225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.013.76991751
GTATCAA61300.012.4089611
GCGTAAC4550.011.06737911
CGTCGTA7650.011.05363310
CGCGTAA4400.011.01270310
TCGCGTA4400.011.0126049
CGGTCCA7950.010.995048510
CCGTCGT7250.010.7460389
CGACCAT8450.010.68177310
AACCGCG2650.010.3972127
AAGACGG10150.010.202125
GTCTTAG6250.010.1802431
CGCCGGT8400.010.1795377
CTAGACA3550.010.1687994
CGGACCA10100.010.1595619
CCGACCA8950.010.0849369
ACCGTCG8150.010.0254918
CGTTATT4550.010.019892
GCCGGTC9250.09.86039458
CAAGACG11000.09.8452464
Produced by FastQC (version 0.11.5)