##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061971_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5612948
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254481424021744	32.0	32.0	32.0	32.0	32.0
2	30.811478745215528	32.0	32.0	32.0	32.0	32.0
3	30.83173565833854	32.0	32.0	32.0	32.0	32.0
4	30.83724969481278	32.0	32.0	32.0	32.0	32.0
5	30.767620687025783	32.0	32.0	32.0	32.0	32.0
6	34.41612607136214	36.0	36.0	36.0	32.0	36.0
7	34.3476864563862	36.0	36.0	36.0	32.0	36.0
8	34.31818146186282	36.0	36.0	36.0	32.0	36.0
9	34.44095901120053	36.0	36.0	36.0	32.0	36.0
10	34.145963939092255	36.0	36.0	36.0	32.0	36.0
11	34.42088114837337	36.0	36.0	36.0	32.0	36.0
12	34.25198897263969	36.0	36.0	36.0	32.0	36.0
13	34.328842348085175	36.0	36.0	36.0	32.0	36.0
14	34.24626666771187	36.0	36.0	36.0	32.0	36.0
15	34.19378266109004	36.0	36.0	36.0	32.0	36.0
16	34.20020744891989	36.0	36.0	36.0	32.0	36.0
17	34.14220495183636	36.0	36.0	36.0	32.0	36.0
18	34.14392615075002	36.0	36.0	36.0	32.0	36.0
19	34.14827413330749	36.0	36.0	36.0	32.0	36.0
20	34.12943412267493	36.0	36.0	36.0	32.0	36.0
21	34.11416104335903	36.0	36.0	36.0	32.0	36.0
22	34.085495001913436	36.0	36.0	36.0	32.0	36.0
23	34.05880688721862	36.0	36.0	36.0	32.0	36.0
24	34.03123599220944	36.0	36.0	36.0	32.0	36.0
25	33.686838360162966	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	83.0
5	453.0
6	1205.0
7	294.0
8	958.0
9	767.0
10	418.0
11	113.0
12	211.0
13	254.0
14	721.0
15	1039.0
16	1619.0
17	2377.0
18	3362.0
19	4593.0
20	7223.0
21	10667.0
22	16273.0
23	23541.0
24	33826.0
25	48024.0
26	64847.0
27	83431.0
28	110068.0
29	145311.0
30	189853.0
31	258108.0
32	364187.0
33	518518.0
34	1148037.0
35	2572560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92216555686702	17.16010066142574	12.182027926864574	25.73570585484266
2	16.850129771656047	19.254343894175882	37.19703912533476	26.69848720883331
3	19.25226295313179	22.805251252069112	27.894875901248355	30.047609893550742
4	13.291860524785331	15.426936870673828	34.73479567924464	36.5464069252962
5	15.52280403343217	35.696719463525504	32.87879190226774	15.901684600774585
6	35.15219453860298	34.27043210477393	16.439117371153152	14.138255985469932
7	31.063755637337	29.882853256769355	19.90568975535839	19.14770135053526
8	28.452917913457203	32.43179757751801	19.01529394411608	20.0999905649087
9	26.901580770259166	15.03471786815683	18.5330646066108	39.53063675497321
10	16.55376933764442	26.431123055174773	30.35961114556209	26.655496461618718
11	36.90378954858052	21.49592270535538	21.857282376873837	19.74300536919026
12	25.00181075973056	23.311595891234532	28.0564999851928	23.630093363842107
13	29.03286344775422	19.658325038086776	25.07285545577474	26.235956058384264
14	23.842554730034525	19.35060095881573	24.81191218936013	31.99493212178961
15	25.301505090290078	27.052359359172566	21.903072370855277	25.743063179682085
16	26.142493257416845	25.984773891576406	22.95712216561782	24.915610685388927
17	24.17642655927574	25.93998575795371	24.949696179192912	24.933891503577637
18	24.850368556109405	25.265253917296533	25.561796800882675	24.322580725711386
19	25.672367801103608	24.826881229912782	25.14588700475905	24.354863964224556
20	25.930151626498105	24.388760304875774	24.61883840695791	25.062249661668208
21	26.581214169904467	24.282274637303317	24.411061473155915	24.7254497196363
22	26.04967260765674	24.169123996751225	24.809070178636333	24.9721332169557
23	24.699870833193657	24.34163086822775	25.095002863637365	25.863495434941225
24	24.689904115786486	24.767458179385812	25.117173686685508	25.425464018142197
25	25.02298533289681	24.431732669700946	25.078941868684385	25.466340128717864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	390.0
1	390.0
2	989.0
3	1588.0
4	1588.0
5	1588.0
6	3098.0
7	4608.0
8	4608.0
9	4608.0
10	5382.0
11	6156.0
12	6156.0
13	6156.0
14	8454.0
15	10752.0
16	10752.0
17	10752.0
18	19246.0
19	27740.0
20	27740.0
21	27740.0
22	45940.0
23	64140.0
24	64140.0
25	64140.0
26	97054.0
27	129968.0
28	129968.0
29	129968.0
30	169185.5
31	208403.0
32	208403.0
33	208403.0
34	269914.5
35	331426.0
36	331426.0
37	331426.0
38	398183.5
39	464941.0
40	464941.0
41	464941.0
42	538181.5
43	611422.0
44	611422.0
45	611422.0
46	671245.0
47	731068.0
48	731068.0
49	731068.0
50	756054.5
51	781041.0
52	781041.0
53	781041.0
54	732671.0
55	684301.0
56	684301.0
57	684301.0
58	630605.5
59	576910.0
60	576910.0
61	576910.0
62	502426.5
63	427943.0
64	427943.0
65	427943.0
66	349392.0
67	270841.0
68	270841.0
69	270841.0
70	205011.5
71	139182.0
72	139182.0
73	139182.0
74	105010.0
75	70838.0
76	70838.0
77	70838.0
78	55843.5
79	40849.0
80	40849.0
81	40849.0
82	28384.5
83	15920.0
84	15920.0
85	15920.0
86	11753.5
87	7587.0
88	7587.0
89	7587.0
90	4959.0
91	2331.0
92	2331.0
93	2331.0
94	1432.0
95	533.0
96	533.0
97	533.0
98	1301.5
99	2070.0
100	2070.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013005643380270048
2	2.137913980318364E-4
3	6.948220436034683E-4
4	0.010618306102247874
5	0.03465202243099348
6	0.06438684270725473
7	0.10928303629394037
8	0.11079739202999922
9	0.12038237304175987
10	0.13468858076005694
11	0.14263449438690684
12	0.13479547645907283
13	0.1305552803981081
14	0.11860077805816123
15	0.13486674025841677
16	0.12093466748667545
17	0.12492544024993639
18	0.11293530601031758
19	0.09845093879366067
20	0.09419292678285991
21	0.09321304954188067
22	0.10536352733002335
23	0.08366370042979197
24	0.09348028878942047
25	0.0895073319759955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5612948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.443907627792505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.02092463507624	15.342999715290667
2	14.158204157843034	7.4879648585085805
3	7.551386577213992	5.990645073287971
4	4.618081770745728	4.884805110527699
5	3.195158378459008	4.224623650806865
6	2.2546284857042305	3.5772712446571417
7	1.631507728030186	3.02004077578419
8	1.2927347296619545	2.734796622273603
9	0.9874347943156215	2.3500471040415527
>10	5.7375012184025	27.162342849956893
>50	0.34589626416312896	6.23025951726274
>100	0.1803994601446477	8.80054537314165
>500	0.01540137580348002	2.817593222720867
>1k	0.010605324758045343	5.117732821029213
>5k	1.3509967844614563E-4	0.25833206071033893
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9076	0.16169756071141225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9597544819585001E-4
2	0.0	1.7815949835986364E-5	0.0	0.0	1.9597544819585001E-4
3	0.0	1.7815949835986364E-5	0.0	0.0	1.9597544819585001E-4
4	0.0	1.7815949835986364E-5	0.0	0.0	1.9597544819585001E-4
5	0.0	1.603435485238773E-4	0.0	0.0	2.137913980318364E-4
6	0.0	1.603435485238773E-4	0.0	0.0	3.0287114721176824E-4
7	0.0	1.603435485238773E-4	0.0	1.7815949835986364E-5	3.206870970477546E-4
8	0.0	1.603435485238773E-4	0.0	3.563189967197273E-5	3.206870970477546E-4
9	0.0	1.603435485238773E-4	0.0	3.563189967197273E-5	3.206870970477546E-4
10	0.0	1.603435485238773E-4	0.0	3.563189967197273E-5	3.206870970477546E-4
11	0.0	1.603435485238773E-4	0.0	3.563189967197273E-5	3.3850304688374096E-4
12	0.0	2.3160734786782276E-4	0.0	3.563189967197273E-5	8.907974917993183E-4
13	0.0	2.494232977038091E-4	0.0	3.563189967197273E-5	0.0011758526891751003
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2200	0.0	13.7699175	1
GTATCAA	6130	0.0	12.408961	1
GCGTAAC	455	0.0	11.067379	11
CGTCGTA	765	0.0	11.053633	10
CGCGTAA	440	0.0	11.012703	10
TCGCGTA	440	0.0	11.012604	9
CGGTCCA	795	0.0	10.9950485	10
CCGTCGT	725	0.0	10.746038	9
CGACCAT	845	0.0	10.681773	10
AACCGCG	265	0.0	10.397212	7
AAGACGG	1015	0.0	10.20212	5
GTCTTAG	625	0.0	10.180243	1
CGCCGGT	840	0.0	10.179537	7
CTAGACA	355	0.0	10.168799	4
CGGACCA	1010	0.0	10.159561	9
CCGACCA	895	0.0	10.084936	9
ACCGTCG	815	0.0	10.025491	8
CGTTATT	455	0.0	10.01989	2
GCCGGTC	925	0.0	9.8603945	8
CAAGACG	1100	0.0	9.845246	4
>>END_MODULE