FastQCFastQC Report
Thu 2 Feb 2017
SRR4061971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5612948
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66550.028.6352541
GGTATCA26250.023.3886991
TCAACGC101950.018.3199374
ATCAACG102950.018.1635173
CAACGCA105300.017.7580015
TATCAAC107000.017.7233722
AACGCAG107100.017.4800876
ATACGAA12350.016.38998440
CGAATGC12450.016.25862943
CGCAATA12150.016.11551936
TACGAAT12700.015.76518941
CTAGCGG12750.015.52900329
ATAGCGT7650.015.2411576
ACCGTCG13350.014.9955938
TAGCGGC13000.014.89191530
GTATAGT6100.014.790581
GGCGTTA15950.014.62199842
CGCGGTT19900.014.48190310
CGTCGTA13800.014.34732110
ACGCAGA131200.014.2691887
Produced by FastQC (version 0.11.5)