FastQCFastQC Report
Thu 2 Feb 2017
SRR4061970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118790
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33010.15579646873923325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.015.5282181
GCGTTAT1550.012.8656871
TCGCGTA1501.8189894E-1212.6683529
CGCGTAA1501.8189894E-1212.66805310
GTATCAA23500.012.4863641
CGCATCG2550.011.92259213
GTCTTAG2400.011.8701281
CGTCGTA2450.011.63392510
CCGTCGT2650.011.4732259
AACCCGG1501.7644197E-1011.40151711
GTCTACG600.00589482711.0787861
CGACCAT4150.010.98915510
TAGAACC1855.456968E-1210.78368954
AATCGAA1252.237648E-710.6411667
CGTTATT1701.4006218E-1010.6155472
GCGTAAC1803.45608E-1110.5569611
CCGACCA3700.010.5284289
TTAATAC1909.094947E-1210.4989163
CGAACGA1002.3997272E-510.44990916
TAGGACT2200.010.3635464
Produced by FastQC (version 0.11.5)