##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061970_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2118790
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236729926042695	32.0	32.0	32.0	32.0	32.0
2	30.714087285667762	32.0	32.0	32.0	32.0	32.0
3	30.743235525936974	32.0	32.0	32.0	32.0	32.0
4	30.756547369017223	32.0	32.0	32.0	32.0	32.0
5	30.639005753283715	32.0	32.0	32.0	32.0	32.0
6	34.317710580095245	36.0	36.0	36.0	32.0	36.0
7	34.256404834834974	36.0	36.0	36.0	32.0	36.0
8	34.196287975684236	36.0	36.0	36.0	32.0	36.0
9	34.35549865725249	36.0	36.0	36.0	32.0	36.0
10	33.993997517451	36.0	36.0	36.0	32.0	36.0
11	34.34901146409035	36.0	36.0	36.0	32.0	36.0
12	34.13572085954719	36.0	36.0	36.0	32.0	36.0
13	34.246471335054444	36.0	36.0	36.0	32.0	36.0
14	34.124706082245055	36.0	36.0	36.0	32.0	36.0
15	34.07113022055041	36.0	36.0	36.0	32.0	36.0
16	34.076421920058145	36.0	36.0	36.0	32.0	36.0
17	34.01075189140972	36.0	36.0	36.0	32.0	36.0
18	34.010046771978345	36.0	36.0	36.0	32.0	36.0
19	34.023953294097105	36.0	36.0	36.0	32.0	36.0
20	34.010835901623096	36.0	36.0	36.0	32.0	36.0
21	33.99614968920941	36.0	36.0	36.0	32.0	36.0
22	33.96005597534442	36.0	36.0	36.0	32.0	36.0
23	33.920835004884864	36.0	36.0	36.0	32.0	36.0
24	33.90292431057349	36.0	36.0	36.0	32.0	36.0
25	33.52772478631672	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	32.0
5	195.0
6	459.0
7	127.0
8	387.0
9	265.0
10	170.0
11	49.0
12	85.0
13	107.0
14	349.0
15	509.0
16	735.0
17	1071.0
18	1458.0
19	1928.0
20	3071.0
21	4475.0
22	6557.0
23	10034.0
24	14125.0
25	19960.0
26	26754.0
27	33762.0
28	45029.0
29	58510.0
30	75962.0
31	102693.0
32	144572.0
33	202995.0
34	436461.0
35	925902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.562043602697415	17.023902569795275	11.669186999237292	25.744866828270013
2	16.866592473237034	19.010414454314876	37.28734284632804	26.835650226120052
3	19.369523078643955	22.649575675023847	27.894771370465133	30.08612987586707
4	13.042991871760739	15.261548047240082	34.747986821112654	36.94747325988653
5	15.259463570852905	36.06640658381345	33.166465458916555	15.507664386417083
6	35.53121701367981	34.30605637694198	16.197142189193592	13.965584420184618
7	31.256077405235	29.809406901947888	19.74862576224482	19.18588993057229
8	28.55061842844473	33.02880864935152	18.60577306981199	19.814799852391758
9	27.146883911926835	14.658857052398291	18.188068679871183	40.006190355803696
10	16.530224706250433	26.656231479669817	30.70057535310915	26.112968460970603
11	37.221050561845544	21.36984061112461	21.61893004037423	19.790178786655613
12	24.796771022383545	23.25634263460375	28.171789927404717	23.775096415607987
13	29.468777115505674	19.37051383115275	24.88846606816425	26.27224298517733
14	23.597231699180245	19.314549740011074	24.669178034290944	32.41904052651773
15	25.302464377520533	26.886825224056878	21.775686490987862	26.035023907434724
16	26.087190743632938	25.913102239192128	22.965931295527522	25.033775721647416
17	24.408651033460707	26.10295194825939	24.531424407422357	24.95697261085755
18	25.0840723875424	25.228473239772036	25.22748095642884	24.45997341625672
19	25.82077599952755	25.094100277564518	24.75922754384929	24.325896179058642
20	25.895697223464982	24.58923146043635	24.46309189338325	25.05197942271542
21	26.484499749614976	24.39071402250631	24.33544034694861	24.789345880930107
22	26.136080147867602	24.108097943385456	24.611526264264196	25.144295644482746
23	24.77002377940422	24.35527771453189	25.10824494392425	25.766453562139635
24	24.973178183316193	24.76611517578624	24.924707581964213	25.335999058933357
25	25.142890346097214	24.475714428110294	24.805782978628923	25.57561224716357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	498.0
1	498.0
2	591.5
3	685.0
4	685.0
5	685.0
6	1304.5
7	1924.0
8	1924.0
9	1924.0
10	2409.0
11	2894.0
12	2894.0
13	2894.0
14	3953.5
15	5013.0
16	5013.0
17	5013.0
18	8426.5
19	11840.0
20	11840.0
21	11840.0
22	18556.0
23	25272.0
24	25272.0
25	25272.0
26	37619.5
27	49967.0
28	49967.0
29	49967.0
30	64169.5
31	78372.0
32	78372.0
33	78372.0
34	99361.5
35	120351.0
36	120351.0
37	120351.0
38	143534.0
39	166717.0
40	166717.0
41	166717.0
42	193135.0
43	219553.0
44	219553.0
45	219553.0
46	246036.5
47	272520.0
48	272520.0
49	272520.0
50	282926.0
51	293332.0
52	293332.0
53	293332.0
54	280265.0
55	267198.0
56	267198.0
57	267198.0
58	246244.0
59	225290.0
60	225290.0
61	225290.0
62	196582.5
63	167875.0
64	167875.0
65	167875.0
66	136231.5
67	104588.0
68	104588.0
69	104588.0
70	79234.0
71	53880.0
72	53880.0
73	53880.0
74	40072.0
75	26264.0
76	26264.0
77	26264.0
78	20378.0
79	14492.0
80	14492.0
81	14492.0
82	10055.5
83	5619.0
84	5619.0
85	5619.0
86	4128.5
87	2638.0
88	2638.0
89	2638.0
90	1773.0
91	908.0
92	908.0
93	908.0
94	570.0
95	232.0
96	232.0
97	232.0
98	550.0
99	868.0
100	868.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010383284799343022
2	1.8878699635169131E-4
3	6.135577381429966E-4
4	0.011799187271980706
5	0.03525597156867835
6	0.06772733494116925
7	0.1106291798620911
8	0.11218667258199254
9	0.12247556388315974
10	0.13554906338051437
11	0.14555477418715398
12	0.13923040980937232
13	0.13413316090787666
14	0.1201629231778515
15	0.13786170408582257
16	0.12389146635579742
17	0.12724243554103992
18	0.11605680600720222
19	0.10218096177535291
20	0.09849961534649494
21	0.0967533356302418
22	0.10864691640039834
23	0.08646444432907462
24	0.09642295838662633
25	0.09236403796506497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2118790.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.414677599335135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02863726859921	40.07630470419688
2	14.23749205335978	15.209820957066174
3	4.728786334291148	7.577597924469106
4	2.03200362689652	4.341552744454292
5	1.0647649260001917	2.8437037620690084
6	0.620592079612171	1.9889235511911025
7	0.4494645407608391	1.6805602475951413
8	0.29511207305149756	1.2610652990173745
9	0.23110823898710803	1.1110114868441823
>10	1.1741503429116151	11.187910929010842
>50	0.07499617677628015	2.7288673982597036
>100	0.05392309611251333	5.881194368637181
>500	0.006660328694020699	2.3814053858739634
>1k	0.0023089139472604915	1.7300812413150457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3301	0.15579646873923325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.439349817584564E-5
7	0.0	0.0	0.0	0.0	1.4159024726376847E-4
8	0.0	0.0	0.0	0.0	1.887869963516913E-4
9	0.0	0.0	0.0	0.0	1.887869963516913E-4
10	0.0	0.0	0.0	4.719674908792282E-5	1.887869963516913E-4
11	0.0	0.0	0.0	9.439349817584564E-5	2.359837454396141E-4
12	0.0	0.0	0.0	9.439349817584564E-5	4.719674908792282E-4
13	0.0	0.0	0.0	9.439349817584564E-5	5.663609890550739E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	795	0.0	15.528218	1
GCGTTAT	155	0.0	12.865687	1
TCGCGTA	150	1.8189894E-12	12.668352	9
CGCGTAA	150	1.8189894E-12	12.668053	10
GTATCAA	2350	0.0	12.486364	1
CGCATCG	255	0.0	11.922592	13
GTCTTAG	240	0.0	11.870128	1
CGTCGTA	245	0.0	11.633925	10
CCGTCGT	265	0.0	11.473225	9
AACCCGG	150	1.7644197E-10	11.401517	11
GTCTACG	60	0.005894827	11.078786	1
CGACCAT	415	0.0	10.989155	10
TAGAACC	185	5.456968E-12	10.7836895	4
AATCGAA	125	2.237648E-7	10.641166	7
CGTTATT	170	1.4006218E-10	10.615547	2
GCGTAAC	180	3.45608E-11	10.55696	11
CCGACCA	370	0.0	10.528428	9
TTAATAC	190	9.094947E-12	10.498916	3
CGAACGA	100	2.3997272E-5	10.449909	16
TAGGACT	220	0.0	10.363546	4
>>END_MODULE