FastQCFastQC Report
Thu 2 Feb 2017
SRR4061970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118790
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22450.025.3876551
GGTATCA10200.024.594831
TAGACCG752.0661828E-623.4657615
GTATACG808.9741356E-519.2551651
ATAGACG1254.8587026E-717.5993213
TCAACGC32650.017.1815724
CGTCTTA1307.326162E-716.92242415
CAACGCA33550.016.917385
GTCCTAA2900.016.6941341
ATCAACG33750.016.6867623
GTATAGT2251.8189894E-1216.6266821
CGTCGTA4700.016.38234510
AACGCAG34850.016.2863146
GTTATAG3000.016.1376611
ATACGCG1259.439789E-615.84125639
TATCAAC35650.015.7974262
TAGGACG1958.931238E-1015.7942624
ACGGACC6550.015.785658
ATACCGT5300.015.3578976
GAGCGAA6750.015.31792716
Produced by FastQC (version 0.11.5)