##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2118790
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28447038168011	32.0	32.0	32.0	32.0	32.0
2	31.457795251063107	32.0	32.0	32.0	32.0	32.0
3	31.579713421339537	32.0	32.0	32.0	32.0	32.0
4	31.65975061237782	32.0	32.0	32.0	32.0	32.0
5	31.634914739072773	32.0	32.0	32.0	32.0	32.0
6	35.28854534899636	36.0	36.0	36.0	36.0	36.0
7	35.29559229560268	36.0	36.0	36.0	36.0	36.0
8	35.24640006796332	36.0	36.0	36.0	36.0	36.0
9	35.321428268020895	36.0	36.0	36.0	36.0	36.0
10	35.21573162040599	36.0	36.0	36.0	36.0	36.0
11	35.32834872733966	36.0	36.0	36.0	36.0	36.0
12	35.2537490737638	36.0	36.0	36.0	36.0	36.0
13	35.28871478532559	36.0	36.0	36.0	36.0	36.0
14	35.25033155716234	36.0	36.0	36.0	36.0	36.0
15	35.22845586396009	36.0	36.0	36.0	36.0	36.0
16	35.24233831573681	36.0	36.0	36.0	36.0	36.0
17	35.22468531567546	36.0	36.0	36.0	36.0	36.0
18	35.219370017793175	36.0	36.0	36.0	36.0	36.0
19	35.21026765276408	36.0	36.0	36.0	36.0	36.0
20	35.21524549389038	36.0	36.0	36.0	36.0	36.0
21	35.20227252346859	36.0	36.0	36.0	36.0	36.0
22	35.191646175411435	36.0	36.0	36.0	36.0	36.0
23	35.16364104040514	36.0	36.0	36.0	36.0	36.0
24	35.14644679274492	36.0	36.0	36.0	36.0	36.0
25	35.12792301266289	36.0	36.0	36.0	36.0	36.0
26	35.086285568650034	36.0	36.0	36.0	36.0	36.0
27	35.077337536990456	36.0	36.0	36.0	36.0	36.0
28	35.06117737010275	36.0	36.0	36.0	36.0	36.0
29	35.04136511877062	36.0	36.0	36.0	36.0	36.0
30	35.020281858985555	36.0	36.0	36.0	36.0	36.0
31	35.02426951231599	36.0	36.0	36.0	36.0	36.0
32	35.00092269644467	36.0	36.0	36.0	36.0	36.0
33	34.979567583384856	36.0	36.0	36.0	36.0	36.0
34	34.97009189207047	36.0	36.0	36.0	36.0	36.0
35	34.95701225699574	36.0	36.0	36.0	36.0	36.0
36	34.94025977090698	36.0	36.0	36.0	36.0	36.0
37	34.927361843316234	36.0	36.0	36.0	36.0	36.0
38	34.912933325152565	36.0	36.0	36.0	36.0	36.0
39	34.90637580883429	36.0	36.0	36.0	36.0	36.0
40	34.88175279286763	36.0	36.0	36.0	32.0	36.0
41	34.86207458030291	36.0	36.0	36.0	32.0	36.0
42	34.832907933301556	36.0	36.0	36.0	32.0	36.0
43	34.82860783749215	36.0	36.0	36.0	32.0	36.0
44	34.78847030616531	36.0	36.0	36.0	32.0	36.0
45	34.77455387272925	36.0	36.0	36.0	32.0	36.0
46	34.76352446443489	36.0	36.0	36.0	32.0	36.0
47	34.73486848625867	36.0	36.0	36.0	32.0	36.0
48	34.71637160832362	36.0	36.0	36.0	32.0	36.0
49	34.702397122886175	36.0	36.0	36.0	32.0	36.0
50	34.29574474110223	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	8.0
20	18.0
21	43.0
22	145.0
23	396.0
24	943.0
25	2314.0
26	4800.0
27	8909.0
28	15390.0
29	24815.0
30	36897.0
31	55336.0
32	83936.0
33	144543.0
34	333963.0
35	1406331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23666158356664	17.47772799592093	12.147649105578978	26.137961314933456
2	16.353309997960988	19.45586669385351	37.00039458679777	27.19042872138774
3	18.813680273816917	23.08135594500252	28.159636546931548	29.945327234249014
4	12.929785396381895	15.644825584413747	34.798965447259995	36.626423571944365
5	14.90540115131804	36.45171841084695	32.96854948746667	15.674330950368349
6	35.65878228496669	34.47310443517709	16.085752798297897	13.782360481558326
7	31.306358818004615	29.761656417105993	19.814092005342673	19.117892759546724
8	28.563991712250857	33.09148145875712	18.546859292331945	19.797667536660075
9	27.225397514619193	14.373203573737841	18.10594726235257	40.2954516492904
10	16.479846025180432	26.566367187278768	30.840603212780138	26.11318357476067
11	37.524860887582065	21.23929223755068	21.5627787557993	19.673068119067956
12	24.73831762468201	23.207538264764324	28.305967084987188	23.74817702556648
13	29.570934354041693	19.22833315241246	24.93734631558578	26.26338617796006
14	23.72245479731356	19.05025981810373	24.757432308062622	32.46985307652009
15	25.315061898536428	26.82776490355344	21.84798871053762	26.00918448737251
16	26.13772001489539	25.717335991470552	23.067363861321883	25.07758013231218
17	24.45372122768184	25.974636467040153	24.573081806125195	24.99856049915282
18	25.178451413641035	25.057061031233008	25.350956020056785	24.413531535069172
19	25.823654066707885	24.895388405646617	24.933428985411485	24.347528542234013
20	26.000893910224793	24.33767340749233	24.655733650622693	25.005699031660182
21	26.597397571255293	24.184558167633412	24.48949636349048	24.72854789762081
22	26.11391407359861	24.05873163456501	24.756393979582686	25.070960312253693
23	24.845407048362507	24.115178946474167	25.240160657733895	25.799253347429428
24	25.11153913863891	24.557395046071694	25.028094222902702	25.30297159238669
25	25.27220827815226	24.24020026600188	25.003893746501525	25.483697709344334
26	25.06834147326848	24.938454916338333	25.15315475190346	24.84004885848973
27	25.54721374905075	24.564949907470293	24.66477155002952	25.223064793449435
28	25.123279886877015	24.514054473474054	25.152778328415355	25.209887311233576
29	25.014206261704825	24.754198634688578	25.143384129764996	25.0882109738416
30	25.05627104116883	24.77610756842554	25.162984250716104	25.004637139689528
31	25.52228070440404	24.492633510541893	24.62572871986951	25.359357065184557
32	25.33130008202857	24.532158691771752	24.465563575917017	25.670977650282662
33	24.90137026774332	24.411371999869736	24.97400630456257	25.713251427824368
34	25.587687945217247	24.433385612117224	24.958832227581205	25.020094215084328
35	25.799515193620493	24.36737298374917	25.067161164139428	24.765950658490908
36	24.91118739165381	24.81575542987568	24.81655777574622	25.45649940272429
37	25.779291667590954	24.544696380084304	24.7105488091805	24.96546314314424
38	25.16160182141866	24.38035329631846	25.02435354551753	25.433691336745344
39	25.70285730467533	24.330605348360848	24.58329793560445	25.383239411359366
40	25.77356761203462	24.555417793293994	24.811932487786645	24.859082106884742
41	25.007799362175003	24.83552903274222	25.15198726804272	25.004684337040057
42	25.876009891115604	24.719293103358762	24.731045765053437	24.673651240472203
43	25.59398872413785	24.095134482001807	24.92951996167434	25.381356832186004
44	25.1227651179036	24.601685955406197	24.703258632733586	25.572290293956613
45	25.210018591229417	24.81997563647468	24.78099022013545	25.18901555216046
46	25.184472672935165	24.6724235418942	24.76587522218373	25.377228562986904
47	25.297189636415553	24.625666366963774	24.988330466096993	25.08881353052368
48	25.845577875856833	25.055234367883312	24.229066988751118	24.87012076750874
49	25.28430249955869	25.020177524456106	24.3157341267781	25.379785849207103
50	25.30830605709485	25.1559052368018	24.50070914286928	25.03507956323407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	205.0
1	135.5
2	66.0
3	171.5
4	277.0
5	303.5
6	330.0
7	258.0
8	186.0
9	260.5
10	335.0
11	581.5
12	828.0
13	1614.5
14	2401.0
15	3047.5
16	3694.0
17	3587.5
18	3481.0
19	3301.5
20	3122.0
21	3723.0
22	4324.0
23	5352.0
24	6380.0
25	8216.0
26	10052.0
27	12875.5
28	15699.0
29	18571.5
30	21444.0
31	25091.0
32	28738.0
33	33555.5
34	38373.0
35	45080.0
36	51787.0
37	60562.5
38	69338.0
39	78341.0
40	87344.0
41	96148.0
42	104952.0
43	110559.5
44	116167.0
45	126824.5
46	137482.0
47	146934.5
48	156387.0
49	162722.5
50	169058.0
51	167644.5
52	166231.0
53	165355.5
54	164480.0
55	164326.0
56	164172.0
57	158147.0
58	152122.0
59	140055.5
60	127989.0
61	112427.5
62	96866.0
63	83325.0
64	69784.0
65	58614.5
66	47445.0
67	40441.5
68	33438.0
69	29294.5
70	25151.0
71	19710.0
72	14269.0
73	12187.5
74	10106.0
75	7511.0
76	4916.0
77	4288.5
78	3661.0
79	3078.0
80	2495.0
81	1946.0
82	1397.0
83	1174.5
84	952.0
85	736.0
86	520.0
87	357.0
88	194.0
89	126.5
90	59.0
91	44.0
92	29.0
93	26.0
94	23.0
95	20.0
96	17.0
97	16.0
98	15.0
99	12.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031149854398029062
2	0.005569216392374893
3	9.439349817584566E-5
4	0.0
5	4.719674908792283E-5
6	9.439349817584566E-5
7	0.0
8	0.0
9	0.0
10	9.439349817584566E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.135577381429966E-4
17	0.0
18	2.8318049452753693E-4
19	0.0
20	4.247707417913054E-4
21	0.0
22	0.0
23	0.0
24	0.0012743122253739162
25	3.7757399270338263E-4
26	8.495414835826108E-4
27	5.191642399671511E-4
28	0.001604689468989376
29	2.8318049452753693E-4
30	0.0012743122253739162
31	2.8318049452753693E-4
32	7.551479854067653E-4
33	3.3037724361545975E-4
34	9.911317308463792E-4
35	2.8318049452753693E-4
36	1.4159024726376847E-4
37	0.0018406732144289903
38	9.439349817584566E-5
39	4.719674908792282E-4
40	1.4159024726376847E-4
41	0.0012743122253739162
42	0.005710806639638661
43	0.004955658654231896
44	0.005191642399671511
45	0.0023126407053082184
46	0.0022654439562202955
47	0.0011799187271980705
48	0.004294904167000977
49	0.004530887912440591
50	0.0016518862180772986
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2118790.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.08983298014065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36292396283457	40.22278137064621
2	14.85906067806669	16.074482206368057
3	4.772217325612254	7.743853142619009
4	2.0334696835594346	4.399601422156372
5	1.0939618248107068	2.958610619533051
6	0.6562654972166542	2.129837468104667
7	0.42095408841136006	1.5938535434134478
8	0.296972555958058	1.2850556761165466
9	0.22268509014094387	1.0840499399602097
>10	1.1555411931303639	11.134921776611703
>50	0.06971648557242138	2.556277782540086
>100	0.04876594549175052	5.384564808362996
>500	0.005357715539812796	1.949912556745842
>1k	0.0021079536550082916	1.482197686821778
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.439349817584564E-5
2	0.0	0.0	0.0	0.0	9.439349817584564E-5
3	0.0	0.0	0.0	0.0	9.439349817584564E-5
4	0.0	0.0	0.0	0.0	9.439349817584564E-5
5	0.0	0.0	0.0	0.0	9.439349817584564E-5
6	0.0	0.0	0.0	0.0	1.4159024726376847E-4
7	0.0	0.0	0.0	0.0	1.4159024726376847E-4
8	0.0	0.0	0.0	0.0	1.4159024726376847E-4
9	0.0	0.0	0.0	0.0	1.4159024726376847E-4
10	0.0	0.0	0.0	0.0	2.359837454396141E-4
11	0.0	0.0	0.0	0.0	3.3037724361545975E-4
12	0.0	0.0	0.0	0.0	3.775739927033826E-4
13	0.0	0.0	0.0	0.0	3.775739927033826E-4
14	0.0	0.0	0.0	0.0	3.775739927033826E-4
15	0.0	0.0	0.0	0.0	7.079512363188424E-4
16	0.0	0.0	0.0	2.359837454396141E-4	7.079512363188424E-4
17	0.0	0.0	0.0	2.359837454396141E-4	7.079512363188424E-4
18	0.0	0.0	0.0	2.359837454396141E-4	8.495414835826108E-4
19	0.0	0.0	0.0	2.8318049452753693E-4	8.495414835826108E-4
20	0.0	0.0	0.0	4.719674908792282E-4	8.495414835826108E-4
21	0.0	0.0	0.0	7.079512363188424E-4	8.495414835826108E-4
22	0.0	0.0	0.0	8.967382326705337E-4	8.495414835826108E-4
23	0.0	0.0	0.0	0.0012743122253739162	8.495414835826108E-4
24	0.0	0.0	0.0	0.0014630992217256075	8.967382326705337E-4
25	0.0	0.0	0.0	0.0017462797162531446	8.967382326705337E-4
26	0.0	0.0	0.0	0.00217105045804445	9.439349817584564E-4
27	0.0	0.0	0.0	0.002690214698011601	0.001085525229022225
28	0.0	0.0	0.0	0.003398165934330443	0.001085525229022225
29	0.0	0.0	0.0	0.004483691163352668	0.001085525229022225
30	0.0	0.0	0.0	0.006560348123221272	0.001085525229022225
31	0.0	0.0	0.0	0.010147301053903407	0.001085525229022225
32	0.0	0.0	0.0	0.014111827977288924	0.0011327219781101477
33	0.0	0.0	0.0	0.017745977657058983	0.0011327219781101477
34	0.0	0.0	0.0	0.022748833060378802	0.0012271154762859935
35	0.0	0.0	0.0	0.02855403319819331	0.0012271154762859935
36	0.0	0.0	0.0	0.03501998782323874	0.0012271154762859935
37	0.0	0.0	0.0	0.04606402710981267	0.0012271154762859935
38	0.0	0.0	0.0	0.060034264839837835	0.0012271154762859935
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2245	0.0	25.387655	1
GGTATCA	1020	0.0	24.59483	1
TAGACCG	75	2.0661828E-6	23.465761	5
GTATACG	80	8.9741356E-5	19.255165	1
ATAGACG	125	4.8587026E-7	17.599321	3
TCAACGC	3265	0.0	17.181572	4
CGTCTTA	130	7.326162E-7	16.922424	15
CAACGCA	3355	0.0	16.91738	5
GTCCTAA	290	0.0	16.694134	1
ATCAACG	3375	0.0	16.686762	3
GTATAGT	225	1.8189894E-12	16.626682	1
CGTCGTA	470	0.0	16.382345	10
AACGCAG	3485	0.0	16.286314	6
GTTATAG	300	0.0	16.137661	1
ATACGCG	125	9.439789E-6	15.841256	39
TATCAAC	3565	0.0	15.797426	2
TAGGACG	195	8.931238E-10	15.794262	4
ACGGACC	655	0.0	15.78565	8
ATACCGT	530	0.0	15.357897	6
GAGCGAA	675	0.0	15.317927	16
>>END_MODULE