FastQCFastQC Report
Thu 2 Feb 2017
SRR4061969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747468
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT25050.33513140361861643No Hit
CTTTAGGACGTGAAATATGGCGAGG19730.2639577881594931No Hit
GTCCTACAGTGGACATTTCTAAATT18800.251515783953293No Hit
CTGTAGGACGTGGAATATGGCAAGA18030.24121433961052513No Hit
GTCCTACAGTGTGCATTTCTCATTT16790.22462500066892493No Hit
CCATAGGGTCTTCTCGTCTTATTAT12760.1707096491087244No Hit
GTATCAACGCAGAGTACTTTTTTTT11310.15131082534636933No Hit
CTGAAGGACCTGGAATATGGCGAGA9820.13137686161815623No Hit
GTGTAAGCATCTGGGTAGTCTGAGT9290.1242862570705368No Hit
CTGTAGGACCTGGAATATGGCGAGA9060.12120920226685289No Hit
ATTTAGAAATGTCCACTGTAGGACG8660.11585780260827219No Hit
GGATATGAGATTGGCTTGAAACCAA8240.11023883296676246No Hit
CATTTACACCTACTACCCAACTATC8050.10769691812893663No Hit
GAATATGGCAAGAAAACTGAAAATC7920.10595771323989789No Hit
GTCCTTCAGTGTGCATTTCTCATTT7800.10435229334232368No Hit
GTATTGGAATTAGTGAAATTGGAGT7620.10194416349596237No Hit
CTATGTAGTAGCCCATTTCCACTAT7510.10047252858985267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTC350.002174456116.27934317
CACCCGT508.738366E-515.19303618
CGGATTA456.7422446E-414.7818969
GGTTTGA902.2791937E-914.77793515
ACCCGTA456.783469E-414.77001819
GGTATCA2650.014.3330531
TACGGGT400.005275330514.2501524
CCAAGCG400.005291767414.24347118
CGGTTTG1101.8553692E-1013.81832714
TAGGACC3050.013.7050634
GTATCAA11650.013.6932941
GGGTCTT2550.013.4146036
TGAACGG500.001497397813.3010335
CTACACT1001.00371835E-813.3001414
CGTCTTA2300.013.21753115
GTATATA1153.783498E-1013.2113361
CACCTTT2600.013.15398614
GGTCGGT1051.9776053E-812.67104611
TTCCACC2850.012.67104511
ATCGGAT604.0756958E-412.6710457
Produced by FastQC (version 0.11.5)