##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061969_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747468
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389052106578475	32.0	32.0	32.0	32.0	32.0
2	31.08695756875211	32.0	32.0	32.0	32.0	32.0
3	31.096230206510512	32.0	32.0	32.0	32.0	32.0
4	31.092650120138924	32.0	32.0	32.0	32.0	32.0
5	31.10205921858862	32.0	32.0	32.0	32.0	32.0
6	34.73790717462152	36.0	36.0	36.0	32.0	36.0
7	34.67607308941653	36.0	36.0	36.0	32.0	36.0
8	34.64900838564326	36.0	36.0	36.0	32.0	36.0
9	34.71529483536419	36.0	36.0	36.0	32.0	36.0
10	34.525627852964945	36.0	36.0	36.0	32.0	36.0
11	34.703802704597386	36.0	36.0	36.0	32.0	36.0
12	34.58121016551879	36.0	36.0	36.0	32.0	36.0
13	34.641505723321934	36.0	36.0	36.0	32.0	36.0
14	34.5921912376182	36.0	36.0	36.0	32.0	36.0
15	34.55931892736545	36.0	36.0	36.0	32.0	36.0
16	34.55363306522821	36.0	36.0	36.0	32.0	36.0
17	34.54178239068428	36.0	36.0	36.0	32.0	36.0
18	34.53301144664387	36.0	36.0	36.0	32.0	36.0
19	34.47986402093468	36.0	36.0	36.0	32.0	36.0
20	34.47685118292689	36.0	36.0	36.0	32.0	36.0
21	34.44508527455356	36.0	36.0	36.0	32.0	36.0
22	34.39755815633579	36.0	36.0	36.0	32.0	36.0
23	34.40467819358153	36.0	36.0	36.0	32.0	36.0
24	34.34177249059491	36.0	36.0	36.0	32.0	36.0
25	34.02736973355381	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	60.0
6	170.0
7	30.0
8	142.0
9	103.0
10	47.0
11	17.0
12	27.0
13	41.0
14	88.0
15	127.0
16	206.0
17	266.0
18	370.0
19	457.0
20	708.0
21	970.0
22	1404.0
23	2026.0
24	3004.0
25	4268.0
26	5971.0
27	8027.0
28	11277.0
29	15355.0
30	20467.0
31	28464.0
32	41621.0
33	62837.0
34	154586.0
35	384326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.72581663669835	22.591563918143677	13.256967634215258	24.425651810942718
2	13.558342453034653	22.21238156651941	42.83097826523213	21.3982977152138
3	16.74136348676729	28.144959101818678	30.623466485432115	24.490210925981916
4	10.664163737588492	18.719385167870634	41.285618906302055	29.33083218823882
5	11.326577589344982	39.737104130532536	36.85614333740177	12.080174942720713
6	27.616970631666177	41.5697740578156	19.198324946014584	11.614930364503635
7	26.01982733450658	32.78514851750361	24.16526707435652	17.029757073633288
8	23.048775751016915	38.941310249778674	21.34201957887603	16.667894420328384
9	25.250285983717013	16.24561647909171	22.856863475258056	35.647234061933226
10	15.052528917028157	28.61988677010795	34.7750540561541	21.552530256709794
11	31.121507723101782	24.777698731372176	27.022750627347108	17.07804291817893
12	22.233281934696492	27.480631734348982	31.83911110277539	18.446975228179134
13	26.663719540784204	23.973931331966938	27.65850848638294	21.703840640865916
14	19.848670745689702	23.992209851805022	28.725769638781433	27.43334976372384
15	22.21626813744469	33.611895189650255	22.918120765808432	21.25371590709663
16	21.1294773220457	29.090470704851718	29.095426902778147	20.68462507032443
17	18.498921677628495	31.126411530681956	29.41783987247666	20.956826919212894
18	18.966955930078527	29.780916115396412	31.811296140282263	19.440831814242795
19	22.36328621974378	27.83772997672444	28.50439128653044	21.294592517001337
20	22.262782030903715	29.441287891468736	28.73812721704512	19.557802860582427
21	21.743567081132113	28.298549036150227	27.28441435831509	22.673469524402574
22	21.826640935431296	30.254334780157304	28.050751781521992	19.868272502889404
23	20.325191277171804	30.617201957092853	29.140277067360092	19.917329698375248
24	21.88857121813714	28.89100173416984	29.280687775776528	19.939739271916494
25	20.97753674132101	29.451441197147737	29.702520839610326	19.868501221920926
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	64.0
2	187.0
3	310.0
4	310.0
5	310.0
6	771.0
7	1232.0
8	1232.0
9	1232.0
10	1348.0
11	1464.0
12	1464.0
13	1464.0
14	2494.5
15	3525.0
16	3525.0
17	3525.0
18	7615.5
19	11706.0
20	11706.0
21	11706.0
22	16840.0
23	21974.0
24	21974.0
25	21974.0
26	35536.0
27	49098.0
28	49098.0
29	49098.0
30	64901.5
31	80705.0
32	80705.0
33	80705.0
34	88777.5
35	96850.0
36	96850.0
37	96850.0
38	100652.0
39	104454.0
40	104454.0
41	104454.0
42	100416.0
43	96378.0
44	96378.0
45	96378.0
46	96611.5
47	96845.0
48	96845.0
49	96845.0
50	85251.0
51	73657.0
52	73657.0
53	73657.0
54	60900.0
55	48143.0
56	48143.0
57	48143.0
58	37779.0
59	27415.0
60	27415.0
61	27415.0
62	21643.0
63	15871.0
64	15871.0
65	15871.0
66	12243.5
67	8616.0
68	8616.0
69	8616.0
70	6599.5
71	4583.0
72	4583.0
73	4583.0
74	3570.0
75	2557.0
76	2557.0
77	2557.0
78	1829.5
79	1102.0
80	1102.0
81	1102.0
82	769.5
83	437.0
84	437.0
85	437.0
86	292.5
87	148.0
88	148.0
89	148.0
90	111.5
91	75.0
92	75.0
93	75.0
94	58.0
95	41.0
96	41.0
97	41.0
98	129.5
99	218.0
100	218.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0016054198975742106
2	2.675699829290351E-4
3	2.675699829290351E-4
4	0.010301444342767852
5	0.03371381784905842
6	0.0678289906725104
7	0.10997126298383342
8	0.11331588777044636
9	0.12334976213028517
10	0.13672826127673693
11	0.14328372585849827
12	0.1357917663364853
13	0.13084172165229815
14	0.11826593245463352
15	0.1357917663364853
16	0.12415247207907228
17	0.12549032199371746
18	0.11492130766802057
19	0.10194416349596237
20	0.09324813905076873
21	0.09672654882884618
22	0.1036833683850011
23	0.08642510448607833
24	0.09538869891420101
25	0.09271299908491067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	747468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.23814130125625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.099820440012714	12.697938752213625
2	13.996222678546713	6.224999552187381
3	7.326157245774902	4.8876036008029375
4	4.645763187572329	4.1325255286963225
5	3.094502992256316	3.440799739947811
6	2.3269047950742507	3.104762257645914
7	1.663710257069846	2.5898476646748017
8	1.3053641825970295	2.322309851375328
9	1.0134410015066508	2.0283339772792393
>10	6.4818993607596385	26.726012704612064
>50	0.6104923035682193	9.466762449098447
>100	0.4043833521167376	16.815072613516875
>500	0.027119598875191043	3.9216898054207943
>1k	0.0042186042694741625	1.641341502528483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	2505	0.33513140361861643	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1973	0.2639577881594931	No Hit
GTCCTACAGTGGACATTTCTAAATT	1880	0.251515783953293	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1803	0.24121433961052513	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1679	0.22462500066892493	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1276	0.1707096491087244	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1131	0.15131082534636933	No Hit
CTGAAGGACCTGGAATATGGCGAGA	982	0.13137686161815623	No Hit
GTGTAAGCATCTGGGTAGTCTGAGT	929	0.1242862570705368	No Hit
CTGTAGGACCTGGAATATGGCGAGA	906	0.12120920226685289	No Hit
ATTTAGAAATGTCCACTGTAGGACG	866	0.11585780260827219	No Hit
GGATATGAGATTGGCTTGAAACCAA	824	0.11023883296676246	No Hit
CATTTACACCTACTACCCAACTATC	805	0.10769691812893663	No Hit
GAATATGGCAAGAAAACTGAAAATC	792	0.10595771323989789	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	780	0.10435229334232368	No Hit
GTATTGGAATTAGTGAAATTGGAGT	762	0.10194416349596237	No Hit
CTATGTAGTAGCCCATTTCCACTAT	751	0.10047252858985267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.675699829290351E-4	0.0
8	0.0	0.0	0.0	2.675699829290351E-4	0.0
9	0.0	0.0	0.0	4.0135497439355264E-4	0.0
10	0.0	0.0	0.0	4.0135497439355264E-4	0.0
11	0.0	0.0	0.0	6.689249573225877E-4	0.0
12	0.0	0.0	0.0	6.689249573225877E-4	0.0
13	0.0	0.0	0.0	6.689249573225877E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGTC	35	0.0021744561	16.279343	17
CACCCGT	50	8.738366E-5	15.193036	18
CGGATTA	45	6.7422446E-4	14.781896	9
GGTTTGA	90	2.2791937E-9	14.777935	15
ACCCGTA	45	6.783469E-4	14.770018	19
GGTATCA	265	0.0	14.333053	1
TACGGGT	40	0.0052753305	14.250152	4
CCAAGCG	40	0.0052917674	14.243471	18
CGGTTTG	110	1.8553692E-10	13.818327	14
TAGGACC	305	0.0	13.705063	4
GTATCAA	1165	0.0	13.693294	1
GGGTCTT	255	0.0	13.414603	6
TGAACGG	50	0.0014973978	13.301033	5
CTACACT	100	1.00371835E-8	13.300141	4
CGTCTTA	230	0.0	13.217531	15
GTATATA	115	3.783498E-10	13.211336	1
CACCTTT	260	0.0	13.153986	14
GGTCGGT	105	1.9776053E-8	12.671046	11
TTCCACC	285	0.0	12.671045	11
ATCGGAT	60	4.0756958E-4	12.671045	7
>>END_MODULE