FastQCFastQC Report
Thu 2 Feb 2017
SRR4061969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747468
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG12120.16214740965499527No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC9660.12923630175472395No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9640.1289687317717949No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9080.12147677224978193No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC8550.1143861677021625No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8330.11144289788994312No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT8090.10823205809479469No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8050.10769691812893663No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA7530.10074009857278171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG250.002352009535.2006429
TCTAGCG300.00574499429.33190516
TAGGACC3250.025.7218254
GTATCAA10550.025.6549071
GGTATCA5050.023.9656411
CGCGGTA2300.022.00187343
GGGGTCC1700.021.998936
TATAGCG500.002580253621.998935
ACGACAG500.002580253621.998935
GTTATAT1351.1277734E-1021.1898231
GTCCTAC12850.021.0629791
AACGGCC2800.020.42894437
GGGTCCT2050.020.3892527
AGGACCT7050.020.2827015
CGGCCGC2500.020.24172239
TTAGGAC9100.020.0676443
GGACCTG7150.019.9990276
TCCTACA14050.019.8878142
TAACGGC2800.019.64321536
GTCTAAA909.992302E-619.5598371
Produced by FastQC (version 0.11.5)