##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747468
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32439515805359	32.0	32.0	32.0	32.0	32.0
2	31.55051052352743	32.0	32.0	32.0	32.0	32.0
3	31.626719806065278	32.0	32.0	32.0	32.0	32.0
4	31.69607394564048	32.0	32.0	32.0	32.0	32.0
5	31.680922795357127	32.0	32.0	32.0	32.0	32.0
6	35.32296098294509	36.0	36.0	36.0	36.0	36.0
7	35.32628286428315	36.0	36.0	36.0	36.0	36.0
8	35.2759435855448	36.0	36.0	36.0	36.0	36.0
9	35.3642711126095	36.0	36.0	36.0	36.0	36.0
10	35.267602091326985	36.0	36.0	36.0	36.0	36.0
11	35.36621768423531	36.0	36.0	36.0	36.0	36.0
12	35.30538297291657	36.0	36.0	36.0	36.0	36.0
13	35.33419892222811	36.0	36.0	36.0	36.0	36.0
14	35.300721903813944	36.0	36.0	36.0	36.0	36.0
15	35.28999234749849	36.0	36.0	36.0	36.0	36.0
16	35.30360898392975	36.0	36.0	36.0	36.0	36.0
17	35.28977160226257	36.0	36.0	36.0	36.0	36.0
18	35.294124965884826	36.0	36.0	36.0	36.0	36.0
19	35.24724001562609	36.0	36.0	36.0	36.0	36.0
20	35.261328913077215	36.0	36.0	36.0	36.0	36.0
21	35.23207816254342	36.0	36.0	36.0	36.0	36.0
22	35.191826272161485	36.0	36.0	36.0	36.0	36.0
23	35.20630314608786	36.0	36.0	36.0	36.0	36.0
24	35.1728542225219	36.0	36.0	36.0	36.0	36.0
25	35.151254635649956	36.0	36.0	36.0	36.0	36.0
26	35.11517683700172	36.0	36.0	36.0	36.0	36.0
27	35.07670562485618	36.0	36.0	36.0	36.0	36.0
28	35.04747226637127	36.0	36.0	36.0	36.0	36.0
29	35.018549289066556	36.0	36.0	36.0	36.0	36.0
30	34.981664766919785	36.0	36.0	36.0	36.0	36.0
31	34.98965440661005	36.0	36.0	36.0	36.0	36.0
32	34.96036084487898	36.0	36.0	36.0	36.0	36.0
33	34.988716573819886	36.0	36.0	36.0	36.0	36.0
34	34.95000187298988	36.0	36.0	36.0	36.0	36.0
35	34.93880540705422	36.0	36.0	36.0	36.0	36.0
36	34.89180138815307	36.0	36.0	36.0	36.0	36.0
37	34.834684561747125	36.0	36.0	36.0	36.0	36.0
38	34.827395955412136	36.0	36.0	36.0	36.0	36.0
39	34.692111234193305	36.0	36.0	36.0	32.0	36.0
40	34.74453488309867	36.0	36.0	36.0	32.0	36.0
41	34.7419608598629	36.0	36.0	36.0	32.0	36.0
42	34.72741441774096	36.0	36.0	36.0	32.0	36.0
43	34.741841791220494	36.0	36.0	36.0	32.0	36.0
44	34.629432965692175	36.0	36.0	36.0	32.0	36.0
45	34.63438568607619	36.0	36.0	36.0	32.0	36.0
46	34.51804491964874	36.0	36.0	36.0	32.0	36.0
47	34.55423242198997	36.0	36.0	36.0	32.0	36.0
48	34.520156046814044	36.0	36.0	36.0	32.0	36.0
49	34.454755253736614	36.0	36.0	36.0	32.0	36.0
50	34.0933511534942	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	15.0
22	38.0
23	99.0
24	293.0
25	735.0
26	1754.0
27	3231.0
28	5963.0
29	9439.0
30	14145.0
31	20653.0
32	30850.0
33	51315.0
34	115742.0
35	493189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.39990792993575	23.211692898159537	13.519043648972174	24.869355522932537
2	13.091671940243794	22.782120767369022	42.49415000862949	21.632057283757693
3	16.30350584977223	28.527222010395135	30.939642658853593	24.229629480979042
4	10.502389399947557	19.177008246506873	41.35561656151167	28.964985792033904
5	11.14188165914795	39.950205226176905	36.69789208367448	12.210021031000657
6	27.63831398351229	41.69326765364578	19.14053615816639	11.52788220467553
7	25.959372173792055	32.767556604429885	24.338700787190888	16.93437043458717
8	23.251697731541686	38.87149683999851	21.302182835920735	16.57462259253908
9	25.24455896439714	16.068781539811738	22.88793633974966	35.79872315604146
10	15.006950139604825	28.78454834795382	34.68514329060681	21.523358221834542
11	31.287359458866465	24.70299731894877	26.96436502967351	17.04527819251125
12	22.12736866327388	27.423648905371202	31.938892367298667	18.510090064056254
13	26.650639224689215	23.84289360882339	27.81109024065244	21.695376925834953
14	19.959784231565767	23.924903808591136	28.600956830258955	27.514355129584146
15	22.22008166235879	33.603177661117265	23.016236146564133	21.160504529959812
16	21.177263860905306	29.214327952457786	28.895114400464507	20.713293786172407
17	18.6109639476205	31.18220445557536	29.197771677182168	21.00905991962198
18	19.104146543120358	29.6305116219333	31.668597635210165	19.596744199736175
19	22.353866653823307	27.809083465780475	28.653801901887437	21.183247978508778
20	22.456793790185987	29.452734720962404	28.584195584524696	19.50627590432691
21	21.82354294765796	28.128294455414814	27.39903246694173	22.649130129985497
22	21.893512498193903	30.11781106348365	27.99076348418929	19.997912954133152
23	20.529763226688573	30.592428284363436	28.957036172882777	19.920772316065214
24	22.10923170162297	28.837244149161755	29.195793202819694	19.85773094639558
25	21.13000911081886	29.35115182958889	29.48895129257234	20.029887767019904
26	19.622591024328898	30.14990668459393	30.420287237529514	19.807215053547658
27	19.960426187749512	30.85526527030064	28.58304881572892	20.601259726220928
28	19.60770935011559	29.01120601078859	29.415109384365103	21.96597525473071
29	20.75300048296705	28.660816655807714	28.083262984254738	22.5029198769705
30	20.28456562602431	28.88749155467553	28.887223980038932	21.940718839261226
31	22.212232862673957	28.023230612392336	29.093385349355554	20.671151175578157
32	20.12386013357308	29.829849516225703	28.84878842366641	21.197501926534805
33	19.881520011559022	30.510202443449085	29.887567093173217	19.720710451818675
34	20.74155039527146	28.997365748848438	30.734580131939992	19.52650372394011
35	20.53265156501683	30.390732446071272	28.82946159568035	20.24715439323155
36	19.834213194981444	29.1415261697522	29.94223148611442	21.082029149151936
37	21.406697143499322	28.322048342838947	27.83867346843213	22.4325810452296
38	20.577469537157445	30.484515725087896	28.749725740767495	20.18828899698716
39	21.747564762402956	28.97748784889687	28.187214617981095	21.087732770719086
40	22.346270805266318	28.11896712497007	29.485047500424766	20.049714569338846
41	22.328508529587655	27.878259217587097	29.839977416761098	19.95325483606415
42	21.059919671837246	29.965455143865544	28.874121327100454	20.100503857196756
43	19.547999015295037	30.495081826841776	29.46288090421604	20.494038253647155
44	19.601652045590466	29.789400310661634	29.257449122802328	21.351498520945576
45	19.89874962205032	30.053354453919574	29.131424810088646	20.91647111394146
46	21.32584119339086	28.54920061542578	28.366312127901534	21.758646063281827
47	22.15271330067187	29.23477455830767	28.222019463092636	20.390492677927828
48	19.97816535462568	29.841217174447042	27.83489154924301	22.345725921684263
49	21.063299471400647	28.262338333018928	29.309101514890017	21.365260680690405
50	19.694991263676425	29.929868593920162	28.49874373540044	21.876396407002975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	3.0
2	6.0
3	29.0
4	52.0
5	56.0
6	60.0
7	54.5
8	49.0
9	75.5
10	102.0
11	138.5
12	175.0
13	435.5
14	696.0
15	865.5
16	1035.0
17	1369.0
18	1703.0
19	1902.5
20	2102.0
21	2844.0
22	3586.0
23	4490.5
24	5395.0
25	8206.5
26	11018.0
27	15735.0
28	20452.0
29	23660.0
30	26868.0
31	31028.0
32	35188.0
33	43283.0
34	51378.0
35	59719.5
36	68061.0
37	70763.0
38	73465.0
39	74551.5
40	75638.0
41	72957.5
42	70277.0
43	60858.0
44	51439.0
45	47894.0
46	44349.0
47	42794.5
48	41240.0
49	37442.5
50	33645.0
51	32615.0
52	31585.0
53	28243.0
54	24901.0
55	22366.5
56	19832.0
57	17644.5
58	15457.0
59	13506.0
60	11555.0
61	9705.5
62	7856.0
63	6457.0
64	5058.0
65	4578.0
66	4098.0
67	3328.5
68	2559.0
69	2435.5
70	2312.0
71	1847.5
72	1383.0
73	1238.0
74	1093.0
75	946.5
76	800.0
77	633.5
78	467.0
79	341.5
80	216.0
81	167.5
82	119.0
83	92.0
84	65.0
85	57.0
86	49.0
87	47.5
88	46.0
89	34.0
90	22.0
91	13.5
92	5.0
93	4.5
94	4.0
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0282286331990132
2	0.004147334735400044
3	4.0135497439355264E-4
4	0.0
5	0.0
6	2.675699829290351E-4
7	0.0
8	0.0
9	0.0
10	1.3378499146451755E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.027099487871053E-4
17	0.0
18	2.675699829290351E-4
19	0.0
20	8.027099487871053E-4
21	0.0
22	0.0
23	2.675699829290351E-4
24	0.001471634906109693
25	6.689249573225877E-4
26	4.0135497439355264E-4
27	5.351399658580702E-4
28	0.0016054198975742106
29	6.689249573225877E-4
30	0.001739204889038728
31	8.027099487871053E-4
32	0.0016054198975742106
33	0.0
34	9.364949402516228E-4
35	0.0
36	2.675699829290351E-4
37	0.0020067748719677634
38	0.0
39	6.689249573225877E-4
40	1.3378499146451755E-4
41	0.001471634906109693
42	0.004548689709793597
43	0.004816259692722632
44	0.004147334735400044
45	0.0018729898805032457
46	0.002408129846361316
47	2.675699829290351E-4
48	0.0042811197268645615
49	0.0028094848207548687
50	0.0018729898805032457
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	747468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.25472254091324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.31741125999467	14.980447632164081
2	13.609020824548743	6.8738408995497755
3	7.0008015525577685	5.304099023215232
4	4.342967462506644	4.387217530792773
5	2.9841108852231053	3.768144621881426
6	2.1246620167202614	3.2194649835292424
7	1.6276178073697374	2.8773525289440607
8	1.2187559542848285	2.4623474776439425
9	0.9789097657016012	2.224988507284589
>10	5.961897339675812	27.514008156148538
>50	0.5088946765196062	8.886216628254852
>100	0.3090474962562857	14.729455960570908
>500	0.015372860019609408	2.6101596275653582
>1k	5.300986213658417E-4	0.16225642245520924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1212	0.16214740965499527	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	966	0.12923630175472395	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	964	0.1289687317717949	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	908	0.12147677224978193	No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC	855	0.1143861677021625	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	833	0.11144289788994312	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	809	0.10823205809479469	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	805	0.10769691812893663	No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA	753	0.10074009857278171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	1.3378499146451755E-4	0.0
22	0.0	0.0	0.0	1.3378499146451755E-4	0.0
23	0.0	0.0	0.0	1.3378499146451755E-4	0.0
24	0.0	0.0	0.0	4.0135497439355264E-4	0.0
25	0.0	0.0	0.0	8.027099487871053E-4	0.0
26	0.0	0.0	0.0	9.364949402516228E-4	0.0
27	0.0	0.0	0.0	0.001204064923180658	0.0
28	0.0	0.0	0.0	0.001204064923180658	0.0
29	0.0	0.0	0.0	0.0018729898805032457	0.0
30	0.0	0.0	0.0	0.0030770548036839034	0.0
31	0.0	0.0	0.0	0.00602032461590329	0.0
32	0.0	0.0	0.0	0.011505509265948509	0.0
33	0.0	0.0	0.0	0.016723123933064694	0.0
34	0.0	0.0	0.0	0.022475878566038946	0.0
35	0.0	0.0	0.0	0.02983405309658741	0.0
36	0.0	0.0	0.0	0.04053685241374882	0.0
37	0.0	0.0	0.0	0.054049136551665086	0.0
38	0.0	0.0	0.0	0.07572230516891693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGG	25	0.0023520095	35.20064	29
TCTAGCG	30	0.005744994	29.331905	16
TAGGACC	325	0.0	25.721825	4
GTATCAA	1055	0.0	25.654907	1
GGTATCA	505	0.0	23.965641	1
CGCGGTA	230	0.0	22.001873	43
GGGGTCC	170	0.0	21.99893	6
TATAGCG	50	0.0025802536	21.99893	5
ACGACAG	50	0.0025802536	21.99893	5
GTTATAT	135	1.1277734E-10	21.189823	1
GTCCTAC	1285	0.0	21.062979	1
AACGGCC	280	0.0	20.428944	37
GGGTCCT	205	0.0	20.389252	7
AGGACCT	705	0.0	20.282701	5
CGGCCGC	250	0.0	20.241722	39
TTAGGAC	910	0.0	20.067644	3
GGACCTG	715	0.0	19.999027	6
TCCTACA	1405	0.0	19.887814	2
TAACGGC	280	0.0	19.643215	36
GTCTAAA	90	9.992302E-6	19.559837	1
>>END_MODULE