FastQCFastQC Report
Thu 2 Feb 2017
SRR4061968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38814
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1050.2705209460503942No Hit
ATATAATCCGCGCCTTTCGTCTCGG810.20868758695316122No Hit
TATCAACGCAGAGTACTTTTTTTTT730.18807646725408358No Hit
TCATACAACTGCTCCGCATTCTGCT610.1571597877054671No Hit
CTCTAGGCCTGATTGAAATTCTCTT530.13654866800638946No Hit
GTATCAACGCAGAGTACATGGGATC510.13139588808162003No Hit
GCGTATGCAAGAGCCTTGATGAGGA480.12366671819446591No Hit
GTCGTAGGTAGCGCAGGCGACGTGG430.11078476838254236No Hit
ATTATACACCTTGGCTTGCAAACCA430.11078476838254236No Hit
GGTATCAACGCAGAGTACTTTTTTT430.11078476838254236No Hit
TGCATACGCTGCTCGCAAGGTTCCA420.10820837842015767No Hit
ATTGTACATCAACTTCACACCGGAG420.10820837842015767No Hit
GTATAATACTGCTACTGCCTCTGAG410.10563198845777297No Hit
GTCTACAACATCCCCAAAATTCGAC400.10305559849538826No Hit
ACCTACGACTGCTCTTCCAGCGGTC400.10305559849538826No Hit
TACCAGTACTGTACAGAATGGGGCT390.10047920853300356No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)