##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061968_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38814
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.79708352656258	32.0	32.0	32.0	32.0	32.0
2	30.08540732725305	32.0	32.0	32.0	21.0	32.0
3	30.08584531354666	32.0	32.0	32.0	21.0	32.0
4	30.05485134229917	32.0	32.0	32.0	21.0	32.0
5	30.076570309682072	32.0	32.0	32.0	21.0	32.0
6	33.32477971865821	36.0	36.0	36.0	21.0	36.0
7	33.341397433915596	36.0	36.0	36.0	21.0	36.0
8	33.12090998093471	36.0	32.0	36.0	21.0	36.0
9	33.43244705518627	36.0	32.0	36.0	21.0	36.0
10	32.8580409130726	36.0	32.0	36.0	21.0	36.0
11	33.48052249188437	36.0	36.0	36.0	21.0	36.0
12	33.131576235378986	36.0	32.0	36.0	21.0	36.0
13	33.321842634101095	36.0	32.0	36.0	21.0	36.0
14	33.06755294481373	36.0	32.0	36.0	21.0	36.0
15	32.95395991137219	36.0	32.0	36.0	21.0	36.0
16	32.97454526717164	36.0	32.0	36.0	21.0	36.0
17	32.93167413819756	36.0	32.0	36.0	21.0	36.0
18	32.86790848662854	36.0	32.0	36.0	21.0	36.0
19	32.91590663162776	36.0	32.0	36.0	21.0	36.0
20	32.84822486731591	36.0	32.0	36.0	21.0	36.0
21	32.84758076982532	36.0	32.0	36.0	21.0	36.0
22	32.750296284845675	36.0	32.0	36.0	21.0	36.0
23	32.77392178080074	36.0	32.0	36.0	21.0	36.0
24	32.755139897974956	36.0	32.0	36.0	21.0	36.0
25	31.87141237697738	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	7.0
7	4.0
8	7.0
9	6.0
10	1.0
11	0.0
12	1.0
13	1.0
14	26.0
15	50.0
16	56.0
17	67.0
18	70.0
19	91.0
20	115.0
21	167.0
22	177.0
23	254.0
24	357.0
25	518.0
26	686.0
27	973.0
28	1174.0
29	1628.0
30	2244.0
31	3171.0
32	4517.0
33	6012.0
34	9543.0
35	6889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.843149379090015	18.789611995671667	12.325449580048437	27.04178904518988
2	16.749111145462976	19.554799814499923	37.63848096047818	26.057608079558918
3	18.836472315976604	23.83737407569629	26.552959060108726	30.77319454821838
4	15.013397918169638	15.301968463361849	32.8223229928888	36.86231062557972
5	14.753253446720782	35.94897564746811	31.93402911995877	17.363741785852337
6	32.263469966486205	33.838618200567154	16.215519463779323	17.68239236916731
7	30.050544122956314	28.32276032802104	20.555469596162773	21.07122595285987
8	26.008922354882795	32.63364191959566	19.01028907398334	22.347146651538203
9	26.000051582286645	13.893688907229256	18.007376266989915	42.098883243494186
10	18.306807336136405	24.778806717053165	29.073696700802227	27.840689246008203
11	35.72036630981555	21.74642074035857	20.815168321939893	21.71804462788598
12	23.643210895583987	22.077486586875775	26.658584399504747	27.62071811803549
13	29.707490713990918	18.786112257531983	23.973380107304994	27.5330169211721
14	22.788466498168876	18.685201423634396	23.07474080569454	35.45159127250219
15	26.83933546589619	25.58559488184914	20.457125167681355	27.117944484573314
16	25.137330754352032	26.597034171502255	21.4468085106383	26.818826563507415
17	23.238237721832437	24.92519603796946	25.00257944696657	26.833986793231528
18	23.850419084461638	24.595744680851066	23.93810444874275	27.615731785944554
19	25.919240884946625	24.01629621989583	23.982775514413905	26.081687380743645
20	25.677452623436896	23.717932190279747	22.79747324996777	27.80714193631559
21	26.298960831334934	23.681699801449163	22.864289213790258	27.15505015342565
22	26.24113475177305	22.772404900064476	23.12830431979368	27.858156028368796
23	25.34612112305669	23.311934411013997	23.652254621393766	27.689689844535543
24	23.654694066988114	25.016115308253617	22.31390042028724	29.015290204471032
25	25.53822354002836	24.393451076447082	23.02694340595591	27.041381977568648
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	7.0
2	9.5
3	12.0
4	12.0
5	12.0
6	23.0
7	34.0
8	34.0
9	34.0
10	32.0
11	30.0
12	30.0
13	30.0
14	24.0
15	18.0
16	18.0
17	18.0
18	41.5
19	65.0
20	65.0
21	65.0
22	128.5
23	192.0
24	192.0
25	192.0
26	249.0
27	306.0
28	306.0
29	306.0
30	467.0
31	628.0
32	628.0
33	628.0
34	991.5
35	1355.0
36	1355.0
37	1355.0
38	1945.5
39	2536.0
40	2536.0
41	2536.0
42	3405.5
43	4275.0
44	4275.0
45	4275.0
46	4901.5
47	5528.0
48	5528.0
49	5528.0
50	6188.0
51	6848.0
52	6848.0
53	6848.0
54	6555.0
55	6262.0
56	6262.0
57	6262.0
58	5512.0
59	4762.0
60	4762.0
61	4762.0
62	3819.0
63	2876.0
64	2876.0
65	2876.0
66	2258.0
67	1640.0
68	1640.0
69	1640.0
70	1239.5
71	839.0
72	839.0
73	839.0
74	583.5
75	328.0
76	328.0
77	328.0
78	248.5
79	169.0
80	169.0
81	169.0
82	119.0
83	69.0
84	69.0
85	69.0
86	42.0
87	15.0
88	15.0
89	15.0
90	10.0
91	5.0
92	5.0
93	5.0
94	3.5
95	2.0
96	2.0
97	2.0
98	7.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0025763899623847068
4	0.0051527799247694135
5	0.02318750966146236
6	0.061833359097232955
7	0.09275003864584944
8	0.09017364868346474
9	0.10563198845777297
10	0.12109032823208121
11	0.1262431081568506
12	0.1185139382696965
13	0.1185139382696965
14	0.10305559849538826
15	0.12881949811923535
16	0.10047920853300356
17	0.1185139382696965
18	0.10047920853300356
19	0.08244447879631062
20	0.0747153089091565
21	0.08502086875869531
22	0.10047920853300356
23	0.06956252898438707
24	0.0798680888339259
25	0.0747153089091565
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	38814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.15489256453857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.73915169294707	26.869170917710107
2	13.348001466813347	11.2536713556964
3	6.790123456790123	8.587107744628227
4	4.272093876054272	7.20358633482764
5	2.786945361202787	5.874169114237131
6	2.1635496883021634	5.472252280105117
7	1.442366458868109	4.256196217859536
8	1.0634396773010635	3.586334827639512
9	0.8556411196675223	3.2462513526047303
>10	3.502016868353502	22.558870510640492
>50	0.030558611416697225	0.8218683980007213
>100	0.006111722283339446	0.2705209460503942
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	105	0.2705209460503942	No Hit
ATATAATCCGCGCCTTTCGTCTCGG	81	0.20868758695316122	No Hit
TATCAACGCAGAGTACTTTTTTTTT	73	0.18807646725408358	No Hit
TCATACAACTGCTCCGCATTCTGCT	61	0.1571597877054671	No Hit
CTCTAGGCCTGATTGAAATTCTCTT	53	0.13654866800638946	No Hit
GTATCAACGCAGAGTACATGGGATC	51	0.13139588808162003	No Hit
GCGTATGCAAGAGCCTTGATGAGGA	48	0.12366671819446591	No Hit
GTCGTAGGTAGCGCAGGCGACGTGG	43	0.11078476838254236	No Hit
ATTATACACCTTGGCTTGCAAACCA	43	0.11078476838254236	No Hit
GGTATCAACGCAGAGTACTTTTTTT	43	0.11078476838254236	No Hit
TGCATACGCTGCTCGCAAGGTTCCA	42	0.10820837842015767	No Hit
ATTGTACATCAACTTCACACCGGAG	42	0.10820837842015767	No Hit
GTATAATACTGCTACTGCCTCTGAG	41	0.10563198845777297	No Hit
GTCTACAACATCCCCAAAATTCGAC	40	0.10305559849538826	No Hit
ACCTACGACTGCTCTTCCAGCGGTC	40	0.10305559849538826	No Hit
TACCAGTACTGTACAGAATGGGGCT	39	0.10047920853300356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE