FastQCFastQC Report
Thu 2 Feb 2017
SRR4061968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38814
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAGTACTGTACAGAATGGGGCTACCTCCAAACTGGCAATGTTCCAGA520.13397227804400474No Hit
GTACTGCACCAGTGAGGCAAGAATCGCCGCATTCATTGATGCCATGAGAC500.12881949811923535No Hit
TCATACAACTGCTCCGCATTCTGCTTCGCATGACGCTTGGCCTTTTCACG490.1262431081568506No Hit
GTATAGGCTTGATGAACAGAGACTCCTCTAAGCGAGCTGCCAAAGTACTT490.1262431081568506No Hit
ATATAATCCGCGCCTTTCGTCTCGGCGAGACGGTCGACTTCCACGCCGGC490.1262431081568506No Hit
TGCATACGCTGCTCGCAAGGTTCCAGCCGCCAACAGCTCATGGCGTGAGG430.11078476838254236No Hit
CACTATACATTCGCCAAAAAGCCCAGACCTATAATTTCAAGCCCATTCTT400.10305559849538826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGCC300.005677622729.33333218
GTATCAA1053.6379788E-1227.2380961
GATGTTG450.001374002424.44444528
TATCAAC1556.511982E-1018.4516122
CATGGGG750.001260595317.616
ATCAACG1451.1602242E-716.6896553
AGAGTAC1501.7071761E-716.13333311
AACGCAG1552.4786823E-715.6129036
TCAACGC1552.4786823E-715.6129034
CGCAGAG1552.4786823E-715.6129038
CAACGCA1552.4786823E-715.6129035
CAGAGTA1603.554178E-715.12510
ACGCAGA1655.0369454E-714.6666677
AGTACAT1150.001381004113.39130313
GCAGAGT2004.3869422E-612.0999999
GAGTACA1300.003469434811.84615412
GTACATG1400.006032262511.014
Produced by FastQC (version 0.11.5)