##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38814
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.048925645385687	32.0	32.0	32.0	32.0	32.0
2	30.922527953831093	32.0	32.0	32.0	32.0	32.0
3	31.27618900396764	32.0	32.0	32.0	32.0	32.0
4	31.452207966197765	32.0	32.0	32.0	32.0	32.0
5	31.284716854743134	32.0	32.0	32.0	32.0	32.0
6	34.97632297624568	36.0	36.0	36.0	36.0	36.0
7	34.94772504766321	36.0	36.0	36.0	36.0	36.0
8	34.89413613644561	36.0	36.0	36.0	32.0	36.0
9	35.0586643994435	36.0	36.0	36.0	36.0	36.0
10	34.818931313443606	36.0	36.0	36.0	32.0	36.0
11	35.05134745195033	36.0	36.0	36.0	36.0	36.0
12	34.96727984747771	36.0	36.0	36.0	32.0	36.0
13	35.03604369557376	36.0	36.0	36.0	36.0	36.0
14	34.91549440923378	36.0	36.0	36.0	32.0	36.0
15	34.88926675941671	36.0	36.0	36.0	32.0	36.0
16	34.92237337043335	36.0	36.0	36.0	32.0	36.0
17	34.92386767661153	36.0	36.0	36.0	32.0	36.0
18	34.92543927448859	36.0	36.0	36.0	32.0	36.0
19	34.905137321584995	36.0	36.0	36.0	32.0	36.0
20	34.86090070593085	36.0	36.0	36.0	32.0	36.0
21	34.90943989282218	36.0	36.0	36.0	32.0	36.0
22	34.87406605863863	36.0	36.0	36.0	32.0	36.0
23	34.809295614984286	36.0	36.0	36.0	32.0	36.0
24	34.822460967692066	36.0	36.0	36.0	32.0	36.0
25	34.80805894780234	36.0	36.0	36.0	32.0	36.0
26	34.728963775957126	36.0	36.0	36.0	32.0	36.0
27	34.72399134332973	36.0	36.0	36.0	32.0	36.0
28	34.71376307517906	36.0	36.0	36.0	32.0	36.0
29	34.687818828257846	36.0	36.0	36.0	32.0	36.0
30	34.68274334003195	36.0	36.0	36.0	32.0	36.0
31	34.69024063482249	36.0	36.0	36.0	32.0	36.0
32	34.6425001288195	36.0	36.0	36.0	32.0	36.0
33	34.6099860874942	36.0	36.0	36.0	32.0	36.0
34	34.60617303034987	36.0	36.0	36.0	32.0	36.0
35	34.535373834183545	36.0	36.0	36.0	32.0	36.0
36	34.52823723398774	36.0	36.0	36.0	32.0	36.0
37	34.5297057762663	36.0	36.0	36.0	32.0	36.0
38	34.53650744576699	36.0	36.0	36.0	32.0	36.0
39	34.54351522646468	36.0	36.0	36.0	32.0	36.0
40	34.51486577008296	36.0	36.0	36.0	32.0	36.0
41	34.457103107126294	36.0	36.0	36.0	32.0	36.0
42	34.446874838975624	36.0	36.0	36.0	32.0	36.0
43	34.42149739784614	36.0	36.0	36.0	32.0	36.0
44	34.41791621579842	36.0	36.0	36.0	32.0	36.0
45	34.366980986242076	36.0	36.0	36.0	32.0	36.0
46	34.33304993043747	36.0	36.0	36.0	32.0	36.0
47	34.3009996393054	36.0	36.0	36.0	32.0	36.0
48	34.309501726181274	36.0	36.0	36.0	32.0	36.0
49	34.18423764621013	36.0	36.0	36.0	32.0	36.0
50	33.585278507754936	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	3.0
24	25.0
25	40.0
26	81.0
27	197.0
28	336.0
29	578.0
30	997.0
31	1453.0
32	2426.0
33	4112.0
34	9153.0
35	19410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.553565445970676	19.33871092441306	12.475839496946113	27.631884132670155
2	16.49704995748847	20.248370391363718	36.675856027619616	26.578723623528198
3	18.61699386819189	24.596794970886794	26.222497037151545	30.563714123769774
4	14.773020044313908	15.594888442314629	32.87215953006647	36.759931983304995
5	14.80136033390014	36.35801514917298	31.256763023651256	17.58386149327562
6	32.97006234863709	33.67084041840573	15.947853867161331	17.411243365795848
7	30.081929200803835	28.68294945122894	20.255577884268565	20.979543463698665
8	26.315247075797394	32.72272891224816	18.9519245633019	22.010099448652547
9	26.245684546813003	13.70639459988664	17.674035141959088	42.373885711341266
10	18.39284794146442	25.230586901633433	28.94831761735456	27.428247539547584
11	36.37604987890967	21.494821456175607	20.683258618024425	21.4458700468903
12	23.1643221518009	22.43262740248364	26.735198639666102	27.667851806049367
13	29.901581903436902	18.921007883753287	23.682176534240224	27.495233678569587
14	22.759828927706497	18.565466068944193	22.589787190189107	36.0849178131602
15	27.131962693873348	25.68145514505075	19.923223579120936	27.263358581954968
16	25.84892049260576	26.87174730767249	20.804348946256507	26.474983253465247
17	23.483794507136597	24.426753233369404	24.7642603184418	27.325191941052196
18	24.1104784479427	24.50982918094453	23.517893489294824	27.861798881817947
19	25.599010666254447	24.29793373525017	24.2077600865667	25.895295511928683
20	26.266295666512086	23.558509816045756	22.754676147781726	27.420518369660428
21	26.96449734631834	23.048384603493584	22.844849796465194	27.142268253722886
22	25.88756634204153	22.801051167104653	23.360127788942133	27.951254701911683
23	25.799969083320452	23.36785695882929	23.682176534240224	27.14999742361004
24	24.076364198485084	24.62255887051064	22.60266914000103	28.698407791003245
25	25.805121863245223	23.700211263976918	22.978822074509196	27.515844798268667
26	24.862803699791307	24.816427485636254	23.358153196094094	26.962615618478345
27	25.686607924975526	22.76755809759365	23.924357190704384	27.62147678672644
28	25.548799340410184	22.84602700195816	23.63186643306194	27.973307224569723
29	24.828670067501417	23.95012109032823	23.568815375895298	27.652393466275054
30	25.4547328283609	23.517287576647604	23.403926418302675	27.62405317668882
31	24.990982635131655	24.303086515174936	24.15623228731901	26.549698562374402
32	24.975524295357346	23.512134796722833	24.57360746122533	26.938733446694492
33	26.194156747565312	23.39104446849075	22.370794043386407	28.044004740557533
34	25.34394805997836	24.1072808780337	23.42453753800175	27.12423352398619
35	25.006440974905964	23.81872520224661	23.97073221002731	27.204101612820114
36	24.908538156335343	23.115370742515587	22.888648425825732	29.087442675323338
37	25.725253774411293	24.27216983562632	23.921780800742003	26.080795589220386
38	25.72010099448653	22.87061369608904	23.450301437625598	27.958983871798836
39	26.27402483639924	23.52244035657237	23.076724893079817	27.126809913948573
40	25.712371824599373	24.47055186272994	22.78816921729273	27.028907095377956
41	26.858865357860566	24.20518369660432	22.424898232596487	26.511052712938632
42	24.771989488328952	23.0793012830422	23.409079198227445	28.739630030401404
43	26.098830318957077	23.77492657288607	22.855155356314732	27.27108775184212
44	25.671149585201213	23.318905549543977	23.847065491832844	27.162879373421962
45	25.650538465502137	23.7362807234503	24.279899005513474	26.333281805534085
46	26.565156902148708	23.277683310145825	23.45545421755037	26.7017055701551
47	25.986138664880325	22.06219565609461	23.571999072475716	28.379666606549353
48	25.565517596743444	23.75173906322461	23.10764157262843	27.575101767403513
49	27.013448755603648	23.1643221518009	22.597516360076263	27.224712732519198
50	25.84443356607322	23.01033158993121	23.914667765954707	27.230567078040863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	3.0
4	3.0
5	5.0
6	7.0
7	5.0
8	3.0
9	5.5
10	8.0
11	10.0
12	12.0
13	33.5
14	55.0
15	65.0
16	75.0
17	76.0
18	77.0
19	60.5
20	44.0
21	45.0
22	46.0
23	37.5
24	29.0
25	40.0
26	51.0
27	51.5
28	52.0
29	53.0
30	54.0
31	77.0
32	100.0
33	121.0
34	142.0
35	207.5
36	273.0
37	354.0
38	435.0
39	575.5
40	716.0
41	900.0
42	1084.0
43	1471.5
44	1859.0
45	2423.5
46	2988.0
47	3261.0
48	3534.0
49	3591.5
50	3649.0
51	4084.0
52	4519.0
53	4469.0
54	4419.0
55	4117.5
56	3816.0
57	3329.5
58	2843.0
59	2675.0
60	2507.0
61	2098.5
62	1690.0
63	1443.0
64	1196.0
65	1020.0
66	844.0
67	706.0
68	568.0
69	510.0
70	452.0
71	360.5
72	269.0
73	214.0
74	159.0
75	133.0
76	107.0
77	84.5
78	62.0
79	44.0
80	26.0
81	24.0
82	22.0
83	14.5
84	7.0
85	5.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028340289586231773
2	0.0025763899623847068
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0025763899623847068
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0025763899623847068
27	0.0
28	0.0051527799247694135
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0025763899623847068
48	0.0
49	0.0
50	0.0025763899623847068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	38814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.3396712526408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.79895409645556	29.175039934044417
2	12.678675188843696	11.24336579584686
3	6.6240557815223715	8.811253671355697
4	3.933759442184776	6.9768640181377855
5	2.6380011621150494	5.848405214613283
6	1.8303312027890761	4.8693770289070955
7	1.47588611272516	4.580821353120008
8	1.063335270191749	3.77183490493121
9	0.8192911098198722	3.2694388622661927
>10	3.131900058105753	21.319626938733446
>50	0.005810575246949448	0.13397227804400474
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCAGTACTGTACAGAATGGGGCTACCTCCAAACTGGCAATGTTCCAGA	52	0.13397227804400474	No Hit
GTACTGCACCAGTGAGGCAAGAATCGCCGCATTCATTGATGCCATGAGAC	50	0.12881949811923535	No Hit
TCATACAACTGCTCCGCATTCTGCTTCGCATGACGCTTGGCCTTTTCACG	49	0.1262431081568506	No Hit
GTATAGGCTTGATGAACAGAGACTCCTCTAAGCGAGCTGCCAAAGTACTT	49	0.1262431081568506	No Hit
ATATAATCCGCGCCTTTCGTCTCGGCGAGACGGTCGACTTCCACGCCGGC	49	0.1262431081568506	No Hit
TGCATACGCTGCTCGCAAGGTTCCAGCCGCCAACAGCTCATGGCGTGAGG	43	0.11078476838254236	No Hit
CACTATACATTCGCCAAAAAGCCCAGACCTATAATTTCAAGCCCATTCTT	40	0.10305559849538826	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0051527799247694135	0.0
30	0.0	0.0	0.0	0.0051527799247694135	0.0
31	0.0	0.0	0.0	0.0051527799247694135	0.0
32	0.0	0.0	0.0	0.0051527799247694135	0.0
33	0.0	0.0	0.0	0.018034729736692946	0.0
34	0.0	0.0	0.0	0.025763899623847064	0.0
35	0.0	0.0	0.0	0.030916679548616478	0.0
36	0.0	0.0	0.0	0.04122223939815531	0.0
37	0.0	0.0	0.0	0.05152779924769413	0.0
38	0.0	0.0	0.0	0.08244447879631062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGAGCC	30	0.0056776227	29.333332	18
GTATCAA	105	3.6379788E-12	27.238096	1
GATGTTG	45	0.0013740024	24.444445	28
TATCAAC	155	6.511982E-10	18.451612	2
CATGGGG	75	0.0012605953	17.6	16
ATCAACG	145	1.1602242E-7	16.689655	3
AGAGTAC	150	1.7071761E-7	16.133333	11
AACGCAG	155	2.4786823E-7	15.612903	6
TCAACGC	155	2.4786823E-7	15.612903	4
CGCAGAG	155	2.4786823E-7	15.612903	8
CAACGCA	155	2.4786823E-7	15.612903	5
CAGAGTA	160	3.554178E-7	15.125	10
ACGCAGA	165	5.0369454E-7	14.666667	7
AGTACAT	115	0.0013810041	13.391303	13
GCAGAGT	200	4.3869422E-6	12.099999	9
GAGTACA	130	0.0034694348	11.846154	12
GTACATG	140	0.0060322625	11.0	14
>>END_MODULE