FastQCFastQC Report
Thu 2 Feb 2017
SRR4061967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67592
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3790.5607172446443366No Hit
ATTCCATTCCATTCCATTCCATTCC2590.38318144159072076No Hit
GAATGGAATGGAATGGAATGGAATG2240.33140016570008285No Hit
TATCAACGCAGAGTACTTTTTTTTT1980.2929340750384661No Hit
GGTATCAACGCAGAGTACTTTTTTT1390.20564563853710496No Hit
CTAATAAACAGCATGATGAAGAAAC1290.190850988282637No Hit
ATACTAGAGTCCGCAAAAAGGACCT1080.15978222274825424No Hit
GGGCTACAGCGGCGGACATGAGCAG1050.15534382767191385No Hit
GTACTGGTCGTAGCGGTCGACGAAC1040.15386436264646705No Hit
GTATCAACGCAGAGTACATGGGGAG1030.15238489762102025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT970.14350810746833945No Hit
ATGCTACCCTCACCTTTCGGGCTGG940.13906971239199906No Hit
GTATCAACGCAGAGTACATGGGAGG930.13759024736655226No Hit
TATTTAGCCTTGGACGGAATTTACC900.13315185229021187No Hit
GATCAAGGCAGTTCTGAGAGCGGGA890.13167238726476507No Hit
CCTCATAACCACCGCAAACCGCCAA860.12723399218842465No Hit
GAATGGAATGGAATGCAATGGAATG850.12575452716297786No Hit
TCTCAGCTCCGTTTGCTTTTGAGGT830.12279559711208428No Hit
GCCCATGACCCTGCTGCTCGTAGCC800.11835720203574387No Hit
GTCCTGGTCCTGACCAGAGAGGTCG770.11391880695940347No Hit
ATGCTAAGGGTTGCAGATGTAACGG770.11391880695940347No Hit
ATGTTAGCATGATGGCAGATTCAAC740.1094804118830631No Hit
GGCTACGAGCAGCAAGGGCATGGCG730.1080009468576163No Hit
GTACATGGGGGCATGAGCTCTTTTT730.1080009468576163No Hit
GTCTCATATCGCGCTTAAAATGGGT720.10652148183216947No Hit
GTACATGGGGAGCTGTGAATGAGTT710.10504201680672269No Hit
GGGTAGCATCGTGCACTGGACTTTG700.1035625517812759No Hit
GTCATGGGCCAGGAGGCTATCAGCA690.1020830867558291No Hit
GCCCTAAGATTGTCGTCCTCGAGCA680.1006036217303823No Hit
CCATATATCCAATGTCAGCCCTGGC680.1006036217303823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA855.2750693E-1116.7647061
GTATCAA1803.5288394E-1010.0277781
CAACGCA2302.8991053E-87.84782655
TATCAAC2406.1547325E-87.52083352
TCAACGC2552.2380846E-87.4509814
ATCAACG2552.2380846E-87.4509813
AACGCAG2458.850293E-87.36734726
CGCAGAG2551.3563367E-66.7058838
ACGCAGA2601.8676783E-66.5769237
CAGAGTA2601.8676783E-66.57692310
GCAGAGT2601.8676783E-66.5769239
AGAGTAC2652.5537956E-66.4528311
CATGGGG1900.00325894176.04
AGTACAT2150.00202617335.744186413
GAGTACA2359.3802385E-45.65957412
ACATGGG3103.2348977E-55.5161293
GTACATG2959.120462E-55.47457651
TACATGG2959.120462E-55.47457652
Produced by FastQC (version 0.11.5)