##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061967_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67592
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24344596993727	32.0	32.0	32.0	32.0	32.0
2	31.00665759261451	32.0	32.0	32.0	32.0	32.0
3	30.998165463368444	32.0	32.0	32.0	32.0	32.0
4	31.00397976091845	32.0	32.0	32.0	32.0	32.0
5	31.024884601728015	32.0	32.0	32.0	32.0	32.0
6	34.633714048999884	36.0	36.0	36.0	32.0	36.0
7	34.60314830157415	36.0	36.0	36.0	32.0	36.0
8	34.541114333057166	36.0	36.0	36.0	32.0	36.0
9	34.546973014557935	36.0	36.0	36.0	32.0	36.0
10	34.405240265120135	36.0	36.0	36.0	32.0	36.0
11	34.56150136110782	36.0	36.0	36.0	32.0	36.0
12	34.49758847200852	36.0	36.0	36.0	32.0	36.0
13	34.51266422061782	36.0	36.0	36.0	32.0	36.0
14	34.436397798556044	36.0	36.0	36.0	32.0	36.0
15	34.41818558409279	36.0	36.0	36.0	32.0	36.0
16	34.38815244407622	36.0	36.0	36.0	32.0	36.0
17	34.373831222629896	36.0	36.0	36.0	32.0	36.0
18	34.34061723280862	36.0	36.0	36.0	32.0	36.0
19	34.295271629778675	36.0	36.0	36.0	32.0	36.0
20	34.26868564327139	36.0	36.0	36.0	32.0	36.0
21	34.253373180258016	36.0	36.0	36.0	32.0	36.0
22	34.28818499230678	36.0	36.0	36.0	32.0	36.0
23	34.20270150313647	36.0	36.0	36.0	32.0	36.0
24	34.148331163451296	36.0	36.0	36.0	32.0	36.0
25	33.80139661498402	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	6.0
6	7.0
7	5.0
8	14.0
9	11.0
10	2.0
11	3.0
12	3.0
13	0.0
14	48.0
15	70.0
16	77.0
17	86.0
18	99.0
19	124.0
20	155.0
21	173.0
22	232.0
23	239.0
24	343.0
25	451.0
26	635.0
27	738.0
28	973.0
29	1246.0
30	1795.0
31	2390.0
32	3607.0
33	5378.0
34	13223.0
35	35458.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.573715435486974	20.850409078131705	12.636297731946561	23.93957775443476
2	16.26671795478755	21.44632500887679	36.93484436027932	25.352112676056336
3	17.70771688957273	27.13190910166884	27.63048881524441	27.529885193514026
4	12.640939895238375	15.641738924565713	34.14666627208428	37.57065490811163
5	16.39167172262752	34.50878257395268	30.553294760051497	18.546250943368307
6	31.84610602045801	36.24857519281157	16.376770831791333	15.528547954939084
7	27.98009567110466	30.58661493120863	21.912533507101283	19.52075589058543
8	25.603921975206244	33.6181998607758	20.023105292000533	20.75477287201742
9	26.12727380458612	13.676897552882622	20.13539136102388	40.060437281507376
10	17.838254248085214	25.501844417119745	27.19515266440497	29.46474867039007
11	35.48640438615989	24.96110246721494	20.0	19.552493146625178
12	23.219153727628303	25.29038757852317	27.932025601517125	23.558433092331395
13	27.698438564783267	23.217089864004027	24.40964712156677	24.674824449645936
14	22.456748429908757	17.7568432278706	25.25032586799384	34.5360824742268
15	26.69037037037037	25.340740740740742	21.282962962962962	26.685925925925925
16	26.39024679287767	28.34562853672266	20.590762302610138	24.67336236778953
17	22.458593819442388	27.051050339249205	26.224408165683982	24.265947675624425
18	23.144033653276455	28.168325630998936	24.563040644626142	24.12460007109847
19	24.87966706654226	23.271278565187124	26.10891426371055	25.740140104560062
20	27.174186669825712	23.37000784824747	23.321141401726614	26.134664080200203
21	27.973581329226395	25.081447695770642	23.28811752162066	23.6568534533823
22	25.76971492039985	25.00999629766753	25.919289152165863	23.300999629766753
23	23.603358159231234	26.9704014095977	25.349067918326252	24.07717251284481
24	23.862693993602655	23.142992536429333	26.264660585238715	26.729652884729298
25	26.083930581057867	24.317360658650713	22.79808090979091	26.800627850500504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	92.0
2	67.5
3	43.0
4	43.0
5	43.0
6	84.0
7	125.0
8	125.0
9	125.0
10	135.0
11	145.0
12	145.0
13	145.0
14	139.5
15	134.0
16	134.0
17	134.0
18	185.5
19	237.0
20	237.0
21	237.0
22	388.0
23	539.0
24	539.0
25	539.0
26	895.0
27	1251.0
28	1251.0
29	1251.0
30	2005.5
31	2760.0
32	2760.0
33	2760.0
34	4026.0
35	5292.0
36	5292.0
37	5292.0
38	6190.0
39	7088.0
40	7088.0
41	7088.0
42	7383.5
43	7679.0
44	7679.0
45	7679.0
46	8084.0
47	8489.0
48	8489.0
49	8489.0
50	8388.0
51	8287.0
52	8287.0
53	8287.0
54	7705.0
55	7123.0
56	7123.0
57	7123.0
58	6561.5
59	6000.0
60	6000.0
61	6000.0
62	5444.0
63	4888.0
64	4888.0
65	4888.0
66	4302.0
67	3716.0
68	3716.0
69	3716.0
70	2769.5
71	1823.0
72	1823.0
73	1823.0
74	1463.5
75	1104.0
76	1104.0
77	1104.0
78	810.5
79	517.0
80	517.0
81	517.0
82	340.5
83	164.0
84	164.0
85	164.0
86	112.5
87	61.0
88	61.0
89	61.0
90	37.5
91	14.0
92	14.0
93	14.0
94	10.0
95	6.0
96	6.0
97	6.0
98	10.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014794650254467984
2	0.0
3	0.0
4	0.014794650254467984
5	0.022191975381701978
6	0.05769913599242514
7	0.1020830867558291
8	0.11095987690850988
9	0.12427506213753108
10	0.13463131731565867
11	0.1583027577228074
12	0.14202864244289265
13	0.13315185229021187
14	0.11835720203574387
15	0.13611078234110544
16	0.12723399218842465
17	0.13315185229021187
18	0.11835720203574387
19	0.10504201680672269
20	0.0902473665522547
21	0.0946857616285951
22	0.09912415670493549
23	0.08137057639957392
24	0.0946857616285951
25	0.08876790152680791
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	67592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.137708604568587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.07437595517065	15.179311161084152
2	11.643176543838795	6.086519114688129
3	6.464028980585272	5.068647177180732
4	4.499915095941586	4.704698780920819
5	3.164091243561442	4.135104746123801
6	2.1792041659591326	3.417564208782104
7	1.8961906379124922	3.4693454846727425
8	1.5112922397690611	3.1601372943543615
9	1.2226184411614875	2.876080009468576
>10	8.886624780664516	42.633743638300395
>50	0.4018792098262297	6.682743519943189
>100	0.056602705609328126	2.586104864481004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	379	0.5607172446443366	No Hit
ATTCCATTCCATTCCATTCCATTCC	259	0.38318144159072076	No Hit
GAATGGAATGGAATGGAATGGAATG	224	0.33140016570008285	No Hit
TATCAACGCAGAGTACTTTTTTTTT	198	0.2929340750384661	No Hit
GGTATCAACGCAGAGTACTTTTTTT	139	0.20564563853710496	No Hit
CTAATAAACAGCATGATGAAGAAAC	129	0.190850988282637	No Hit
ATACTAGAGTCCGCAAAAAGGACCT	108	0.15978222274825424	No Hit
GGGCTACAGCGGCGGACATGAGCAG	105	0.15534382767191385	No Hit
GTACTGGTCGTAGCGGTCGACGAAC	104	0.15386436264646705	No Hit
GTATCAACGCAGAGTACATGGGGAG	103	0.15238489762102025	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	97	0.14350810746833945	No Hit
ATGCTACCCTCACCTTTCGGGCTGG	94	0.13906971239199906	No Hit
GTATCAACGCAGAGTACATGGGAGG	93	0.13759024736655226	No Hit
TATTTAGCCTTGGACGGAATTTACC	90	0.13315185229021187	No Hit
GATCAAGGCAGTTCTGAGAGCGGGA	89	0.13167238726476507	No Hit
CCTCATAACCACCGCAAACCGCCAA	86	0.12723399218842465	No Hit
GAATGGAATGGAATGCAATGGAATG	85	0.12575452716297786	No Hit
TCTCAGCTCCGTTTGCTTTTGAGGT	83	0.12279559711208428	No Hit
GCCCATGACCCTGCTGCTCGTAGCC	80	0.11835720203574387	No Hit
GTCCTGGTCCTGACCAGAGAGGTCG	77	0.11391880695940347	No Hit
ATGCTAAGGGTTGCAGATGTAACGG	77	0.11391880695940347	No Hit
ATGTTAGCATGATGGCAGATTCAAC	74	0.1094804118830631	No Hit
GGCTACGAGCAGCAAGGGCATGGCG	73	0.1080009468576163	No Hit
GTACATGGGGGCATGAGCTCTTTTT	73	0.1080009468576163	No Hit
GTCTCATATCGCGCTTAAAATGGGT	72	0.10652148183216947	No Hit
GTACATGGGGAGCTGTGAATGAGTT	71	0.10504201680672269	No Hit
GGGTAGCATCGTGCACTGGACTTTG	70	0.1035625517812759	No Hit
GTCATGGGCCAGGAGGCTATCAGCA	69	0.1020830867558291	No Hit
GCCCTAAGATTGTCGTCCTCGAGCA	68	0.1006036217303823	No Hit
CCATATATCCAATGTCAGCCCTGGC	68	0.1006036217303823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0014794650254467984	0.0
6	0.0	0.0	0.0	0.0014794650254467984	0.0
7	0.0	0.0	0.0	0.0014794650254467984	0.0
8	0.0	0.0	0.0	0.0014794650254467984	0.0
9	0.0	0.0	0.0	0.0014794650254467984	0.0
10	0.0	0.0	0.0	0.0014794650254467984	0.0
11	0.0	0.0	0.0	0.0014794650254467984	0.0
12	0.0	0.0	0.0	0.0014794650254467984	0.0
13	0.0	0.0	0.0	0.0014794650254467984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	85	5.2750693E-11	16.764706	1
GTATCAA	180	3.5288394E-10	10.027778	1
CAACGCA	230	2.8991053E-8	7.8478265	5
TATCAAC	240	6.1547325E-8	7.5208335	2
TCAACGC	255	2.2380846E-8	7.450981	4
ATCAACG	255	2.2380846E-8	7.450981	3
AACGCAG	245	8.850293E-8	7.3673472	6
CGCAGAG	255	1.3563367E-6	6.705883	8
ACGCAGA	260	1.8676783E-6	6.576923	7
CAGAGTA	260	1.8676783E-6	6.576923	10
GCAGAGT	260	1.8676783E-6	6.576923	9
AGAGTAC	265	2.5537956E-6	6.45283	11
CATGGGG	190	0.0032589417	6.0	4
AGTACAT	215	0.0020261733	5.7441864	13
GAGTACA	235	9.3802385E-4	5.659574	12
ACATGGG	310	3.2348977E-5	5.516129	3
GTACATG	295	9.120462E-5	5.4745765	1
TACATGG	295	9.120462E-5	5.4745765	2
>>END_MODULE