FastQCFastQC Report
Thu 2 Feb 2017
SRR4061967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67592
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.3106876553438277No Hit
CTAATAAACAGCATGATGAAGAAACGTTTCGCATCGAGAAAAATTGATCC1230.18197419812995622No Hit
ATACTAGAGTCCGCAAAAAGGACCTAATAATCTCATTTGGTTCCCTCGTA1010.14942596757012663No Hit
GTACTGGTCGTAGCGGTCGACGAACTGGTCCTCGAGCTCACCGATCTCGT1010.14942596757012663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.14794650254467984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.14202864244289265No Hit
ATGCTACCCTCACCTTTCGGGCTGGCTTGAATGATGCCCTTGAAGCTCTT930.13759024736655226No Hit
CTATAACCCTGCTGATACCCGCTACTTTGCCCGTACCCGGGAGCGTCGTT860.12723399218842465No Hit
ATCCTGGATGATGGCCTTGGAAGCCTCGTATCCGTCGTAGGCGTTGCGGG840.12427506213753108No Hit
GTCCTGGTCCTGACCAGAGAGGTCGTGGCCAGATGTTTGGTTGCGACGGC840.12427506213753108No Hit
GATCAAGGCAGTTCTGAGAGCGGGAGTGCTTGCCAGGCGGCATGTCTCAG830.12279559711208428No Hit
CCTCATAACCACCGCAAACCGCCAAAATGCTCGCCGCCCGCACCGCCACC820.12131613208663747No Hit
CACATACTCCATGCGCCAGTCGCGCGCGCCGACTGCCTCGCAGATCCAGA810.11983666706119067No Hit
GAATAAGCTAAGCCACCCTTCATTGATCAGGCTTTTCATTAATCTAGAAA810.11983666706119067No Hit
GTCTCATATCGCGCTTAAAATGGGTTGTCCTCTGGAGCAACCTCCTTGTT800.11835720203574387No Hit
ACCAAGCACTGTACGACTCTCTGCACGCCAAAGACCACACCTCATAACCA790.11687773701029708No Hit
GGGCTACAGCGGCGGACATGAGCAGCAGGTTTACGGAGCAGGAGGCTACC760.11243934193395667No Hit
CACATACACAGCAAAATACCTATCACACCGCAATCATGGACATGATCGGC740.1094804118830631No Hit
GTACATGGGGAGCTGTGAATGAGTTGGTTGTTCGAGAGGGAAGGCATTTT730.1080009468576163No Hit
GTGAATGAGTTGGTTGTTCGAGAGGGAAGGCATTTTCTTATAATGGCGTT720.10652148183216947No Hit
CTCTAAGACAAGAGAACCAACCCAATGCAAAACGCCATATGCTCGCAGAA700.1035625517812759No Hit
TTTTTATAGTGGGTGTTTCAGCTCCATCCGCGAGGGACCTCACCTACATA690.1020830867558291No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT690.1020830867558291No Hit
GGGTAGCATCGTGCACTGGACTTTGGAGTATGAAAAGTTGCACAACCAAA690.1020830867558291No Hit
CTCCCAGAGCGCCCATGCTCCCCAGTAAAACTCCGAAATGCTCCCTCTAA680.1006036217303823No Hit
ATCATGGACATGATCGGCAAGGTCGCTAAATCGCTGCTCGACAAGAACGG680.1006036217303823No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA680.1006036217303823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTCG207.803912E-444.035
CGGGAAA207.803912E-444.040
ACCTCGG207.803912E-444.036
GGGAAAA207.803912E-444.041
AAGCATA207.803912E-444.033
TCAAGGC357.1889644E-637.7142873
GCAGTTC357.1889644E-637.7142878
CAAGGCA357.1889644E-637.7142874
ATCAAGG357.1889644E-637.7142872
GTGCTTG357.1889644E-637.71428726
TGAGAGC357.1889644E-637.71428715
GCGGGAG301.2881715E-436.66666420
GGGAGTG301.2881715E-436.66666422
AGCGGGA301.2881715E-436.66666419
CGGGAGT301.2881715E-436.66666421
GAAAAGG250.002337152435.243
AATGACA250.002337152435.226
GTCTCAG401.79489E-533.044
AAGGCAG401.79489E-533.05
GCTTGCC401.79489E-533.028