##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67592
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191102497336963	32.0	32.0	32.0	32.0	32.0
2	31.514750266303704	32.0	32.0	32.0	32.0	32.0
3	31.625059178601017	32.0	32.0	32.0	32.0	32.0
4	31.717333412238133	32.0	32.0	32.0	32.0	32.0
5	31.66923600426086	32.0	32.0	32.0	32.0	32.0
6	35.31978636525032	36.0	36.0	36.0	36.0	36.0
7	35.34344301100722	36.0	36.0	36.0	36.0	36.0
8	35.29197242277193	36.0	36.0	36.0	36.0	36.0
9	35.33261332702095	36.0	36.0	36.0	36.0	36.0
10	35.26256065806604	36.0	36.0	36.0	36.0	36.0
11	35.369318854302286	36.0	36.0	36.0	36.0	36.0
12	35.31340987099065	36.0	36.0	36.0	36.0	36.0
13	35.31172328086164	36.0	36.0	36.0	36.0	36.0
14	35.27270978814061	36.0	36.0	36.0	36.0	36.0
15	35.23150668718191	36.0	36.0	36.0	36.0	36.0
16	35.25420168067227	36.0	36.0	36.0	36.0	36.0
17	35.24491064031246	36.0	36.0	36.0	36.0	36.0
18	35.22745295301219	36.0	36.0	36.0	36.0	36.0
19	35.168229968043555	36.0	36.0	36.0	36.0	36.0
20	35.1718546573559	36.0	36.0	36.0	36.0	36.0
21	35.14165877618653	36.0	36.0	36.0	36.0	36.0
22	35.1533021659368	36.0	36.0	36.0	36.0	36.0
23	35.100707184282165	36.0	36.0	36.0	36.0	36.0
24	35.03358385607764	36.0	36.0	36.0	36.0	36.0
25	35.02405610131377	36.0	36.0	36.0	36.0	36.0
26	34.94872174221801	36.0	36.0	36.0	36.0	36.0
27	34.98946620901882	36.0	36.0	36.0	36.0	36.0
28	34.92979938454255	36.0	36.0	36.0	36.0	36.0
29	34.83595691797846	36.0	36.0	36.0	32.0	36.0
30	34.796839862705646	36.0	36.0	36.0	32.0	36.0
31	34.79820097052906	36.0	36.0	36.0	32.0	36.0
32	34.80110072197893	36.0	36.0	36.0	32.0	36.0
33	34.77091963545982	36.0	36.0	36.0	32.0	36.0
34	34.66738667297905	36.0	36.0	36.0	32.0	36.0
35	34.65816960587052	36.0	36.0	36.0	32.0	36.0
36	34.58421114924843	36.0	36.0	36.0	32.0	36.0
37	34.64192507989111	36.0	36.0	36.0	32.0	36.0
38	34.592806841046276	36.0	36.0	36.0	32.0	36.0
39	34.4566220854539	36.0	36.0	36.0	32.0	36.0
40	34.456133861995504	36.0	36.0	36.0	32.0	36.0
41	34.38300390578767	36.0	36.0	36.0	32.0	36.0
42	34.48800153864363	36.0	36.0	36.0	32.0	36.0
43	34.385089951473546	36.0	36.0	36.0	32.0	36.0
44	34.17697360634395	36.0	36.0	36.0	32.0	36.0
45	34.20151793111611	36.0	36.0	36.0	32.0	36.0
46	34.083693336489524	36.0	36.0	36.0	32.0	36.0
47	34.188025210084035	36.0	36.0	36.0	32.0	36.0
48	34.12288436501361	36.0	36.0	36.0	32.0	36.0
49	33.942478399810625	36.0	36.0	36.0	32.0	36.0
50	33.630681145697714	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	3.0
22	11.0
23	17.0
24	54.0
25	120.0
26	272.0
27	426.0
28	737.0
29	1072.0
30	1582.0
31	2240.0
32	3310.0
33	5159.0
34	10299.0
35	42288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92884837363483	21.1353479148785	12.444135318317697	24.49166839316897
2	16.211457655205066	21.59259040066284	36.428064153400015	25.76788779073208
3	17.18990413066635	27.691146881287725	27.30352704462067	27.815421943425257
4	12.535507160610724	15.86874186294236	34.52923422890283	37.066516747544085
5	16.398390342052313	34.69197538170198	30.69298141791928	18.21665285832643
6	32.184282163569655	35.93028760800095	16.41022606225589	15.475204166173512
7	27.879038939519468	31.60877026867085	21.45076340395313	19.06142738785655
8	25.825541484199316	33.73624097526334	19.67244644336608	20.765771097171264
9	26.241271156349868	13.196828026985441	20.478754882234583	40.08314593443011
10	17.913362528109836	25.486743993371995	27.344952065333178	29.254941413184994
11	35.69949106403125	24.68043555450349	19.974257308557224	19.645816072908037
12	23.838619955024264	24.933424073854894	27.586104864481005	23.641851106639837
13	28.232631080601255	23.624097526334477	23.726180613090307	24.41709077997396
14	22.622499704106993	17.47248195052669	25.652444076222036	34.252574269144276
15	27.534323588590365	24.80175168659013	21.40933838324062	26.254586341578882
16	27.08792590729535	27.413413028361767	20.773475758606917	24.72518530573597
17	22.453840691206057	26.95881169369156	26.12883181441591	24.458515800686474
18	22.814830157415077	28.026985442064152	24.701148064859744	24.457036335661027
19	25.202686708486212	22.694993490353887	26.688069594034797	25.414250207125104
20	27.668954905906023	22.77192567167712	23.593028760800095	25.966090661616757
21	28.012605228506754	24.248790519447855	23.78719060229912	23.951413649746268
22	25.94241922120961	24.581311397798554	25.9927210320748	23.483548348917033
23	23.820866374718904	26.282696177062377	25.797431648715826	24.0990057995029
24	24.387501479465026	22.144632500887678	26.804947331045092	26.6629186886022
25	26.12883181441591	23.4510001183572	23.33116345129601	27.08900461593088
26	26.262372209317807	24.85242118033466	23.84784956577059	25.03735704457694
27	23.176162506842626	25.689810773623705	27.946028317379536	23.187998402154133
28	23.73723923657346	26.039354934161857	25.68871134783252	24.534694481432165
29	23.973605953455344	23.399565030847302	26.40440295305588	26.222426062641475
30	25.11799257275592	22.996345559188626	25.26446611134948	26.621195756705973
31	25.211936500421654	25.331774940450654	23.739847021053098	25.716441538074598
32	22.856931498742416	25.03920698328155	27.063175025891407	25.040686492084628
33	24.027222961976623	25.4741825713863	26.239088622577306	24.259505844059774
34	23.45282655974908	22.732316432661154	27.605746327173737	26.209110680416032
35	26.493564136706617	23.37032105341027	24.561325639887556	25.57478916999556
36	27.501775358030535	24.674517694401704	22.650609539590484	25.173097407977274
37	23.102188161145715	25.28886981994644	26.641120859285998	24.967821159621842
38	22.918392709196354	25.179015268079063	26.948455438513435	24.95413658421115
39	23.92478288529538	21.930434525306623	27.453359175038095	26.6914234143599
40	26.46062345578553	23.8907546862748	24.509180216300987	25.139441641638683
41	27.14710538385288	25.26667751623737	23.26641120859286	24.319805891316893
42	23.403562762620584	25.995738888560098	26.29312895780316	24.307569391016155
43	23.189027638042255	27.20601290169853	25.112445996330713	24.492513463928507
44	24.087526445828587	22.76190617093992	26.21798760189966	26.93257978133183
45	25.732715894127917	22.748590788714473	25.393913390836058	26.12477992632155
46	27.97751146619322	25.531883414706318	22.06983281550525	24.420772303595207
47	23.168422298496864	25.392058231743402	25.929104035980586	25.510415433779144
48	22.886521674803966	26.816097055777483	24.80248557478917	25.49489569462938
49	24.01757708468959	23.23193466295792	26.27981298455347	26.470675267799017
50	25.54444312936442	24.704106994910642	24.40377559474494	25.347674280979998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	37.0
2	23.0
3	31.0
4	39.0
5	43.5
6	48.0
7	30.0
8	12.0
9	25.0
10	38.0
11	71.0
12	104.0
13	174.0
14	244.0
15	305.5
16	367.0
17	326.0
18	285.0
19	215.0
20	145.0
21	133.5
22	122.0
23	116.5
24	111.0
25	142.5
26	174.0
27	172.0
28	170.0
29	231.5
30	293.0
31	496.0
32	699.0
33	988.0
34	1277.0
35	1654.0
36	2031.0
37	2474.0
38	2917.0
39	3574.0
40	4231.0
41	4474.0
42	4717.0
43	4580.0
44	4443.0
45	4275.5
46	4108.0
47	4346.5
48	4585.0
49	4742.5
50	4900.0
51	4856.5
52	4813.0
53	4517.5
54	4222.0
55	3927.5
56	3633.0
57	3504.0
58	3375.0
59	3304.0
60	3233.0
61	3136.5
62	3040.0
63	2726.0
64	2412.0
65	2247.5
66	2083.0
67	1801.5
68	1520.0
69	1322.0
70	1124.0
71	865.0
72	606.0
73	634.0
74	662.0
75	513.5
76	365.0
77	267.0
78	169.0
79	121.5
80	74.0
81	52.5
82	31.0
83	51.0
84	71.0
85	43.0
86	15.0
87	9.0
88	3.0
89	1.5
90	0.0
91	1.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02663037045804237
2	0.005917860101787194
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0014794650254467984
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0014794650254467984
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0014794650254467984
27	0.0014794650254467984
28	0.002958930050893597
29	0.0014794650254467984
30	0.004438395076340395
31	0.0014794650254467984
32	0.002958930050893597
33	0.002958930050893597
34	0.0014794650254467984
35	0.002958930050893597
36	0.0
37	0.0014794650254467984
38	0.0
39	0.0014794650254467984
40	0.0014794650254467984
41	0.0014794650254467984
42	0.005917860101787194
43	0.005917860101787194
44	0.0014794650254467984
45	0.0014794650254467984
46	0.002958930050893597
47	0.0
48	0.002958930050893597
49	0.005917860101787194
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	67592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.019824831340987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.293184170321815	18.40010652148183
2	11.43856882361638	6.867676648124038
3	6.175151545019959	5.561309030654515
4	3.9919175989354883	4.793466682447627
5	2.9274062392193585	4.394011125576991
6	2.2128037060765857	3.985678778553675
7	1.6805480262185206	3.531483015741508
8	1.4242767729535262	3.420523138832998
9	1.1482923463604553	3.1024381583619363
>10	7.397368291360702	39.39519469759735
>50	0.2907693065891282	5.756598414013492
>100	0.019713173328076487	0.7915137886140372
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	210	0.3106876553438277	No Hit
CTAATAAACAGCATGATGAAGAAACGTTTCGCATCGAGAAAAATTGATCC	123	0.18197419812995622	No Hit
ATACTAGAGTCCGCAAAAAGGACCTAATAATCTCATTTGGTTCCCTCGTA	101	0.14942596757012663	No Hit
GTACTGGTCGTAGCGGTCGACGAACTGGTCCTCGAGCTCACCGATCTCGT	101	0.14942596757012663	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100	0.14794650254467984	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.14202864244289265	No Hit
ATGCTACCCTCACCTTTCGGGCTGGCTTGAATGATGCCCTTGAAGCTCTT	93	0.13759024736655226	No Hit
CTATAACCCTGCTGATACCCGCTACTTTGCCCGTACCCGGGAGCGTCGTT	86	0.12723399218842465	No Hit
ATCCTGGATGATGGCCTTGGAAGCCTCGTATCCGTCGTAGGCGTTGCGGG	84	0.12427506213753108	No Hit
GTCCTGGTCCTGACCAGAGAGGTCGTGGCCAGATGTTTGGTTGCGACGGC	84	0.12427506213753108	No Hit
GATCAAGGCAGTTCTGAGAGCGGGAGTGCTTGCCAGGCGGCATGTCTCAG	83	0.12279559711208428	No Hit
CCTCATAACCACCGCAAACCGCCAAAATGCTCGCCGCCCGCACCGCCACC	82	0.12131613208663747	No Hit
CACATACTCCATGCGCCAGTCGCGCGCGCCGACTGCCTCGCAGATCCAGA	81	0.11983666706119067	No Hit
GAATAAGCTAAGCCACCCTTCATTGATCAGGCTTTTCATTAATCTAGAAA	81	0.11983666706119067	No Hit
GTCTCATATCGCGCTTAAAATGGGTTGTCCTCTGGAGCAACCTCCTTGTT	80	0.11835720203574387	No Hit
ACCAAGCACTGTACGACTCTCTGCACGCCAAAGACCACACCTCATAACCA	79	0.11687773701029708	No Hit
GGGCTACAGCGGCGGACATGAGCAGCAGGTTTACGGAGCAGGAGGCTACC	76	0.11243934193395667	No Hit
CACATACACAGCAAAATACCTATCACACCGCAATCATGGACATGATCGGC	74	0.1094804118830631	No Hit
GTACATGGGGAGCTGTGAATGAGTTGGTTGTTCGAGAGGGAAGGCATTTT	73	0.1080009468576163	No Hit
GTGAATGAGTTGGTTGTTCGAGAGGGAAGGCATTTTCTTATAATGGCGTT	72	0.10652148183216947	No Hit
CTCTAAGACAAGAGAACCAACCCAATGCAAAACGCCATATGCTCGCAGAA	70	0.1035625517812759	No Hit
TTTTTATAGTGGGTGTTTCAGCTCCATCCGCGAGGGACCTCACCTACATA	69	0.1020830867558291	No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT	69	0.1020830867558291	No Hit
GGGTAGCATCGTGCACTGGACTTTGGAGTATGAAAAGTTGCACAACCAAA	69	0.1020830867558291	No Hit
CTCCCAGAGCGCCCATGCTCCCCAGTAAAACTCCGAAATGCTCCCTCTAA	68	0.1006036217303823	No Hit
ATCATGGACATGATCGGCAAGGTCGCTAAATCGCTGCTCGACAAGAACGG	68	0.1006036217303823	No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA	68	0.1006036217303823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0014794650254467984	0.0
6	0.0	0.0	0.0	0.0014794650254467984	0.0
7	0.0	0.0	0.0	0.0014794650254467984	0.0
8	0.0	0.0	0.0	0.0014794650254467984	0.0
9	0.0	0.0	0.0	0.0014794650254467984	0.0
10	0.0	0.0	0.0	0.0014794650254467984	0.0
11	0.0	0.0	0.0	0.0014794650254467984	0.0
12	0.0	0.0	0.0	0.0014794650254467984	0.0
13	0.0	0.0	0.0	0.0014794650254467984	0.0
14	0.0	0.0	0.0	0.0014794650254467984	0.0
15	0.0	0.0	0.0	0.0014794650254467984	0.0
16	0.0	0.0	0.0	0.0014794650254467984	0.0
17	0.0	0.0	0.0	0.0014794650254467984	0.0
18	0.0	0.0	0.0	0.0014794650254467984	0.0
19	0.0	0.0	0.0	0.0014794650254467984	0.0
20	0.0	0.0	0.0	0.0014794650254467984	0.0
21	0.0	0.0	0.0	0.0014794650254467984	0.0
22	0.0	0.0	0.0	0.0014794650254467984	0.0
23	0.0	0.0	0.0	0.0014794650254467984	0.0
24	0.0	0.0	0.0	0.0014794650254467984	0.0
25	0.0	0.0	0.0	0.0014794650254467984	0.0
26	0.0	0.0	0.0	0.0014794650254467984	0.0
27	0.0	0.0	0.0	0.0014794650254467984	0.0
28	0.0	0.0	0.0	0.002958930050893597	0.0
29	0.0	0.0	0.0	0.005917860101787194	0.0
30	0.0	0.0	0.0	0.010356255178127589	0.0
31	0.0	0.0	0.0	0.016274115279914785	0.0
32	0.0	0.0	0.0	0.03254823055982957	0.0
33	0.0	0.0	0.0	0.04290448573795715	0.0
34	0.0	0.0	0.0	0.060658066043318734	0.0
35	0.0	0.0	0.0	0.0858089714759143	0.0
36	0.0	0.0	0.0	0.11687773701029708	0.0
37	0.0	0.0	0.0	0.15978222274825424	0.0
38	0.0	0.0	0.0	0.22044028879157296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCTCG	20	7.803912E-4	44.0	35
CGGGAAA	20	7.803912E-4	44.0	40
ACCTCGG	20	7.803912E-4	44.0	36
GGGAAAA	20	7.803912E-4	44.0	41
AAGCATA	20	7.803912E-4	44.0	33
TCAAGGC	35	7.1889644E-6	37.714287	3
GCAGTTC	35	7.1889644E-6	37.714287	8
CAAGGCA	35	7.1889644E-6	37.714287	4
ATCAAGG	35	7.1889644E-6	37.714287	2
GTGCTTG	35	7.1889644E-6	37.714287	26
TGAGAGC	35	7.1889644E-6	37.714287	15
GCGGGAG	30	1.2881715E-4	36.666664	20
GGGAGTG	30	1.2881715E-4	36.666664	22
AGCGGGA	30	1.2881715E-4	36.666664	19
CGGGAGT	30	1.2881715E-4	36.666664	21
GAAAAGG	25	0.0023371524	35.2	43
AATGACA	25	0.0023371524	35.2	26
GTCTCAG	40	1.79489E-5	33.0	44
AAGGCAG	40	1.79489E-5	33.0	5
GCTTGCC	40	1.79489E-5	33.0	28
>>END_MODULE